The Promoter for the Ovine Follicle-Stimulating Hormone-β Gene (FSHβ) Confers FSHβ-Like Expression on Luciferase in Transgenic Mice: Regulatory Studies in Vivo and in Vitro.

Transgenic mice harboring the ovine FSHβ (oFSHβ) promoter plus first intron (from −4741 to +759 bp) linked to a luciferase reporter gene (oFSHβLuc) were generated to determine whether this promoter can direct tissue-specific expression in vivo and serve as a model for studying hormonal regulation of the FSHβ gene. Of six lines of transgenic mice analyzed, luciferase was detected uniquely in the pituitaries of five of them. Pituitary luciferase activity was decreased 51–99% by chronic GnRH treatment (Lupron depot). Orchidectomy caused a 2- to 8-fold increase, and ovariectomy caused a 2- to 27-fold increase in pituitary luciferase activity. Furthermore, pituitary luciferase expression was consistently higher on estrus than on diestrus (3- to 20-fold). These data strongly suggested that the transgene was expressed specifically in pituitary gonadotropes and regulated in the same way as the endogenous mouse FSHβ gene. Using primary pituitary cell cultures prepared from these transgenic mice, basal luciferase e...

[1]  H. Huang,et al.  A novel role for bone morphogenetic proteins in the synthesis of follicle-stimulating hormone. , 2001, Endocrinology.

[2]  B. Strahl,et al.  Transcriptional Regulation of the Ovine Follicle-Stimulating Hormone-β Gene by Activin and Gonadotropin-Releasing Hormone (GnRH): Involvement of Two Proximal Activator Protein-1 Sites for GnRH Stimulation. , 2001, Endocrinology.

[3]  M. Low,et al.  LbetaT2 gonadotroph cells secrete follicle stimulating hormone (FSH) in response to active A. , 1999, The Journal of endocrinology.

[4]  M. Avilés,et al.  Hypokalemia alters sex hormone and gonadotropin levels: evidence that FSH may be required for luteinization. , 1998, American journal of physiology. Endocrinology and metabolism.

[5]  B. Strahl,et al.  Transcriptional Activation of the Ovine Follicle-Stimulating Hormone β-Subunit Gene by Gonadotropin-Releasing Hormone: Involvement of Two Activating Protein-1-Binding Sites and Protein Kinase C* *This work was supported by the North Carolina State University Agricultural Research Service, NICHD Gran , 1998, Endocrinology.

[6]  B. Strahl,et al.  Transcriptional Activation of the Ovine Follicle-Stimulating Hormone β-Subunit Gene by Gonadotropin-Releasing Hormone: Involvement of Two Activating Protein-1-Binding Sites and Protein Kinase C* *This work was supported by the North Carolina State University Agricultural Research Service, NICHD Gran , 1998, Endocrinology.

[7]  M. Matzuk,et al.  Follicle stimulating hormone is required for ovarian follicle maturation but not male fertility , 1997, Nature Genetics.

[8]  W. L. Miller,et al.  Transcriptional repression of the ovine follicle-stimulating hormone-beta gene by 17 beta-estradiol. , 1996, Endocrinology.

[9]  D. Edwards,et al.  The 5'-flanking region of the ovine follicle-stimulating hormone-beta gene contains six progesterone response elements: three proximal elements are sufficient to increase transcription in the presence of progesterone. , 1995, Endocrinology.

[10]  Fuping Zhang,et al.  The FSH β-subunit promoter directs the expression of Herpes simplex virus type 1 thymidine kinase to the testis of transgenic mice , 1993, Molecular and Cellular Endocrinology.

[11]  M. Low,et al.  Gonadal steroid hormone regulation of human and mouse follicle stimulating hormone beta-subunit gene expression in vivo. , 1993, Molecular endocrinology.

[12]  M. Low,et al.  Gonadotrope-specific expression of the human follicle-stimulating hormone beta-subunit gene in pituitaries of transgenic mice. , 1992, Molecular endocrinology.

[13]  D. Haisenleder,et al.  A pulsatile gonadotropin-releasing hormone stimulus is required to increase transcription of the gonadotropin subunit genes: evidence for differential regulation of transcription by pulse frequency in vivo. , 1991, Endocrinology.

[14]  P. Leung,et al.  Androgens positively regulate follicle-stimulating hormone beta-subunit mRNA levels in rat pituitary cells. , 1990, Molecular endocrinology.

[15]  R. Clayton,et al.  Comparison of the pretranslational regulation of FSH synthesis by gonadal steroids in rats and mice. , 1990, Journal of molecular endocrinology.

[16]  R. Carroll,et al.  Inhibin, activin, and follistatin: regulation of follicle-stimulating hormone messenger ribonucleic acid levels. , 1989, Molecular endocrinology.

[17]  R. Clayton,et al.  Gonadotropin-releasing hormone regulates follicle-stimulating hormone beta-subunit gene expression in the male rat. , 1989, Endocrinology.

[18]  W. Chin,et al.  Divergent effects of estradiol on gonadotropin gene transcription in pituitary fragments. , 1989, Molecular endocrinology.

[19]  J. Clements,et al.  Regulation of follicle-stimulating hormone β and common α-subunit messenger ribonucleic acid by gonadotropin-releasing hormone and estrogen in the sheep pituitary , 1989 .

[20]  R. Maurer,et al.  Follicle-stimulating hormone beta subunit messenger ribonucleic acid concentrations during the rat estrous cycle. , 1988, Endocrinology.

[21]  A. Milsted,et al.  17β-Estradiol and Progesterone Inhibit Transcription of the Genes Encoding the Subunits of Ovine Follicle-Stimulating Hormone , 1988 .

[22]  T. Badger,et al.  Sex steroid hormone regulation of follicle-stimulating hormone subunit messenger ribonucleic acid (mRNA) levels in the rat. , 1987, The Journal of clinical investigation.

[23]  I. Huhtaniemi,et al.  Testicular gonadotrophins and their receptors in human cryptorchidism as revealed by immunohistochemistry and radioreceptor assay. , 1986, Acta endocrinologica.

[24]  I. Huhtaniemi,et al.  Localization of luteinizing hormone, follicle-stimulating hormone, prolactin, and their receptors in human and rat testis using immunohistochemistry and radioreceptor assay. , 1983, The Journal of clinical endocrinology and metabolism.

[25]  J. Drouin,et al.  Interactions between 17 beta-estradiol and progesterone in the control of luteinizing hormone and follicle-stimulating hormone release in rat anterior pituitary cells in culture. , 1981, Endocrinology.

[26]  W. L. Miller,et al.  Effects of estradiol-17 beta on basal and luteinizing hormone releasing hormone-induced secretion of luteinizing hormone and follicle stimulating hormone by ovine pituitary cell culture. , 1980, Biology of reproduction.

[27]  A. Parlow,et al.  Alterations in serum FSH-LH ratios in relation to the estrous cycle, pseudopregnancy, and gonadectomy in the mouse. , 1972, Endocrinology.