Estrogen receptors are present in the epididymis of the rooster.

Our recent discovery that testicular germ cells and epididymal sperm contain active P450 aromatase suggests that the reproductive tract may be a target for estrogen. Therefore, the objective of this study was to determine if estrogen receptors (ER) are present in the avian epididymis using immunocytochemistry, northern blot analysis, and in situ hybridization. Immunoperoxidase staining for ER was found principally in nuclei of nonciliated epithelial cells of proximal and distal efferent ductules and the epididymis duct. The ciliated cells also appeared to be slightly positive in the efferent ductules. Week immunostaining was also observed in the connective tissue of the epididymis duct. Immunostaining was more intense in epithelial cells of the efferent ductules than in epithelial cells of the epididymal duct of connective tissue cells. Strong specific hybridization signals for ER mRNA corresponded to the same areas exhibiting immunocytochemical localization. The presence of ER mRNA in the epididymis was confirmed by northern blot analysis, which showed a single band corresponding to approximately 7.8 kb, similar to that found in chicken oviduct. Based on these data, we suggest that the efferent ducts of the rooster are a primary target for estrogen and that estrogen may have a role in the regulation of avian epididymal function.

[1]  J. Bahr,et al.  Seasonal changes in spermatogenesis and testicular steroidogenesis in the male black bear Ursus americanus. , 1997, Journal of reproduction and fertility.

[2]  J. Bahr,et al.  Mouse Epididymal Sperm Contain Active P 450 Aromatase Which Decreases as Sperm Traverse the Epididymis , 2004 .

[3]  J. Bahr,et al.  Rooster testicular germ cells and epididymal sperm contain P450 aromatase. , 1995, Biology of reproduction.

[4]  R. Hess,et al.  Structure and function of the ductuli efferentes: A review , 1994, Microscopy research and technique.

[5]  J. Kirby,et al.  Developmental expression of testis messenger ribonucleic acids in the rat following propylthiouracil-induced neonatal hypothyroidism. , 1994, Biology of reproduction.

[6]  S. Hayashi,et al.  Induction of estrogen receptor and cell division in genital tracts of male mice by neonatal exposure to diethylstilbestrol. , 1994, Reproductive toxicology.

[7]  K. Toda,et al.  Germ cells of the mouse testis express P450 aromatase. , 1993, Endocrinology.

[8]  R. Hess,et al.  Localization and activity of Na+, K+ ‐ATPase in the ductuli efferentes of the rat , 1992, The Anatomical record.

[9]  J. Gorski,et al.  Immunodetection of Estrogen Receptors in Fetal and Neonatal Female Mouse Reproductive Tracts , 1992 .

[10]  J. Garrett,et al.  Differential Patterns of Regulated Gene Expression in the Adult Rat Epididymis a , 1991, Annals of the New York Academy of Sciences.

[11]  G. Prins,et al.  Androgen receptor localization in different cell types of the adult rat prostate. , 1991, Endocrinology.

[12]  D. Veeramachaneni,et al.  Endocytosis of androgen-binding protein, clusterin, and transferrin in the efferent ducts and epididymis of the ram. , 1991, Journal of andrology.

[13]  P. Cooke,et al.  Estrogen receptor expression in developing epididymis, efferent ductules, and other male reproductive organs. , 1991, Endocrinology.

[14]  R. Brenner,et al.  Estrogen receptor in the ductuli efferentes, epididymis, and testis of rhesus and cynomolgus macaques. , 1990, Biology of reproduction.

[15]  R. Brenner,et al.  Estrogen and progestin receptors in the reproductive tract of male and female primates. , 1990, Biology of reproduction.

[16]  B. J. Danzo,et al.  Developmental changes in and hormonal regulation of estrogen and androgen receptors present in the rabbit epididymis. , 1988, Biology of reproduction.

[17]  L. Hermo,et al.  Role of apical tubules in endocytosis in nonciliated cells of the ductuli efferentes of the rat: a kinetic analysis. , 1988, The American journal of anatomy.

[18]  R. P. Amann,et al.  Regional and seasonal differences in concentrations of androgen and estrogen receptors in ram epididymal tissue. , 1988, Biology of reproduction.

[19]  J. Clulow,et al.  Studies of fluid and spermatozoal transport in the extratesticular genital ducts of the Japanese quail. , 1988, Journal of anatomy.

[20]  B. O’Malley,et al.  Structural organization and regulation of the chicken estrogen receptor. , 1987, Molecular endocrinology.

[21]  R. Jones,et al.  Structural differentiation and fluid reabsorption in the ductuli efferentes testis of the rat. , 1987, Australian journal of biological sciences.

[22]  J. Pasqualini,et al.  Ontogeny of steroid receptors in the reproductive system. , 1986, International review of cytology.

[23]  P. Cooke,et al.  Stromal-epithelial interactions in adult organs. , 1985, Cell differentiation.

[24]  R. Narbaitz,et al.  Estrogen receptors in the fetal mouse. , 1980, Journal of steroid biochemistry.

[25]  M. Younes,et al.  Steroid receptors in the canine epididymis. , 1979, Journal of reproduction and fertility.

[26]  D. Fawcett,et al.  Failure of exogenous androgen to prevent regression of the initial segments of the rat epididymis after efferent duct ligation or orchidectomy. , 1979, Biology of reproduction.

[27]  R. Jaffe,et al.  Collection of rete testis fluid from rats without previous efferent duct ligation. , 1979, Biology of reproduction.

[28]  J. McLachlan,et al.  Reproductive tract lesions in male mice exposed prenatally to diethylstilbestrol , 1975, Science.

[29]  M. D. Tingari.  The fine structure of the epithelial lining of the ex-current duct system of the testis of the domestic fowl (Gallus domesticus). , 1972, Quarterly Journal of Experimental Physiology and Cognate Medical Sciences.

[30]  M. D. Tingari.  On the structure of the epididymal region and ductus deferens of the domestic fowl (Gallus domesticus). , 1971, Journal of anatomy.