Contrast-based sensorless adaptive optics for retinal imaging.

Conventional adaptive optics ophthalmoscopes use wavefront sensing methods to characterize ocular aberrations for real-time correction. However, there are important situations in which the wavefront sensing step is susceptible to difficulties that affect the accuracy of the correction. To circumvent these, wavefront sensorless adaptive optics (or non-wavefront sensing AO; NS-AO) imaging has recently been developed and has been applied to point-scanning based retinal imaging modalities. In this study we show, for the first time, contrast-based NS-AO ophthalmoscopy for full-frame in vivo imaging of human and animal eyes. We suggest a robust image quality metric that could be used for any imaging modality, and test its performance against other metrics using (physical) model eyes.

[1]  P Artal,et al.  Dynamics of the eye's wave aberration. , 2001, Journal of the Optical Society of America. A, Optics, image science, and vision.

[2]  David Williams,et al.  Adaptive optics retinal imaging in the living mouse eye , 2012, Biomedical optics express.

[3]  Emmanuel Beaurepaire,et al.  Accuracy of correction in modal sensorless adaptive optics. , 2012, Optics express.

[4]  David Williams,et al.  Morphology and topography of retinal pericytes in the living mouse retina using in vivo adaptive optics imaging and ex vivo characterization. , 2013, Investigative ophthalmology & visual science.

[5]  Jerome Mertz,et al.  Optically sectioned in vivo imaging with speckle illumination HiLo microscopy. , 2011, Journal of biomedical optics.

[6]  Austin Roorda,et al.  Noninvasive visualization and analysis of parafoveal capillaries in humans. , 2010, Investigative ophthalmology & visual science.

[7]  Jerome Mertz,et al.  Wide-field fluorescence sectioning with hybrid speckle and uniform-illumination microscopy. , 2008, Optics letters.

[8]  R. Zawadzki,et al.  Wavefront sensorless modal deformable mirror correction in adaptive optics: optical coherence tomography. , 2013, Optics letters.

[9]  R. Muller,et al.  Real-time correction of atmospherically degraded telescope images through image sharpening , 1974 .

[10]  Phillip Bedggood,et al.  Limitations to adaptive optics image quality in rodent eyes , 2012, Biomedical optics express.

[11]  T. Hebert,et al.  Adaptive optics scanning laser ophthalmoscopy. , 2002, Optics express.

[12]  M. Booth.  Wavefront sensorless adaptive optics for large aberrations. , 2007, Optics letters.

[13]  W. O. Saxton,et al.  Measurement of the Transverse Electric Field Profile of Light by a Self-referencing Method with Direct Phase Determination References and Links " Measurement of Atmospheric Wavefront Distortion Using Scattered Light from a Laser Guide-star, " , 2022 .

[14]  H. Grady Rylander,et al.  Maintaining the cornea and the general physiological environment in visual neurophysiology experiments , 2001, Journal of Neuroscience Methods.

[15]  J. Porter,et al.  Wavefront sensorless adaptive optics ophthalmoscopy in the human eye , 2011, Optics express.

[16]  Michelle Cua,et al.  In vivo imaging of human photoreceptor mosaic with wavefront sensorless adaptive optics optical coherence tomography. , 2015, Biomedical optics express.

[17]  D R Williams,et al.  Supernormal vision and high-resolution retinal imaging through adaptive optics. , 1997, Journal of the Optical Society of America. A, Optics, image science, and vision.

[18]  Martin J Booth,et al.  Image based adaptive optics through optimisation of low spatial frequencies. , 2007, Optics express.

[19]  Yifan Jian,et al.  Adaptive optics optical coherence tomography for in vivo mouse retinal imaging , 2013, Journal of biomedical optics.

[20]  J J Miller,et al.  Aberration correction by maximizing generalized sharpness metrics. , 2003, Journal of the Optical Society of America. A, Optics, image science, and vision.

[21]  Phillip Bedggood,et al.  System design considerations to improve isoplanatism for adaptive optics retinal imaging. , 2010, Journal of the Optical Society of America. A, Optics, image science, and vision.

[22]  Yifan Jian,et al.  Wavefront sensorless adaptive optics optical coherence tomography for in vivo retinal imaging in mice. , 2014, Biomedical optics express.

[23]  Norberto López-Gil,et al.  Ocular wave-front aberration statistics in a normal young population , 2002, Vision Research.

[24]  Phillip Bedggood,et al.  Optical Imaging of Human Cone Photoreceptors Directly Following the Capture of Light , 2013, PloS one.

[25]  M M La Vail,et al.  Survival of some photoreceptor cells in albino rats following long-term exposure to continuous light. , 1976, Investigative ophthalmology.

[26]  Phillip Bedggood,et al.  Improving high resolution retinal image quality using speckle illumination HiLo imaging , 2014, Biomedical optics express.

[27]  J. Hardy,et al.  Active optics: A new technology for the control of light , 1978, Proceedings of the IEEE.

[28]  M. Lavail,et al.  Rods and cones in the mouse retina. I. Structural analysis using light and electron microscopy , 1979, The Journal of comparative neurology.

[29]  Jerome Mertz,et al.  Optically sectioned fluorescence endomicroscopy with hybrid-illumination imaging through a flexible fiber bundle. , 2009, Journal of biomedical optics.

[30]  Phillip Bedggood,et al.  Direct visualization and characterization of erythrocyte flow in human retinal capillaries , 2012, Biomedical optics express.

[31]  Jerome Mertz,et al.  Fast optically sectioned fluorescence HiLo endomicroscopy. , 2012, Journal of biomedical optics.

[32]  Phillip Bedggood,et al.  Variability in bleach kinetics and amount of photopigment between individual foveal cones. , 2012, Investigative ophthalmology & visual science.

[33]  Alfredo Dubra,et al.  Non-common path aberration correction in an adaptive optics scanning ophthalmoscope. , 2014, Biomedical optics express.

[34]  David R Williams,et al.  In vivo imaging of microscopic structures in the rat retina. , 2009, Investigative ophthalmology & visual science.

[35]  Tony Wilson,et al.  Image-based adaptive optics for two-photon microscopy. , 2009, Optics letters.

[36]  J. W. Hardy,et al.  Active optics: A new technology for the control of light , 1978, Proceedings of the IEEE.

[37]  David H Sliney,et al.  Maximum permissible exposures for ocular safety (ANSI 2000), with emphasis on ophthalmic devices. , 2007, Journal of the Optical Society of America. A, Optics, image science, and vision.

[38]  Phillip Bedggood,et al.  Characteristics of the human isoplanatic patch and implications for adaptive optics retinal imaging. , 2008, Journal of biomedical optics.

[39]  Austin Roorda,et al.  Applications of Adaptive Optics Scanning Laser Ophthalmoscopy , 2010, Optometry and vision science : official publication of the American Academy of Optometry.

[40]  David R. Williams,et al.  Optical properties of the mouse eye , 2011, Biomedical optics express.