A study on the interaction of nanoparticles with lipid membranes and their influence on membrane fluidity

In recent years, liposomes encapsulated with nanoparticles have found enormous scopes in various biomedical fields such as drug design, transport, imaging, targeted delivery and therapy. These applications require a clear understanding about the interaction of nanoparticles with cell membranes. The present work aims to investigate the effect of encapsulation of uncharged and positively charged nanoparticles in three different types of lipids such as1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine (SOPC),1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine and1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine(SOPC-POPS) mixture and archaeal lipids. Through the temperature dependent fluorescence anisotropy measurements, we have found that the entrapment of nanoparticles in the bilayer has decreased the lipid transition temperature and increased the membrane fluidity of all three types of lipid vesicles. The results were more predominant in SOPC-POPS mixture because of high density encapsulation of nanoparticles in the vesicles due to electrostatic interaction between negatively charged membrane and positively charged iron oxide nanoparticles.

[1]  Sukhen Das,et al.  A Study on the Modulation of the Phase Behavior of Lipid Aggregates—Effect of Some Metal Nanoparticles , 2011 .

[2]  M. Sentjurc,et al.  Stability of diether C(25,25) liposomes from the hyperthermophilic archaeon Aeropyrum pernix K1. , 2011, Chemistry and physics of lipids.

[3]  P. Raspor,et al.  Structural and physicochemical properties of polar lipids from thermophilic archaea , 2009, Applied Microbiology and Biotechnology.

[4]  S. Kim,et al.  N-hexanoyl chitosan stabilized magnetic nanoparticles: Implication for cellular labeling and magnetic resonance imaging , 2008, Journal of nanobiotechnology.

[5]  Kostas Kostarelos,et al.  Liposome-nanoparticle hybrids for multimodal diagnostic and therapeutic applications. , 2007, Nanomedicine.

[6]  Sung-Hee Park,et al.  Loading of gold nanoparticles inside the DPPC bilayers of liposome and their effects on membrane fluidities. , 2006, Colloids and surfaces. B, Biointerfaces.

[7]  Steve Granick,et al.  How to stabilize phospholipid liposomes (using nanoparticles). , 2006, Nano letters.

[8]  N. P. Ulrih,et al.  Optimization of growth for the hyperthermophilic archaeon Aeropyrum pernix on a small-batch scale. , 2005, Canadian journal of microbiology.

[9]  Lindsay E. Pell,et al.  Photoluminescence quenching of silicon nanoparticles in phospholipid vesicle bilayers , 2003 .

[10]  M. Mozetič,et al.  Electric-field controlled liposome formation with embedded superparamagnetic iron oxide nanoparticles. , 2012, Chemistry and physics of lipids.