Hybridization in the Diploid-Tetraploid Treefrogs Hyla chrysoscelis and Hyla versicolor

The first cytologically confirmed natural hybrids between the diploid-tetraploid gray treefrogs, Hyla chrysoscelis and H. versicolor, were two triploid males initially detected by their distinctive advertisement calls. Analyses of the pulserepetition rate and shape of the advertisement calls of these triploid hybrids suggest that one of the two previously reported putative hybrids was a hybrid and the other male was a H. chrysoscelis. The calls of the field-recorded hybrids were intermediate in pulse rate in comparison to the parental species and significantly different in structure than were the calls of laboratory-reared hybrids described by Mable and Bogart (1991). Mated pairs were collected in six syntopic populations in south-central Missouri. Flow cytometric identifications revealed that five (about 6.7%) of the 75 pairs in two of these ponds were mismated; these five mismatings represented about 3% of all 181 pairs examined from all Missouri populations. The rarity of sexually mature hybrids, despite a fairly high frequency of mismatings in at least two localities, indicates severe selection against hybrids. In four of the five mismated pairs, the female was H. versicolor. Analyses of mtDNA sequences showed that the female parent of the two cytologically confirmed natural hybrids was also H. versicolor.

[1]  A Krishan,et al.  Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining , 1975, The Journal of cell biology.

[2]  H. Gerhardt,et al.  Natural Hybrids between Hyla cinerea and Hyla gratiosa: Morphology, Vocalization and Electrophoretic Analysis , 1980 .

[3]  Distribution and Call Parameters of Hyla chrysoscelis and Hyla versicolor in Michigan , 1979 .

[4]  C. Johnson.  Genetic Incompatibility in the Call Races of Hyla versicolor Le Conte in Texas , 1959 .

[5]  K. Mullis,et al.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. , 1985, Science.

[6]  Y. Yoneyama.  [The nucleotide sequences of the heavy and light strand replication origins of the Rana catesbeiana mitochondrial genome]. , 1987, Nihon Ika Daigaku zasshi.

[7]  R. Zweifel.  Distribution and mating call of the treefrog, Hyla chrysoscelis , at the northeastern edge of its range , 1970 .

[8]  J. Bogart,et al.  CALL ANALYSIS OF TRIPLOID HYBRIDS RESULTING FROM DIPLOID-TETRAPLOID SPECIES CROSSES OF HYLID TREE FROGS , 1991 .

[9]  J C Stout,et al.  Interstitial hybridization sites of the (TTAGGG)n telomeric sequence on the chromosomes of some North American hylid frogs. , 1992, Cytogenetics and cell genetics.

[10]  J. Bogart,et al.  Diploid-polyploid cryptic species pairs: a possible clue to evolution by polyploidization in anuran amphibians. , 1972, Cytogenetics.

[11]  D. B. Ralin.  EVOLUTIONARY ASPECTS OF MATING CALL VARIATION IN A DIPLOID‐TETRAPLOID SPECIES COMPLEX OF TREEFROGS (ANURA) , 1977, Evolution; international journal of organic evolution.

[12]  R. Butlin.  Speciation by reinforcement. , 1987, Trends in ecology & evolution.

[13]  A. Meyer,et al.  Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. , 1989, Proceedings of the National Academy of Sciences of the United States of America.

[14]  S. Guttman,et al.  Effects of Twenty Years of Hybridization in a Disturbed Habitat on Hyla cinerea and Hyla gratiosa , 1986 .

[15]  J. Avise,et al.  Directional introgression of mitochondrial DNA in a hybrid population of tree frogs: The influence of mating behavior. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[16]  A. O. Wasserman.  Polyploidy in the Conitnon Tree Toad Hyla versicolor Le Conte , 1970, Science.