Nocardia araoensis sp. nov. and Nocardia pneumoniae sp. nov., isolated from patients in Japan.

Two actinomycete strains isolated from two patients with lung nocardiosis between 1995 and 1997 in Japan were assigned to novel species of the genus Nocardia based on morphological and chemical criteria. Comparative 16S rRNA gene sequence analysis of the two strains revealed that they belong to the genus Nocardia and are most closely related to the species Nocardia beijingensis. Determination of DNA-DNA relatedness indicated that these strains could be assigned to two novel species. Based on their phenotypic and phylogenetic characters, two novel species of the genus Nocardia are proposed: Nocardia araoensis sp. nov. for IFM 0575(T) (=NBRC 100135(T)=JCM 12118(T)=DSM 44729(T)) and Nocardia pneumoniae sp. nov. for IFM 0784(T) (=NBRC 100136(T)=JCM 12119(T)=DSM 44730(T)).

[1]  K. Nishimura,et al.  First human case of nocardiosis caused by Nocardia pseudobrasiliensis in Japan , 2003, Mycopathologia.

[2]  D. Harmsen,et al.  Phylogeny of the Genus Nocardia Based on Reassessed 16S rRNA Gene Sequences Reveals Underspeciation and Division of Strains Classified as Nocardia asteroides into Three Established Species and Two Unnamed Taxons , 2003, Journal of Clinical Microbiology.

[3]  D. Kiska,et al.  Identification of Medically Relevant Nocardia Species with an Abbreviated Battery of Tests , 2002, Journal of Clinical Microbiology.

[4]  U. Steiner,et al.  Nocardia ignorata sp. nov. , 2001, International journal of systematic and evolutionary microbiology.

[5]  M. Goodfellow,et al.  Nocardia beijingensis sp. nov., a novel isolate from soil. , 2001, International journal of systematic and evolutionary microbiology.

[6]  Naoko Kinoshita,et al.  Nocardia vinacea sp. nov. , 2001 .

[7]  N. Nakanishi,et al.  Selective Isolation and Distribution of Actinomadura rugatobispora Strains in Soil , 2000 .

[8]  M. Goodfellow,et al.  The Nocardia salmonicida clade, including descriptions of Nocardia cummidelens sp. nov., Nocardia fluminea sp. nov. and Nocardia soli sp. nov. , 2000, Antonie van Leeuwenhoek.

[9]  J. Ishikawa,et al.  Colony PCR for Detection of Specific DNA Sequences in Actinomycetes , 2000 .

[10]  E. Stackebrandt,et al.  Proposal for a New Hierarchic Classification System, Actinobacteria classis nov. , 1997 .

[11]  Y. Tanaka,et al.  PathogenicNocardia isolated from clinical specimens including those of AIDS patients in Thailand , 1995, European Journal of Epidemiology.

[12]  J. Chun,et al.  A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. , 1995, International journal of systematic bacteriology.

[13]  J. Thompson,et al.  CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. , 1994, Nucleic acids research.

[14]  B. Dupont,et al.  Review of nocardial infections in France 1987 to 1990 , 1992, European Journal of Clinical Microbiology and Infectious Diseases.

[15]  Takayuki Ezaki,et al.  Fluorometric Deoxyribonucleic Acid-Deoxyribonucleic Acid Hybridization in Microdilution Wells as an Alternative to Membrane Filter Hybridization in which Radioisotopes Are Used To Determine Genetic Relatedness among Bacterial Strains , 1989 .

[16]  T. Kudo,et al.  Nocardia seriolae sp. nov. Causing Nocardiosis of Cultured Fish , 1988 .

[17]  Lawrence G. Wayne,et al.  International Committee on Systematic Bacteriology: Announcement of the Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics , 1988 .

[18]  N. Saitou,et al.  The neighbor-joining method: a new method for reconstructing phylogenetic trees. , 1987, Molecular biology and evolution.

[19]  K. Komagata,et al.  Determination of DNA base composition by reversed-phase high-performance liquid chromatography , 1984 .

[20]  L. Miller.  Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids , 1982, Journal of clinical microbiology.

[21]  M. Goodfellow,et al.  Numerical classification of sporoactinomycetes containing meso-diaminopimelic acid in the cell wall. , 1982, Journal of general microbiology.

[22]  D. Minnikin,et al.  Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria , 1980 .

[23]  J. Staneck,et al.  Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. , 1974, Applied microbiology.

[24]  Motoo Kimura,et al.  On the stochastic model for estimation of mutational distance between homologous proteins , 1972, Journal of Molecular Evolution.

[25]  Kubica Gp,et al.  The arylsulfatase activity of acid-fast bacilli. II. The differentiation of Mycobacterium avium from the unclassified group III nonphoto chromogenic mycobacteria. , 1961 .

[26]  Marguerita Sasser,et al.  Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids Technical Note # 101 , 2001 .

[27]  Max Sussman,et al.  Topley and Wilson's Microbiology and Microbial infections , 1998 .

[28]  Y. Mikami.  Susceptibility patterns of pathogenic Nocardia to some selected antimicrobial agents and their usefulness in the identification work in a clinical laboratory. , 1989 .

[29]  R. Gordon,et al.  Nocardia coeliaca, Nocardia autotrophica, and the Nocardin Strain , 1974 .

[30]  K. Miura,et al.  PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT. , 1963, Biochimica et biophysica acta.