Single-Bead Fluorescence Microspectroscopy: Detection of Self-Quenching in Fluorescence-Labeled Resin Beads

We have characterized two sets of fluorescence-labeled beads with single-bead fluorescence microspectroscopy. Our results demonstrate the possible self-quenching effect on the fluorescence in the resin bead and, therefore, call for a careful selection of fluorophores and a rigorous control of the labeling reaction yield in order to generate labeled beads for combinatorial chemistry and other disciplines.