Enzymatic Saccharification of Salix viminalis cv. Q683 Biomass for Bioethanol Production

The possibility of employing biomass of Salix viminalis cv. Q683 as a resource of bio-energy was evaluated. The chemical analysis of S. viminalis cv. Q683 leaf biomass showed components such as, extractives (2.57%), lignin (39.06%), hemicellulose (21.61%), and cellulose (37.83%), whereas, its stem was composed of extractives (1.67%), lignin (23.54%), hemicellulose (33.64%), and cellulose (42.03%). The biomass of S. viminalis cv. Q683 was saccharified using two enzymes celluclast and viscozyme. The saccharification of S. viminalis cv. Q683 biomass was influenced by enzymes and their strengths. The optimal enzyme combination was found to be celluclast (59 FPU/g substrate) and viscozyme (24 FBG/g substrate). On saccharification the glucose from leaf and stem biomass was 7.5g/L and 11.7g/L, respectively after 72 hr of enzyme treatment. The biomass and enzyme-treated biomass served as the feedstock for ethanol production by fermentation. The ethanol production from stem and leaf biomass was 5.8 g/L and 2.2 g/L respectively, while the fermentation of the enzymatic hydrolysates yielded 5 g/L to 8 g/L bioethanol in 72 hours.

[1]  A. Ragauskas,et al.  Comparative studies on hydrothermal pretreatment and enzymatic saccharification of leaves and internodes of alamo switchgrass. , 2011, Bioresource technology.

[2]  Ratna R. Sharma-Shivappa,et al.  Lime pretreatment of switchgrass at mild temperatures for ethanol production. , 2010, Bioresource technology.

[3]  Richard D. Taylor,et al.  2009 Outlook of the U.S. and World Sugar Markets, 2008-2018 , 2009 .

[4]  K. Heinsoo,et al.  Fine root biomass and production in a Salix viminalis and Salix dasyclados plantation. , 2009 .

[5]  Hongzhang Chen,et al.  Organosolv pretreatment by crude glycerol from oleochemicals industry for enzymatic hydrolysis of wheat straw. , 2008, Bioresource technology.

[6]  B. Ahring,et al.  Hydrolysis of Miscanthus for bioethanol production using dilute acid presoaking combined with wet explosion pre-treatment and enzymatic treatment. , 2008, Bioresource technology.

[7]  Ratna R. Sharma-Shivappa,et al.  A comparison of chemical pretreatment methods for improving saccharification of cotton stalks. , 2007, Bioresource technology.

[8]  P. Gray,et al.  Comparison of alkali and steam (acid) pretreatments of lignocellulosic materials to increase enzymic susceptibility: Evaluation under optimised pretreatment conditions , 2007 .

[9]  Anders Roos,et al.  Retreat from Salix - Swedish experience with energy crops in the 1990s , 2006 .

[10]  Michael E Himmel,et al.  The maize primary cell wall microfibril: a new model derived from direct visualization. , 2006, Journal of agricultural and food chemistry.

[11]  R. Bothast,et al.  Biotechnological processes for conversion of corn into ethanol , 2005, Applied Microbiology and Biotechnology.

[12]  A. Bjerre,et al.  Pretreatment of wheat straw using combined wet oxidation and alkaline hydrolysis resulting in convertible cellulose and hemicellulose , 2000, Biotechnology and bioengineering.

[13]  J. Saddler,et al.  Comparison of steam pretreatment of eucalyptus, aspen, and spruce wood chips and their enzymatic hydrolysis , 1992 .

[14]  J. Wright.  Ethanol from biomass by enzymatic hydrolysis , 1988 .

[15]  M. Moo-young,et al.  Alkali treatment of corn stover to improve sugar production by enzymatic hydrolysis , 1983, Biotechnology and bioengineering.

[16]  Choi InGyu,et al.  Study on Affecting Variables Appearing through Chemical Pretreatments of Poplar Wood (Populus euramericana) to Enzymatic Hydrolysis , 2009 .

[17]  Sandy Merino,et al.  Progress and challenges in enzyme development for biomass utilization. , 2007, Advances in biochemical engineering/biotechnology.

[18]  P. Savoie,et al.  Ethanol production from AFEX-treated forages and agricultural residues. , 1998, Applied Biochemistry and Biotechnology.

[19]  A. Wiselogel,et al.  Biomass feedstock resources and composition , 1997 .

[20]  W. D. Murray,et al.  Bioconversion of forest products industry waste cellulosics to fuel ethanol: a review. , 1996 .

[21]  B. E. Dale,et al.  Fermentation of lignocellulosic materials treated by ammonia freeze-explosion , 1984 .