Expression and release of interleukin-8 by human airway smooth muscle cells: inhibition by Th-2 cytokines and corticosteroids.

Interleukin (IL)-8 is a C-X-C chemokine that potently chemoattracts and activates neutrophils. We determined whether IL-8 could be produced by human airway smooth muscle cells in culture and examined its regulation. TNF-alpha stimulated IL-8 mRNA expression and protein release in a time- and dose-dependent manner, whereas IFN-gamma alone had no effect. Both cytokines together did not induce greater IL-8 release compared to TNF-alpha alone. IL-1beta was more potent in inducing IL-8 release and, together with TNF-alpha, there was a synergistic augmentation of IL-8 release. IL-8 release induced by TNF-alpha and IFN-gamma was partly inhibited by the Th-2-derived cytokines IL-4, IL-10, and IL-13, as well as by dexamethasone. In addition to its contractile responses, airway smooth muscle cells have synthetic and secretory potential with the release of IL-8 and subsequent recruitment and activation of neutrophils in the airways. Release of IL-8 can be modulated by Th-2-derived cytokines and corticosteroids.

[1]  K. Chung,et al.  Human airway smooth muscle cells express and release RANTES in response to T helper 1 cytokines: regulation by T helper 2 cytokines and corticosteroids. , 1997, Journal of immunology.

[2]  Hirst Sj.  Airway smooth muscle cell culture: application to studies of airway wall remodelling and phenotype plasticity in asthma. , 1996 .

[3]  K. Chung,et al.  Expression of RANTES in human airway epithelial cells: effect of corticosteroids and interleukin‐4, ‐10 and ‐13 , 1996, Immunology.

[4]  S. Hirst,et al.  Airway smooth muscle cell culture: application to studies of airway wall remodelling and phenotype plasticity in asthma. , 1996, The European respiratory journal.

[5]  K. Chung,et al.  Inhibition of macrophage inflammatory protein-1 alpha expression by IL-10. Differential sensitivities in human blood monocytes and alveolar macrophages. , 1995, Journal of immunology.

[6]  Konrad M. Hartung,et al.  IL-8 is expressed by human peripheral blood eosinophils. Evidence for increased secretion in asthma. , 1995, Journal of immunology.

[7]  E. Zelazny,et al.  IL-1 beta and IL-6 induce hyperplasia and hypertrophy of cultured guinea pig airway smooth muscle cells. , 1995, Journal of applied physiology.

[8]  H. Flad,et al.  IL-8 specifically binds to endothelial but not to smooth muscle cells. , 1995, Journal of immunology.

[9]  T. Schall,et al.  Regulation of the production of the RANTES chemokine by endothelial cells. Synergistic induction by IFN-gamma plus TNF-alpha and inhibition by IL-4 and IL-13. , 1995, Journal of immunology.

[10]  T. Harris,et al.  Tumor necrosis factor alpha modulates mitogenic responses of human cultured airway smooth muscle. , 1995, American journal of respiratory cell and molecular biology.

[11]  E. Puré,et al.  T lymphocytes adhere to airway smooth muscle cells via integrins and CD44 and induce smooth muscle cell DNA synthesis , 1994, The Journal of experimental medicine.

[12]  P. Collins,et al.  Zymosan-induced IL-8 release from human neutrophils involves activation via the CD11b/CD18 receptor and endogenous platelet-activating factor as an autocrine modulator. , 1994, Journal of immunology.

[13]  I. Adcock,et al.  Inhibition of interleukin-8 expression by dexamethasone in human cultured airway epithelial cells. , 1994, Immunology.

[14]  B. Dewald,et al.  Interleukin-8 and related chemotactic cytokines--CXC and CC chemokines. , 1994, Advances in immunology.

[15]  T. Standiford,et al.  Gene expression of macrophage inflammatory protein-1 alpha from human blood monocytes and alveolar macrophages is inhibited by interleukin-4. , 1993, American journal of respiratory cell and molecular biology.

[16]  S. McColl,et al.  Expression of the cytokine RANTES in human rheumatoid synovial fibroblasts. Differential regulation of RANTES and interleukin-8 genes by inflammatory cytokines. , 1993, The Journal of biological chemistry.

[17]  P. Barnes,et al.  Quantifying proliferation of cultured human and rabbit airway smooth muscle cells in response to serum and platelet-derived growth factor. , 1992, American journal of respiratory cell and molecular biology.

[18]  K. Matsushima,et al.  Molecular analysis of the inhibition of interleukin-8 production by dexamethasone in a human fibrosarcoma cell line. , 1992, Immunology.

[19]  M. Fey,et al.  Glucocorticoids downregulate gene expression of GM-CSF, NAP-1/IL-8, and IL-6, but not of M-CSF in human fibroblasts. , 1992, Blood.

[20]  R. Crystal,et al.  Interleukin-8 gene expression in human bronchial epithelial cells. , 1991, The Journal of biological chemistry.

[21]  T. Standiford,et al.  Interleukin-8 gene expression by a pulmonary epithelial cell line. A model for cytokine networks in the lung. , 1990, The Journal of clinical investigation.

[22]  K. Matsushima,et al.  Genomic structure of the human monocyte-derived neutrophil chemotactic factor IL-8. , 1989, Journal of immunology.

[23]  R. Strieter,et al.  Endothelial cell gene expression of a neutrophil chemotactic factor by TNF-alpha, LPS, and IL-1 beta. , 1989, Science.

[24]  P. Paré,et al.  The mechanics of airway narrowing in asthma. , 1989, The American review of respiratory disease.

[25]  C. van Breemen,et al.  Human airway smooth muscle in culture. , 1988, Tissue & cell.

[26]  J. Schröder,et al.  Purification and partial biochemical characterization of a human monocyte-derived, neutrophil-activating peptide that lacks interleukin 1 activity. , 1987, Journal of immunology.

[27]  K. Matsushima,et al.  Neutrophil chemotactic factor produced by lipopolysaccharide (LPS)-stimulated human blood mononuclear leukocytes: partial characterization and separation from interleukin 1 (IL 1). , 1987, Journal of immunology.

[28]  P. Chomczyński,et al.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. , 1987, Analytical biochemistry.