Micro-machined piezoelectric membrane-based immunosensor array.

This paper reports a micro-machined piezoelectric membrane-based biosensor array for immunoassay. Goat immunoglobulin G (IgG) and HBsAg were immobilized as the probe molecules on the square piezoelectric membranes of the sensors that have dimensions of 3.5 microm x 500 microm x 500 microm. Due to the mass sensitive nature of these sensors, their resonant frequencies were depressed after the anti-goat IgG or anti-HBsAg was captured by the goat IgG or HBsAg. The resonant frequencies of the sensors were measured by an impedance analyzer. The experimental results demonstrate that the measured frequency change varies from 100 to 700 Hz, and the mass sensitivity of the device is estimated to be about 6.25 Hz/ng. A near linear relationship between the frequency change and the concentration of goat IgG was obtained, and the mass of the attached anti-goat IgG was calculated. The preliminary results discussed in this work indicate that the micro-machined piezoelectric membrane-based biosensor has a potential application as an immunosensor.

[1]  C. Steinem,et al.  Piezoelectric Mass-Sensing Devices as Biosensors-An Alternative to Optical Biosensors? , 2000, Angewandte Chemie.

[2]  E. A. Wachter,et al.  Detection of mercury vapor using resonating microcantilevers , 1995 .

[3]  Il-Hoon Cho,et al.  Label-free protein assay with site-directly immobilized antibody using self-actuating PZT cantilever , 2005, The 13th International Conference on Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05..

[4]  Le Deng,et al.  Microbalance-DNA probe method for the detection of specific bacteria in water , 2002 .

[5]  Jung Ho Park,et al.  In-situ quantitative analysis of a prostate-specific antigen (PSA) using a nanomechanical PZT cantilever. , 2004, Lab on a chip.

[6]  Liviu Nicu,et al.  Resonating piezoelectric membranes for microelectromechanically based bioassay: detection of streptavidin–gold nanoparticles interaction with biotinylated DNA , 2005 .

[7]  Zhihong Wang,et al.  Exact solution of multilayered piezoelectric diaphragms. , 2003, IEEE transactions on ultrasonics, ferroelectrics, and frequency control.

[8]  Zhimin Li,et al.  Development of biosensor based on microdiaphragm , 2004, SPIE Smart Structures and Materials + Nondestructive Evaluation and Health Monitoring.

[9]  K. Hashimoto,et al.  Quantitative analysis for solid-phase hybridization reaction and binding reaction of DNA binder to hybrids using a quartz crystal microbalance , 1996 .

[10]  M. Grattarola,et al.  Micromechanical cantilever-based biosensors , 2001 .

[11]  O. Wolfbeis,et al.  Capacitive monitoring of protein immobilization and antigen-antibody reactions on monomolecular alkylthiol films on gold electrodes. , 1997, Biosensors & bioelectronics.

[12]  H. Craighead,et al.  Mechanical resonant immunospecific biological detector , 2000 .

[13]  Olivier Lazcka,et al.  Pathogen detection: a perspective of traditional methods and biosensors. , 2007, Biosensors & bioelectronics.

[14]  Geunbae Lim,et al.  A self-excited micro cantilever biosensor actuated by PZT using the mass micro balancing technique , 2006 .

[15]  Kui Yao,et al.  Measurement of longitudinal piezoelectric coefficient of thin films by a laser-scanning vibrometer , 2003, IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control.

[16]  H. Hng,et al.  Low Temperature Processing of Nanocrystalline Lead Zirconate Titanate (PZT) Thick Films and Ceramics by a Modified Sol-Gel Route , 2002 .

[17]  A. Karyakin,et al.  Oriented immobilization of antibodies onto the gold surfaces via their native thiol groups. , 2000, Analytical chemistry.

[18]  Sara Tombelli,et al.  Quartz crystal microbalance (QCM) affinity biosensor for genetically modified organisms (GMOs) detection. , 2003, Biosensors & bioelectronics.

[19]  Zhihong Wang,et al.  Dense PZT thick films derived from sol-gel based nanocomposite process , 2003 .

[20]  Richard M. White,et al.  Analytic comparison of the sensitivities of bulk‐wave, surface‐wave, and flexural plate‐wave ultrasonic gravimetric sensors , 1989 .

[21]  Gwo-Bin Lee,et al.  Microfluidic systems integrated with two-dimensional surface plasmon resonance phase imaging systems for microarray immunoassay. , 2007, Biosensors & bioelectronics.

[22]  Laura M. Lechuga,et al.  Nanomechanical biosensors: a new sensing tool , 2006 .

[23]  W. Göpel,et al.  Bioelectronic noses: a status report. Part II1Co-ordinating authors: Ch. Ziegler and W. Göpel. All other authors are contributing authors.1 , 1998 .