Effects of Portulacae Herba (PH) on Allergic Contact Dermatitis (ACD) Induced by DNCB in Mice

Objective : Portulacae Herba (PH) can clear away heat, detoxicate, cool down blood heat. The present study was designed to investigate the effects of PH on ACD induced by 2,4-dinitrochlorobenzene (DNCB) in mice. Method : In this experiment, the effects of PH on changes in body weights, thicknesses and weights of ear, thicknesses of dorsum skin, degree of symptoms on the dorsum skin, histopathological changes of ear and dorsum skin, spleen weights, TNF-α and IL-10 in serum were observed. And the effects on the proliferation rates of splenocytes were also investigated in vitro and in vivo. Results : 1. PH spread (PHS) group and PH spread plus administered (PHS+Adm) group didn't show any changes in weight and thickness of ear. 2. But in PHS and PHS+Adm group, thickness of dorsum skin decreased significantly. 3. And PHS and PHS+Adm group showed meaningful effectiveness on ACD symptoms like erythema, desquamation and keratinization. 4. In histopathological observation, hyperplasia and edema of spongy tissues were remarkably diminished in PHS and PHS+Adm group. 5. And PH reduced the proliferation rates of splenocytes in vivo and vitro study.

[1]  Young-Jong Lee,et al.  Anti-inflammatory Effect of Portulacae Herba Water Extract on Lipopolysaccharide-activated RAW 264.7 Macrophages , 2016 .

[2]  Bit-na-rae Kim,et al.  Effects of Cicadae Periostracum (CP) in Allergic Contact Dermatitis (ACD) Induced by DNCB in Mice , 2015 .

[3]  S. Ryu,et al.  Effects of Crataegus Pinnatifida (CP) on Allergic Contact Dermatitis (ACD) Induced by DNCB in Mice , 2014 .

[4]  Karimov Abdusamat Ismonovich,et al.  Effect of γ , 2013 .

[5]  Miyoung Kim,et al.  Effect of Lithospermi Radix on Contact Dermatitis Induced by Dinitrofluorobenzene in Mice , 2012, Journal of pharmacopuncture.

[6]  Hyeong Mi Kim,et al.  Anti-inflammatory and Anti-pruritic Effects of Portulaca oleracea L. Extract Using In Vitro and In Vivo Inflammation Model: LPS-treated Raw264.7 Cells, Keratinocytes, NC/Nga Mice and Hairless SKH-1 Mice , 2011 .

[7]  S. Lim,et al.  Effect of Scrophularia buergeriana Extract on the Degranulation of Mast Cells and Ear Swelling Induced by Dinitrofluorobenzene in Mice , 2011, Inflammation.

[8]  Soo-yeon Park,et al.  Effects of Scrophulariae Radix (SR) on Allergic Contact Dermatitis (ACD) induced by DNCB in mice , 2011 .

[9]  Xiaoying Yuan,et al.  Effects and mechanisms of aloperine on 2, 4-dinitrofluorobenzene-induced allergic contact dermatitis in BALB/c mice. , 2010, European journal of pharmacology.

[10]  Han-Sol Jeong,et al.  Anti-oxidative and Anti-inflammatory Effects of Protulaca Oleracea on the LPS-stimulated AGS Cells , 2009 .

[11]  Hee-taek Kim,et al.  The effects of Naetakchunkeum-san on the Allergic Contact Dermatitis induced by DNCB , 2009 .

[12]  Soo-yeon Park,et al.  Effects of Lonicerae Caulis (LC) on Gene Expression of Human melanoma cells , 2009 .

[13]  Chae-Hyun Kim,et al.  The Inhibitory Effects of Portulaca oleracea L. on HCl-ethanol Induced Gastritis in Rats , 2009 .

[14]  A. Morita,et al.  Experimental Extracorporeal Photopheresis Inhibits the Sensitization and Effector Phases of Contact Hypersensitivity via Two Mechanisms: Generation of IL-10 and Induction of Regulatory T Cells1 , 2008, The Journal of Immunology.

[15]  M. Shon,et al.  Effect of Eucommia ulmoides Extracts on Allergic Contact Dermatitis and Oxidative Damage Induced by Repeat Elicitation of DNCB , 2007 .

[16]  김종태,et al.  마치현 추출물이 3T3-L1 지방세포에서 지방 분해 및 Hormone Sensitive Lipase (HSL) 유전자 발현에 미치는 효과 , 2006 .

[17]  Y. Kwon,et al.  Effect of Lonicerae Flos-Skin on Contact Hypersensitivity induced by Repeat Elicitation of DNCB , 2006 .

[18]  이경우,et al.  Radiographic estimation of experimentally induced splenomegaly in dogs , 2006 .

[19]  Sung-Hwan Park,et al.  Selective Expansion of TCR $V{\beta}3$+CD4+T Cells in Collagen-induced Arthritis in DBA/1 Mice , 2005 .

[20]  Yoon-bum Kim,et al.  The Effects of Sanpunggosamhwan on the Allergic Contact Dermatitis , 2005 .

[21]  S. Yu,et al.  Herba Portulacae induced Apoptosis in Human CervicalCarcinoma HeLa Cells , 2005 .

[22]  Jin-Teak Kim,et al.  The Effects of Hangryunhaedocktang on Allergic Contact Dermatitis Based on the Morphological Changes in Epidemal Damage in Mice , 2004 .

[23]  K. B. Hicks,et al.  Phytosterols, phytostanols, and their conjugates in foods: structural diversity, quantitative analysis, and health-promoting uses. , 2002, Progress in lipid research.

[24]  Kim Dong-Eun,et al.  The effect of Rhemanniae radix extract on allergic contact dermatitis on Mice induced by DNCB , 2000 .

[25]  Ho,et al.  A Literature study on the external therapy of contact dermatitis. , 1998 .

[26]  G Frank Gerberick,et al.  Allergic Contact Dermatitis , 1998, Springer Berlin Heidelberg.

[27]  C. Fournier,et al.  Collagen-induced arthritis in DBA/1 mice: cytokine gene activation following immunization with type II collagen. , 1996, Cellular immunology.

[28]  K. Magnus,et al.  High Concentration of (−)-Noradrenaline in Portulaca oleracea L. , 1961, Nature.