Control of the in vivo biodistribution of hybrid nanoparticles with different poly(ethylene glycol) coatings.

Fluorescent nanoparticles containing a gadolinium oxide core are very attractive because they are able to combine both imaging (fluorescence imaging, magnetic resonance imaging) and therapy (X-ray therapy and neutron-capture therapy) techniques. The exploitation of these multifunctional particles for in vivo applications requires accurate control of their biodistribution. The postfunctionalization of these particles by four different poly(ethylene glycol) derivatives, which differ by chain length and end group, exerts a great influence on the zeta potential of the nanoparticles and on their biodistribution after intravenous injection to HEK-beta3-tumor-bearing mice. This study reveals that the behavior of PEGylated nanoparticles, which was monitored by in vivo fluorescence imaging, depends on both the chain length and the end group of the PEG chain.

[1]  Klaas Nicolay,et al.  Quantum dots with a paramagnetic coating as a bimodal molecular imaging probe. , 2006, Nano letters.

[2]  Hooisweng Ow,et al.  Bright and stable core-shell fluorescent silica nanoparticles. , 2005, Nano letters.

[3]  P. Perriat,et al.  Functionalization of luminescent aminated particles for facile bioconjugation. , 2008, ACS nano.

[4]  M. Bawendi,et al.  Renal clearance of quantum dots , 2007, Nature Biotechnology.

[5]  R. Weissleder.  A clearer vision for in vivo imaging , 2001, Nature Biotechnology.

[6]  Cheuk Y. Tang,et al.  Improved biocompatibility and pharmacokinetics of silica nanoparticles by means of a lipid coating: a multimodality investigation. , 2008, Nano letters.

[7]  A. Grichine,et al.  In Vivo Noninvasive Optical Imaging of Receptor-Mediated RGD Internalization Using Self-Quenched Cy5-Labeled RAFT-c(-RGDfK-)4 , 2007, Molecular imaging.

[8]  Byron Ballou,et al.  Noninvasive imaging of quantum dots in mice. , 2004, Bioconjugate chemistry.

[9]  Chenjie Xu,et al.  Controlled PEGylation of Monodisperse Fe3O4 Nanoparticles for Reduced Non‐Specific Uptake by Macrophage Cells , 2007 .

[10]  D. Boturyn,et al.  Noninvasive Optical Imaging of Ovarian Metastases Using Cy5-labeled RAFT-c(-RGDfK-)4 , 2006, Molecular Imaging.

[11]  Itamar Willner,et al.  Integrated nanoparticle-biomolecule hybrid systems: synthesis, properties, and applications. , 2004, Angewandte Chemie.

[12]  Francis Vocanson,et al.  Gadolinium chelate coated gold nanoparticles as contrast agents for both X-ray computed tomography and magnetic resonance imaging. , 2008, Journal of the American Chemical Society.

[13]  Leaf Huang,et al.  Pharmacokinetics and biodistribution of nanoparticles. , 2008, Molecular pharmaceutics.

[14]  Ralph Weissleder,et al.  Nanoparticle PET-CT Imaging of Macrophages in Inflammatory Atherosclerosis , 2008, Circulation.

[15]  O. Tillement,et al.  Nanosized hybrid particles with double luminescence for biological labeling , 2005 .

[16]  A. Wear.  CIRCULATION , 1964, The Lancet.

[17]  Eric Pridgen,et al.  Factors Affecting the Clearance and Biodistribution of Polymeric Nanoparticles , 2008, Molecular pharmaceutics.

[18]  Michelle Bradbury,et al.  Fluorescent silica nanoparticles with efficient urinary excretion for nanomedicine. , 2009, Nano letters.

[19]  Alain Brenier,et al.  Synthesis and luminescent properties of sub-5-nm lanthanide oxides nanoparticles , 2003 .

[20]  Vladimir P Torchilin,et al.  Liposome clearance in mice: the effect of a separate and combined presence of surface charge and polymer coating. , 2002, International journal of pharmaceutics.

[21]  Parag Aggarwal,et al.  Preclinical studies to understand nanoparticle interaction with the immune system and its potential effects on nanoparticle biodistribution. , 2008, Molecular pharmaceutics.

[22]  P. Perriat,et al.  Hybrid gadolinium oxide nanoparticles: multimodal contrast agents for in vivo imaging. , 2007, Journal of the American Chemical Society.

[23]  Claudia Calcagno,et al.  Nanocrystal core high-density lipoproteins: a multimodality contrast agent platform. , 2008, Nano letters.

[24]  Chin-Tu Chen,et al.  Near‐Infrared Mesoporous Silica Nanoparticles for Optical Imaging: Characterization and In Vivo Biodistribution , 2009 .

[25]  Sangjin Park,et al.  Antibiofouling polymer-coated gold nanoparticles as a contrast agent for in vivo X-ray computed tomography imaging. , 2007 .

[26]  J. M. Harris,et al.  Effect of pegylation on pharmaceuticals , 2003, Nature Reviews Drug Discovery.

[27]  O Tillement,et al.  Synthesis and properties of europium-based phosphors on the nanometer scale: Eu2O3, Gd2O3:Eu, and Y2O3:Eu. , 2004, Journal of colloid and interface science.

[28]  Olivier Tillement,et al.  Hybrid gadolinium oxide nanoparticles combining imaging and therapy , 2009 .

[29]  Ralph Weissleder,et al.  Tat peptide-derivatized magnetic nanoparticles allow in vivo tracking and recovery of progenitor cells , 2000, Nature Biotechnology.

[30]  Weibo Cai,et al.  Nanoplatforms for targeted molecular imaging in living subjects. , 2007, Small.