β-Adrenoceptor subtypes in sections of rat and guinea-pig kidney

SummaryThe present autoradiographical study examines the distribution of the two β-adrenoceptor subtypes in sections of rat and guinea-pig kidney. The radioligand [125Iodo]-(-)-cyanopindolol was used for the labelling of β-adrenoceptors and the selective β-adrenoceptor blocking agents ICI 89-406 (β1-antagonist) and ICI 118-551 (β2-antagonist) were utilized to differentiate both subclasses unequivocally. β-Adrenoceptors in rat kidney were found to be almost exclusively β1. They were located mainly on glomeruli and to a lesser extent on the straight part of the distal tubules and on the cortical portion of the collecting ducts. Some β2-adrenoceptors were localized around the corticomedullary junction. Grain localization in the autoradiograms was absent in the inner medulla and papilla. Glomeruli and distal tubules of the guinea-pig kidney also possess only β1-adrenoceptors, but, in contrast to the rat, extremely high concentrations of β2-adrenoceptors were associated with the straight part of the proximal tubules in the cortex and possibly with the cortical portion of the collecting duct. Labelling was not detected on the proximal convoluted tubule in either species.

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