Drivers of biodiagnostic development

The promise of point-of-care medical diagnostics — tests that can be carried out at the site of patient care — is enormous, bringing the benefits of fast and reliable testing and allowing rapid decisions on the course of treatment to be made. To this end, much innovation is occurring in technologies for use in biodiagnostic tests. Assays based on nanomaterials, for example, are now beginning to make the transition from the laboratory to the clinic. But the potential for such assays to become part of routine medical testing depends on many scientific factors, including sensitivity, selectivity and versatility, as well as technological, financial and policy factors.

[1]  Ronald W. Davis,et al.  Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray , 1995, Science.

[2]  P. Alivisatos.  The use of nanocrystals in biological detection , 2004, Nature Biotechnology.

[3]  Joseph Wang,et al.  Ultrasensitive electrical biosensing of proteins and DNA: carbon-nanotube derived amplification of the recognition and transduction events. , 2004, Journal of the American Chemical Society.

[4]  K. Livak,et al.  Real time quantitative PCR. , 1996, Genome research.

[5]  E. Engvall,et al.  Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. , 1972, Journal of immunology.

[6]  Dr Ferdiye Taner,et al.  The enzyme-linked immunosorbent assay (ELISA). , 1976, Bulletin of the World Health Organization.

[7]  S. Jayasena.  Aptamers: an emerging class of molecules that rival antibodies in diagnostics. , 1999, Clinical chemistry.

[8]  Chad A. Mirkin,et al.  Nanostructures in Biodiagnostics , 2005 .

[9]  J. Szostak,et al.  In vitro selection of RNA molecules that bind specific ligands , 1990, Nature.

[10]  C. Lieber,et al.  Nanowire Nanosensors for Highly Sensitive and Selective Detection of Biological and Chemical Species , 2001, Science.

[11]  Chad A Mirkin,et al.  Colorimetric detection of mercuric ion (Hg2+) in aqueous media using DNA-functionalized gold nanoparticles. , 2007, Angewandte Chemie.

[12]  Gengfeng Zheng,et al.  Multiplexed electrical detection of cancer markers with nanowire sensor arrays , 2005, Nature Biotechnology.

[13]  T. Giorgio,et al.  Quantum dot self-assembly for protein detection with sub-picomolar sensitivity. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[14]  E. Engvall,et al.  Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. , 1971, Immunochemistry.

[15]  D. Henze,et al.  The role of amyloid-beta derived diffusible ligands (ADDLs) in Alzheimer's disease. , 2006, Current topics in medicinal chemistry.

[16]  Yi Lu,et al.  A colorimetric lead biosensor using DNAzyme-directed assembly of gold nanoparticles. , 2003, Journal of the American Chemical Society.

[17]  H. Klocker,et al.  Nanoparticle-based bio-barcode assay redefines “undetectable” PSA and biochemical recurrence after radical prostatectomy , 2009, Proceedings of the National Academy of Sciences.

[18]  J. Storhoff,et al.  Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles. , 1997, Science.

[19]  C. Mirkin,et al.  Nanoparticle-based detection in cerebral spinal fluid of a soluble pathogenic biomarker for Alzheimer's disease. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[20]  C. Mirkin,et al.  Nanoparticle-Based Bio-Bar Codes for the Ultrasensitive Detection of Proteins , 2003, Science.

[21]  Vincent M Rotello,et al.  Detection and identification of proteins using nanoparticle-fluorescent polymer 'chemical nose' sensors. , 2007, Nature nanotechnology.

[22]  A. Libchaber,et al.  Single-mismatch detection using gold-quenched fluorescent oligonucleotides , 2001, Nature Biotechnology.

[23]  Lin He,et al.  Colloidal Au-Enhanced Surface Plasmon Resonance for Ultrasensitive Detection of DNA Hybridization , 2000 .

[24]  D. Balding,et al.  HLA Sequence Polymorphism and the Origin of Humans , 2006 .

[25]  M. Shim,et al.  Noncovalent functionalization of carbon nanotubes for highly specific electronic biosensors , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[26]  S. Nie,et al.  Quantum dot bioconjugates for ultrasensitive nonisotopic detection. , 1998, Science.

[27]  C. Mirkin,et al.  Scanometric DNA array detection with nanoparticle probes. , 2000, Science.

[28]  A. Fagan,et al.  Multimodal techniques for diagnosis and prognosis of Alzheimer's disease , 2009, Nature.

[29]  J L West,et al.  A whole blood immunoassay using gold nanoshells. , 2003, Analytical chemistry.

[30]  Michael J Sailor,et al.  Biodegradable luminescent porous silicon nanoparticles for in vivo applications. , 2009, Nature materials.

[31]  C R Cantor,et al.  Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates. , 1992, Science.

[32]  Chad A Mirkin,et al.  Nano-flares: probes for transfection and mRNA detection in living cells. , 2007, Journal of the American Chemical Society.

[33]  Adam D. McFarland,et al.  Single Silver Nanoparticles as Real-Time Optical Sensors with Zeptomole Sensitivity , 2003 .

[34]  C. Mirkin,et al.  Nanoparticles with Raman spectroscopic fingerprints for DNA and RNA detection. , 2002, Science.

[35]  Jinghong Li,et al.  In situ amplified chemiluminescent detection of DNA and immunoassay of IgG using special-shaped gold nanoparticles as label. , 2006, Clinical chemistry.