The molecular toolbox for chromosomal heterologous multiprotein expression in Escherichia coli.

Heterologous multiprotein expression is the tool to answer a number of questions in basic science as well as to convert strains into producers and/or consumers of certain compounds in applied sciences. Multiprotein expression can be driven by plasmids with the disadvantages that the gene dosage might, in some cases, lead to toxic effects and that the continuous addition of antibiotics is undesirable. Stable genomic expression of proteins can forgo these problems and is a helpful and promising tool in synthetic biology. In the present paper, we provide an extract of methods from the toolbox for chromosome-based heterologous expression in Escherichia coli.

[1]  K. Terpe.  Overview of bacterial expression systems for heterologous protein production: from molecular and biochemical fundamentals to commercial systems , 2006, Applied Microbiology and Biotechnology.

[2]  J. Glasner,et al.  Gene replacement without selection: regulated suppression of amber mutations in Escherichia coli. , 2003, Gene.

[3]  Giuseppe Testa,et al.  DNA cloning by homologous recombination in Escherichia coli , 2000, Nature Biotechnology.

[4]  B. Ames,et al.  Positive selection of mutants with deletions of the gal-chl region of the Salmonella chromosome as a screening procedure for mutagens that cause deletions , 1975, Journal of bacteriology.

[5]  M. Wubbolts,et al.  An Alkane-Responsive Expression System for the Production of Fine Chemicals , 1999, Applied and Environmental Microbiology.

[6]  B. Wanner,et al.  Conditional-Replication, Integration, Excision, and Retrieval Plasmid-Host Systems for Gene Structure-Function Studies of Bacteria , 2001, Journal of bacteriology.

[7]  R. Schleif,et al.  DNA-dependent renaturation of an insoluble DNA binding protein. Identification of the RhaS binding site at rhaBAD. , 1994, Journal of molecular biology.

[8]  G. Phillips,et al.  Genetic system for reversible integration of DNA constructs and lacZ gene fusions into the Escherichia coli chromosome. , 2000, Plasmid.

[9]  M. Leiby,et al.  A New Positive/Negative Selection Scheme for Precise BAC Recombineering , 2009, Molecular biotechnology.

[10]  R. Hoess,et al.  Bacteriophage P1 site-specific recombination. II. Recombination between loxP and the bacterial chromosome. , 1981, Journal of molecular biology.

[11]  I. Blomfield,et al.  Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature‐sensitive pSC101 replicon , 1991, Molecular microbiology.

[12]  Rino Rappuoli,et al.  Counterselectable Markers: Untapped Tools for Bacterial Genetics and Pathogenesis , 1998, Infection and Immunity.

[13]  F. Kunst,et al.  Chromosomal location of mutations affecting sucrose metabolism in Bacillus subtilis Marburg , 2004, Molecular and General Genetics MGG.

[14]  W. Wackernagel,et al.  Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant. , 1995, Gene.

[15]  B. Ames,et al.  Positive selection for loss of tetracycline resistance , 1980, Journal of bacteriology.

[16]  B. Dujon,et al.  Purification and characterization of the in vitro activity of I-Sce I, a novel and highly specific endonuclease encoded by a group I intron. , 1990, Nucleic acids research.

[17]  E. Raleigh,et al.  A versatile element for gene addition in bacterial chromosomes , 2011, Nucleic acids research.

[18]  Jay D. Keasling,et al.  A Propionate-Inducible Expression System for Enteric Bacteria , 2005, Applied and Environmental Microbiology.

[19]  D. le Coq,et al.  Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria , 1985, Journal of bacteriology.

[20]  J. Keasling,et al.  Heterologous protein production in Escherichia coli using the propionate-inducible pPro system by conventional and auto-induction methods. , 2008, Protein expression and purification.

[21]  H. Schweizer,et al.  A novel phosphate-regulated expression vector in Escherichia coli. , 1990, Gene.

[22]  A. Spormann,et al.  Dissimilatory iron reduction in Escherichia coli: identification of CymA of Shewanella oneidensis and NapC of E. coli as ferric reductases , 2008, Molecular microbiology.

[23]  D. Court,et al.  An efficient recombination system for chromosome engineering in Escherichia coli. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[24]  N. L. Craig.  Transposon Tn7. , 1996, Current topics in microbiology and immunology.

[25]  K. Hammer,et al.  The Sequence of Spacers between the Consensus Sequences Modulates the Strength of Prokaryotic Promoters , 1998, Applied and Environmental Microbiology.

[26]  F. Blattner,et al.  Markerless gene replacement in Escherichia coli stimulated by a double-strand break in the chromosome. , 1999, Nucleic acids research.

[27]  M. Seeger,et al.  New alkane-responsive expression vectors for Escherichia coli and pseudomonas. , 2001, Plasmid.

[28]  K. Murphy,et al.  Use of Bacteriophage λ Recombination Functions To Promote Gene Replacement in Escherichia coli , 1998, Journal of bacteriology.

[29]  M. Reuss,et al.  High-cell-density fermentation for production of L-N-carbamoylase using an expression system based on the Escherichia coli rhaBAD promoter. , 2001, Biotechnology and bioengineering.

[30]  Selection for loss of tetracycline resistance by Escherichia coli. , 1981, Journal of bacteriology.

[31]  M. De Mey,et al.  Promoter knock-in: a novel rational method for the fine tuning of genes , 2010, BMC biotechnology.

[32]  Nancy A. Jenkins,et al.  Simple and highly efficient BAC recombineering using galK selection , 2005, Nucleic acids research.

[33]  B. Wanner,et al.  One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[34]  R. Heermann,et al.  Microbial Cell Factories Simple Generation of Site-directed Point Mutations in the Escherichia Coli Chromosome Using Red ® /et ® Recombination , 2022 .

[35]  N. Craig,et al.  Fast, easy and efficient: site-specific insertion of transgenes into Enterobacterial chromosomes using Tn7 without need for selection of the insertion event , 2006, BMC microbiology.

[36]  M. Elowitz,et al.  Programming gene expression with combinatorial promoters , 2007, Molecular systems biology.

[37]  J. Altenbuchner,et al.  Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations , 2008, BMC biotechnology.

[38]  Xin Yan,et al.  Cre/lox System and PCR-Based Genome Engineering in Bacillus subtilis , 2008, Applied and Environmental Microbiology.

[39]  H. Schweizer,et al.  A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. , 1998, Gene.

[40]  Jo Maertens,et al.  Construction and model-based analysis of a promoter library for E. coli: an indispensable tool for metabolic engineering , 2007, BMC biotechnology.

[41]  Frank Buchholz,et al.  A new logic for DNA engineering using recombination in Escherichia coli , 1998, Nature Genetics.

[42]  J. Keasling,et al.  Propionate‐regulated high‐yield protein production in Escherichia coli , 2006, Biotechnology and bioengineering.

[43]  G. Stephanopoulos,et al.  Tuning genetic control through promoter engineering. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[44]  Guy Plunkett,et al.  Engineering a reduced Escherichia coli genome. , 2002, Genome research.