Effects of Growth Regulators on Adventitious Root Growth and Eleutherosides and Chlorogenic Acid Accumulation in Air Lift Bioreactor Cultures of Eleutherococcus koreanum

The influence of different growth regulators on biomass of adventitious roots and secondary metabolites accumulation in bioreactor cultures of Eleutherococcus koreanum was studied. The maximum growth of adventitious roots was observed in the presence of IBA (6.7 g DW/L). However the productivity of eleutheroside E was not significantly different among IBA levels. High level of thidiazuron (TDZ) efficiently increased both root growth and secondary metabolites production. Especially when TDZ was combined with IBA, the highest eleutheroside E accumulation (DW) was observed. When the same TDZ level was treated in combination with IBA, both chlorogrnic acid and eleutheroside B were accumulated to maximum level. In contrast, zeatin remarkably suppressed the accumulation of both eleutheroside B and chlorogenic acid.

[1]  Gokare A. Ravishankar,et al.  Polyamine and methyl jasmonate-influenced enhancement of betalaine production in hairy root cultures of Beta vulgaris grown in a bubble column reactor and studies on efflux of pigments , 2004 .

[2]  P. Saxena,et al.  In vitro-grown roots: a superior explant for prolific shoot regeneration of St. John's wort (Hypericum perforatum L. cv ‘New Stem’) in a temporary immersion bioreactor , 2003 .

[3]  Wenyuan Gao,et al.  Jasmonic acid improves ginsenoside accumulation in adventitious root culture of Panax ginseng C.A. Meyer , 2002 .

[4]  H. Vaikousi,et al.  Characterization of pullulan produced from beet molasses by Aureobasidium pullulans in a stirred tank reactor under varying agitation , 2002 .

[5]  A. Gravot,et al.  Production of plant secondary metabolites: a historical perspective , 2001 .

[6]  H. Bais,et al.  Influence of polyamines on growth and formation of secondary metabolites in hairy root cultures of Beta vulgaris and Tagetes patula , 2000, Acta Physiologiae Plantarum.

[7]  S. H. Son,et al.  Large-scale growth and taxane production in cell cultures of Taxus cuspidata (Japanese yew) using a novel bioreactor , 2000, Plant Cell Reports.

[8]  Deok-Chun Yang,et al.  Rapid propagation of Eleutherococcus senticosus via direct somatic embryogenesis from explants of seedlings , 1999, Plant Cell, Tissue and Organ Culture.

[9]  T. Nohara,et al.  A lupane-triterpene glycoside from leaves of two Acanthopanax , 1998 .

[10]  J. Kim,et al.  Somatic embryogenesis and plant regeneration from suspension cultures of Acanthopanax koreanum Nakai , 1997, Plant Cell Reports.

[11]  H. Kang,et al.  Suppression of interleukin-1 and tumor necrosis factor-alpha production by acanthoic acid, (-)-pimara-9(11),15-dien-19-oic acid, and it antifibrotic effects in vivo. , 1996, Cellular immunology.

[12]  K. Hostettmann,et al.  The isolation of eleutherococcus senticosus constituents by centrifugal partition chromatography and their quantitative determination by high performance liquid chromatography , 1991 .

[13]  J. Takeda.  Light-Induced Synthesis of Anthocyanin in Carrot Cells in Suspension I. THE FACTORS AFFECTING ANTHOCYANIN PRODUCTION , 1988 .

[14]  M. Shuler,et al.  Environmental parameters influencing phenolics production by batch cultures of Nicotiana tabacum , 1984, Biotechnology and bioengineering.

[15]  F. Skoog,et al.  A revised medium for rapid growth and bio assays with tobacco tissue cultures , 1962 .

[16]  M. S. Narayan,et al.  Interplay of growth regulators during solid-state and liquid-state batch cultivation of anthocyanin producing cell line of Daucus carota , 2005 .