Group II intron RNA catalysis of progressive nucleotide insertion: a model for RNA editing.

The self-splicing bl1 intron lariat from mitochondria of Saccharomyces cerevisiae catalyzed the insertion of nucleotidyl monomers derived from the 3' end of a donor RNA into an acceptor RNA in a 3' to 5' direction in vitro. In this catalyzed reaction, the site specificity provided by intermolecular base pair interactions, the formation of chimeric intermediates, the polarity of the nucleotidyl insertion, and its reversibility all resemble such properties in previously proposed models of RNA editing in kinetoplastid mitochondria. These results suggest that RNA editing occurs by way of a concerted, two-step transesterification mechanism and that RNA splicing and RNA editing might be prebiotically related mechanisms; possibly, both evolved from a primordial demand for self-replication.

[1]  O. Uhlenbeck,et al.  3'-Phosphatase activity in T4 polynucleotide kinase. , 1977, Biochemistry.

[2]  T. Cech,et al.  A model for the RNA-catalyzed replication of RNA. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[3]  T. Cech,et al.  The intervening sequence RNA of Tetrahymena is an enzyme. , 1986, Science.

[4]  R. Schweyen,et al.  Self-splicing of group II introns in vitro: Mapping of the branch point and mutational inhibition of lariat formation , 1986, Cell.

[5]  F. Michel,et al.  Multiple exon-binding sites in class II self-splicing introns , 1987, Cell.

[6]  T. Cech The chemistry of self-splicing RNA and RNA enzymes. , 1987, Science.

[7]  Manfred W. Müller,et al.  Self-splicing of group II introns in vitro: Lariat formation and 3′ splice site selection in mutant RNAs , 1987, Cell.

[8]  T. Cech,et al.  RNA as an RNA polymerase: net elongation of an RNA primer catalyzed by the Tetrahymena ribozyme. , 1988, Science.

[9]  M. Uhlén,et al.  Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. , 1989, Nucleic acids research.

[10]  G. F. Joyce RNA evolution and the origins of life , 1989, Nature.

[11]  Jennifer A. Doudna,et al.  RNA-catalysed synthesis of complementary-strand RNA , 1989, Nature.

[12]  Evidence of the mid-latitude impact of Antarctic ozone depletion , 1989, Nature.

[13]  J. Shaw,et al.  RNA editing and the mitochondrial cryptogenes of kinetoplastid protozoa , 1989, Cell.

[14]  M. Billeter,et al.  Sometimes an editor makes sense , 1990, Nature.

[15]  M. Mörl,et al.  Group II intron RNA-catalyzed recombination of RNA in vitro. , 1990, Nucleic acids research.

[16]  J. Feagin RNA editing in kinetoplastid mitochondria. , 1990, The Journal of biological chemistry.

[17]  A. Weiner,et al.  RNA editing: Guided but not templated? , 1990, Cell.

[18]  M. Mörl,et al.  Integration of group II intron bl1 into a foreign RNA by reversal of the self-splicing reaction in vitro , 1990, Cell.

[19]  R. Schweyen,et al.  Reverse self-splicing of group II intron RNAs in vitro , 1990, Nature.

[20]  L. Simpson,et al.  Chimeric gRNA-mRNA molecules with oligo(U) tails covalently linked at sites of RNA editing suggest that U addition occurs by transesterification , 1991, Cell.

[21]  T. Cech,et al.  RNA editing: World's smallest introns? , 1991, Cell.

[22]  K. Stuart RNA editing in mitochondrial mRNA of trypanosomatids. , 1991, Trends in biochemical sciences.

[23]  M. Hetzer,et al.  Fate of the junction phosphate in alternating forward and reverse self-splicing reactions of group II intron RNA. , 1991, Journal of molecular biology.

[24]  B. Sollner-Webb Guides to experiments , 1992, Nature.

[25]  S. Hajduk,et al.  Kinetoplastid RNA editing: In vitro formation of cytochrome b gRNA-mRNA chimeras from synthetic substrate RNAs , 1992, Cell.

[26]  K. Stuart,et al.  In vitro guide RNA/mRNA chimaera formation in Trypanosoma bruceiRNA editing , 1992, Nature.