Gga-miR-205a Affecting Myoblast Proliferation and Differentiation by Targeting CDH11

Non-coding RNAs especially miRNAs have been found to play important roles during skeletal muscle development. Our previous RNA-Seq performed on breast muscle tissue from 7 weeks old Recessive White Rock and Xinhua Chicken and leg muscle tissue from female Xinghua Chicken at three development time points (11 embryo age, 16 embryo age, and 1 day post hatch) (accession number GSE62971 and GSE89355, respectively) showed that miR-205a and CDH11 were differentially expressed genes. In this study, we found that overexpression of CDH11 significantly facilitated Quail muscle clone (QM7) and chicken primary myoblast (CPM) proliferation and hampered CPM differentiation. MiR-205a can directly binding to the 3′UTR of CDH11 and the overexpression of miR-205a could inhibit both cell lines (QM7) and CPM proliferation, at the meantime promote the differentiation of myoblasts. The Dual-Luciferase Reporter Assay results and qRT-PCR results showed that myogenin (MyoG) could regulate the expression of miR-205a by binding to the active region of miR-205a. Altogether our data suggest that MyoG could stimulate miR-205a expression to suppress CDH11, which promotes myoblasts proliferation while represses the differentiation.

[1]  Q. Nie,et al.  Characterization of miRNA and their target gene during chicken embryo skeletal muscle development , 2017, Oncotarget.

[2]  N. Szewczyk,et al.  An overview of technical considerations for Western blotting applications to physiological research , 2017, Scandinavian journal of medicine & science in sports.

[3]  M. Gautel,et al.  Transcriptional mechanisms regulating skeletal muscle differentiation, growth and homeostasis , 2011, Nature Reviews Molecular Cell Biology.

[4]  F. Logerfo,et al.  A novel function for cadherin 11/osteoblast-cadherin in vascular smooth muscle cells: modulation of cell migration and proliferation. , 2007, Journal of vascular surgery.

[5]  E. Olson,et al.  A gene with homology to the myc similarity region of MyoD1 is expressed during myogenesis and is sufficient to activate the muscle differentiation program. , 1990, Genes & development.

[6]  R. Sandaltzopoulos,et al.  Roles of NF-κB Signaling in the Regulation of miRNAs Impacting on Inflammation in Cancer , 2018, Biomedicines.

[7]  J. Montero,et al.  Decorin gene expression in the differentiation of the skeletal connective tissues of the developing limb. , 2014, Gene expression patterns : GEP.

[8]  S. Andreadis,et al.  Cadherin-11 regulates both mesenchymal stem cell differentiation into smooth muscle cells and the development of contractile function in vivo , 2014, Journal of Cell Science.

[9]  Charles Kooperberg,et al.  Global and gene‐specific analyses show distinct roles for Myod and Myog at a common set of promoters , 2006, The EMBO journal.

[10]  Q. Nie,et al.  E2F1-miR-20a-5p/20b-5p auto-regulatory feedback loop involved in myoblast proliferation and differentiation , 2016, Scientific Reports.

[11]  Q. Nie,et al.  miRNA-223 upregulated by MYOD inhibits myoblast proliferation by repressing IGF2 and facilitates myoblast differentiation by inhibiting ZEB1 , 2017, Cell Death and Disease.

[12]  G. Goodall,et al.  MiRNA-205 modulates cellular invasion and migration via regulating zinc finger E-box binding homeobox 2 expression in esophageal squamous cell carcinoma cells , 2011, Journal of Translational Medicine.

[13]  Li Kang,et al.  Identification of miRNAs associated with sexual maturity in chicken ovary by Illumina small RNA deep sequencing , 2013, BMC Genomics.

[14]  Giulio Gabbiani,et al.  Mechanisms of force generation and transmission by myofibroblasts. , 2003, Current opinion in biotechnology.

[15]  Rajvir Dahiya,et al.  MicroRNA‐205–directed transcriptional activation of tumor suppressor genes in prostate cancer , 2010, Cancer.

[16]  C. Redies,et al.  Restricted expression of classic cadherins in the spinal cord of the chicken embryo , 2014, Front. Neuroanat..

[17]  G. Goodall,et al.  The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1 , 2008, Nature Cell Biology.

[18]  Y. Azuma,et al.  Targeted Disruption of Cadherin‐11 Leads to a Reduction in Bone Density in Calvaria and Long Bone Metaphyses , 2001, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[19]  Hideki Makishima,et al.  [Deep sequencing]. , 2013, [Rinsho ketsueki] The Japanese journal of clinical hematology.

[20]  G. Crabtree,et al.  Interaction of a liver-specific nuclear factor with the fibrinogen and alpha 1-antitrypsin promoters. , 1987, Science.

[21]  Yang Luo,et al.  N‐cadherin is not essential for limb mesenchymal chondrogenesis , 2005, Developmental dynamics : an official publication of the American Association of Anatomists.

[22]  B. Hinz,et al.  Myofibroblast development is characterized by specific cell-cell adherens junctions. , 2004, Molecular biology of the cell.

[23]  T. Sakamaki,et al.  ErbB2 down-regulates microRNA-205 in breast cancer. , 2011, Biochemical and biophysical research communications.

[24]  M. Daidone,et al.  miR-205 Exerts tumor-suppressive functions in human prostate through down-regulation of protein kinase Cepsilon. , 2009, Cancer research.

[25]  Hailong Wu,et al.  Suppression of cell growth and invasion by miR-205 in breast cancer , 2008, Cell Research.

[26]  S. Majid,et al.  miRNA-205 Suppresses Melanoma Cell Proliferation and Induces Senescence via Regulation of E2F1 Protein* , 2011, The Journal of Biological Chemistry.

[27]  C. Burge,et al.  Conserved Seed Pairing, Often Flanked by Adenosines, Indicates that Thousands of Human Genes are MicroRNA Targets , 2005, Cell.

[28]  Thomas D. Schmittgen,et al.  Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. , 2001, Methods.

[29]  E. Olson,et al.  MicroRNAs flex their muscles. , 2008, Trends in genetics : TIG.

[30]  Jason I. Herschkowitz,et al.  The ups and downs of miR-205: Identifying the roles of miR-205 in mammary gland development and breast cancer , 2010, RNA biology.

[31]  F. Lecanda,et al.  Relative abundance of different cadherins defines differentiation of mesenchymal precursors into osteogenic, myogenic, or adipogenic pathways , 2000, Journal of cellular biochemistry.

[32]  Q. Nie,et al.  Myomaker, Regulated by MYOD, MYOG and miR-140-3p, Promotes Chicken Myoblast Fusion , 2015, International journal of molecular sciences.

[33]  R. Civitelli,et al.  N-cadherin and cadherin 11 modulate postnatal bone growth and osteoblast differentiation by distinct mechanisms , 2010, Journal of Cell Science.

[34]  T. Yatskievych,et al.  MicroRNA expression during chick embryo development , 2006, Developmental dynamics : an official publication of the American Association of Anatomists.

[35]  R. Dahiya,et al.  MicroRNA-205 inhibits Src-mediated oncogenic pathways in renal cancer. , 2011, Cancer research.

[36]  Xiaomei He,et al.  Deep Sequencing Analysis of miRNA Expression in Breast Muscle of Fast-Growing and Slow-Growing Broilers , 2015, International journal of molecular sciences.