Chapter 10 – Application of Padlock and Selector Probes in Molecular Medicine

Padlock and selector probes are versatile tools that have been used and developed as great alternatives to molecular methods for the detection of nucleic acid sequences in the context of medicine. Their application has been proven to be useful in different areas from targeted sequencing, genotyping, and molecular diagnostics to the development of novel biosensors and techniques. This chapter describes the molecular functioning of these probes, in combination with DNA ligase-assisted specific circularization and isothermal rolling circle amplification, their properties, and their main applications in the field of medicine to date. It shows the potential of these approaches for developing assays that can be integrated into assays or instruments that will enable and improve access to information valuable for molecular medicine research and for improving medical diagnostics.

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