Optical sensing method to analyze germination rate of Capsicum annum seeds treated with growth-promoting chemical compounds using optical coherence tomography

Abstract. Seed germination rate differs based on chemical treatments, and nondestructive measurements of germination rate have become an essential requirement in the field of agriculture. Seed scientists and other biologists are interested in optical sensing technologies-based biological discoveries due to nondestructive detection capability. Optical coherence tomography (OCT) has recently emerged as a powerful method for biological and plant material discoveries. We report an extended application of OCT by monitoring the germination rate acceleration of chemically primed seeds. To validate the versatility of the method, Capsicum annum seeds were primed using three chemical compounds: sterile distilled water (SDW), butandiol, and 1-hexadecene. Monitoring was performed using a 1310-nm swept source OCT system. The results confirmed more rapid morphological variations in the seeds treated with 1-hexadecene medium than the seeds treated with SDW and butandiol within 8 consecutive days. In addition, fresh weight measurements (gold standard) of seeds were monitored for 15 days, and the obtained results were correlated with the OCT results. Thus, such a method can be used in various agricultural fields, and OCT shows potential as a rigorous sensing method for selecting the optimal plant growth-promoting chemical compounds rapidly, when compared with the gold standard methods.

[1]  Sang-Min Kim,et al.  Destruction of Cucumber green mottle mosaic virus by heat treatment and rapid detection of virus inactivation by RT-PCR. , 2003, Molecules and cells.

[2]  R. Kuranov,et al.  Study of the Morphological and Functional State of Higher Plant Tissues by Optical Coherence Microscopy and Optical Coherence Tomography , 2005, Russian Journal of Plant Physiology.

[3]  J. Medford,et al.  In vivo three‐dimensional imaging of plants with optical coherence microscopy , 2002, Journal of microscopy.

[4]  Jeehyun Kim,et al.  Application of optical coherence tomography to detect Cucumber green mottle mosaic virus (CGMMV) infected cucumber seed , 2012, Horticulture, Environment, and Biotechnology.

[5]  Jeehyun Kim,et al.  In Vivo Monitoring on Growth and Spread of Gray Leaf Spot Disease in Capsicum annuum Leaf Using Spectral Domain Optical Coherence Tomography , 2016 .

[6]  Maya Kumari,et al.  Seed Priming Mediated Germination Improvement and Tolerance to Subsequent Exposure to Cold and Salt Stress in Capsicum , 2011 .

[7]  Sushma Tamta,et al.  Effect of pre-sowing treatments on seed germination in Quercus serrata Thunb. and Quercus semecarpifolia Sm. , 2013 .

[8]  D. Vwioko,et al.  Effects of spent lubricating oil on the growth of Capsicum annum L. and Lycopersicon esculentum Miller. , 1995, Environmental pollution.

[9]  Jeehyun Kim,et al.  Evaluation of the usefulness of three-dimensional optical coherence tomography in a guinea pig model of endolymphatic hydrops induced by surgical obliteration of the endolymphatic duct , 2015, Journal of biomedical optics.

[10]  Hobin Kang,et al.  Automated assessment of the remineralization of artificial enamel lesions with polarization-sensitive optical coherence tomography. , 2014, Biomedical optics express.

[11]  A. K. Biswas,et al.  Effect of distillery effluent on seed germination in some vegetable crops. , 2002, Bioresource technology.

[12]  S. Ghim,et al.  Determinants of Plant Growth-promoting Ochrobactrum lupini KUDC1013 Involved in Induction of Systemic Resistance against Pectobacterium carotovorum subsp. carotovorum in Tobacco Leaves , 2013, The plant pathology journal.

[13]  Jeehyun Kim,et al.  Quantitative assessment of touch-screen panel by nondestructive inspection with three-dimensional real-time display optical coherence tomography , 2015 .

[14]  Heeyoung Jung,et al.  Optical Sensing Method for Screening Disease in Melon Seeds by Using Optical Coherence Tomography , 2011, Sensors.

[15]  Jeehyun Kim,et al.  The Application of Optical Coherence Tomography in the Diagnosis of Marssonina Blotch in Apple Leaves , 2012 .

[16]  J. Fujimoto,et al.  Ultrahigh-resolution ophthalmic optical coherence tomography , 2001, Nature Medicine.

[17]  S. Yun,et al.  In vivo high-resolution video-rate spectral-domain optical coherence tomography of the human retina and optic nerve. , 2004, Optics express.

[18]  Jeehyun Kim,et al.  Handheld Optical Coherence Tomography Scanner for Primary Care Diagnostics , 2011, IEEE Transactions on Biomedical Engineering.

[19]  C. M. Karssen,et al.  Gibberellins regulate seed germination in tomato by endosperm weakening: a study with gibberellin-deficient mutants , 1987, Planta.

[20]  Daniel Fried,et al.  Use of 2D images of depth and integrated reflectivity to represent the severity of demineralization in cross-polarization optical coherence tomography. , 2015, Journal of biophotonics.

[21]  J. Duker,et al.  Three-dimensional retinal imaging with high-speed ultrahigh-resolution optical coherence tomography. , 2005, Ophthalmology.

[22]  Ruchire Eranga Wijesinghe,et al.  Decalcification using ethylenediaminetetraacetic acid for clear microstructure imaging of cochlea through optical coherence tomography , 2016, Journal of biomedical optics.

[23]  G. Madhu,et al.  Effect of pre-sowing treatment with permanent magnetic field on germination and growth of chilli (Capsicum annum. L.). , 2009 .

[24]  Pil Un Kim,et al.  Optical Inspection and Morphological Analysis of Diospyros kaki Plant Leaves for the Detection of Circular Leaf Spot Disease , 2016, Sensors.

[25]  Ping Shum,et al.  Dual spectrometer system with spectral compounding for 1-μm optical coherence tomography in vivo. , 2014, Optics letters.

[26]  B. Cobb,et al.  Accelerated germination of pepper seed by priming with salt solutions and water , 1991 .

[27]  M. Farag,et al.  Bacterial volatiles promote growth in Arabidopsis , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[28]  Jeehyun Kim,et al.  In vivo imaging of middle-ear and inner-ear microstructures of a mouse guided by SD-OCT combined with a surgical microscope. , 2014, Optics express.

[29]  Alberto Pardossi,et al.  Seedling Root Morphology and Shoot Growth after Seed Priming or Pregermination of Bell Pepper , 1992 .

[30]  David D. Sampson,et al.  Optical coherence tomography as a novel tool for non-destructive measurement of the hull thickness of lupin seeds , 2004 .

[31]  Grigory V. Gelikonov,et al.  In vivo monitoring of seeds and plant-tissue water absorption using optical coherence tomography and optical coherence microscopy , 2004, SPIE BiOS.

[32]  Bart Nicolai,et al.  Optical coherence tomography visualizes microstructure of apple peel , 2013 .