Periosteal Progenitor Cell Fate in Segmental Cortical Bone Graft Transplantations: Implications for Functional Tissue Engineering

A murine segmental femoral bone graft model was used to show the essential role of donor periosteal progenitor cells in bone graft healing. Transplantation of live bone graft harvested from Rosa 26A mice showed that ∼70% of osteogenesis on the graft was attributed to the expansion and differentiation of donor periosteal progenitor cells. Furthermore, engraftment of BMP‐2‐producing bone marrow stromal cells on nonvital allografts showed marked increases in cortical graft incorporation and neovascularization, suggesting that gene‐enhanced, tissue engineered functional periosteum may improve allograft incorporation and repair.

[1]  Hiromu Ito,et al.  Remodeling of cortical bone allografts mediated by adherent rAAV-RANKL and VEGF gene therapy , 2005, Nature Medicine.

[2]  E. Schwarz,et al.  A novel murine segmental femoral graft model , 2004, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[3]  W. R. Taylor,et al.  Quantitative microcomputed tomography analysis of collateral vessel development after ischemic injury. , 2004, American journal of physiology. Heart and circulatory physiology.

[4]  D. Vanel,et al.  Imaging of vascularized fibula autograft placed inside a massive allograft in reconstruction of lower limb bone tumors. , 2004, AJR. American journal of roentgenology.

[5]  J. K. Chen,et al.  Ex vivo gene therapy in autologous bone marrow stromal stem cells for tissue-engineered maxillofacial bone regeneration , 2003, Gene Therapy.

[6]  R E Guldberg,et al.  Analyzing bone, blood vessels, and biomaterials with microcomputed tomography. , 2003, IEEE engineering in medicine and biology magazine : the quarterly magazine of the Engineering in Medicine & Biology Society.

[7]  R. Midura,et al.  Practical Modeling Concepts for Connective Tissue Stem Cell and Progenitor Compartment Kinetics , 2003, Journal of biomedicine & biotechnology.

[8]  P. Campbell,et al.  Effect of Regional Gene Therapy with Bone Morphogenetic Protein-2-Producing Bone Marrow Cells on Spinal Fusion in Rats , 2003, The Journal of bone and joint surgery. American volume.

[9]  L. Bonewald,et al.  MLO‐Y4 Osteocyte‐Like Cells Support Osteoclast Formation and Activation , 2002, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[10]  S. Karaoğlu,et al.  Experimental repair of segmental bone defects in rabbits by demineralized allograft covered by free autogenous periosteum. , 2002, Injury.

[11]  E. Schwarz,et al.  Cyclooxygenase-2 regulates mesenchymal cell differentiation into the osteoblast lineage and is critically involved in bone repair. , 2002, The Journal of clinical investigation.

[12]  W. Enneking,et al.  Retrieved Human Allografts: A Clinicopathological Study , 2001, The Journal of bone and joint surgery. American volume.

[13]  R. Müller,et al.  Engineered human mesenchymal stem cells: a novel platform for skeletal cell mediated gene therapy , 2001, The journal of gene medicine.

[14]  R. Müller,et al.  Exogenously regulated stem cell-mediated gene therapy for bone regeneration. , 2001, Molecular therapy : the journal of the American Society of Gene Therapy.

[15]  E. Schwarz,et al.  NF-κB Regulates VCAM-1 Expression on Fibroblast-Like Synoviocytes1 , 2000, The Journal of Immunology.

[16]  G. Muschler,et al.  Bone graft materials. An overview of the basic science. , 2000, Clinical orthopaedics and related research.

[17]  D. Davy.  Biomechanical issues in bone transplantation. , 1999, The Orthopedic clinics of North America.

[18]  H. Mankin,et al.  Bone banking. Update on methods and materials. , 1999, The Orthopedic clinics of North America.

[19]  Theodore Miclau,et al.  Does adult fracture repair recapitulate embryonic skeletal formation? , 1999, Mechanisms of Development.

[20]  P. Rüegsegger,et al.  Direct Three‐Dimensional Morphometric Analysis of Human Cancellous Bone: Microstructural Data from Spine, Femur, Iliac Crest, and Calcaneus , 1999, Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research.

[21]  A Daluiski,et al.  The effect of regional gene therapy with bone morphogenetic protein-2-producing bone-marrow cells on the repair of segmental femoral defects in rats. , 1999, The Journal of bone and joint surgery. American volume.

[22]  T A Einhorn,et al.  The cell and molecular biology of fracture healing. , 1998, Clinical orthopaedics and related research.

[23]  D. Hu,et al.  Common Molecular Pathways in Skeletal Morphogenesis and Repair , 1998, Annals of the New York Academy of Sciences.

[24]  B. Masri,et al.  Biology of allografting. , 1998, The Orthopedic clinics of North America.

[25]  Giuseppe M Peretti,et al.  Recapitulation of signals regulating embryonic bone formation during postnatal growth and in fracture repair , 1998, Mechanisms of Development.

[26]  L A Herzenberg,et al.  Disruption of overlapping transcripts in the ROSA beta geo 26 gene trap strain leads to widespread expression of beta-galactosidase in mouse embryos and hematopoietic cells. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[27]  D. Davy,et al.  Critical Biological Determinants of Incorporation of Non-Vascularized Cortical Bone Grafts. Quantification of a Complex Process and Structure*† , 1997, The Journal of bone and joint surgery. American volume.

[28]  P. Prendergast.  Biomechanics in orthopaedic trauma , 1996 .

[29]  V. Goldberg,et al.  Factors Affecting Bone Graft Incorporation , 1996, Clinical orthopaedics and related research.

[30]  V. Goldberg,et al.  The biology of bone grafts. , 1993, Seminars in arthroplasty.

[31]  W. Enneking,et al.  Observations on massive retrieved human allografts. , 1991, The Journal of bone and joint surgery. American volume.

[32]  M. Gebhardt,et al.  Fractures of allografts. Frequency, treatment, and end-results. , 1990, The Journal of bone and joint surgery. American volume.

[33]  Włodarski Kh.  Normal and heterotopic periosteum. , 1989 .

[34]  M. Gebhardt,et al.  Infection in bone allografts. Incidence, nature, and treatment. , 1988, The Journal of bone and joint surgery. American volume.

[35]  H. Burchardt.  Biology of bone transplantation. , 1987, The Orthopedic clinics of North America.

[36]  H. Burchardt.  The biology of bone graft repair. , 1983, Clinical orthopaedics and related research.

[37]  Dietmar W Hutmacher,et al.  Periosteal cells in bone tissue engineering. , 2003, Tissue engineering.

[38]  G. Szczęsny.  Molecular aspects of bone healing and remodeling. , 2002, Polish journal of pathology : official journal of the Polish Society of Pathologists.

[39]  W. Enneking,et al.  Biomechanical evaluation of retrieved massive allografts: preliminary results. , 2001, Biomedical sciences instrumentation.

[40]  J. Aubin,et al.  Advances in the osteoblast lineage. , 1998, Biochemistry and cell biology = Biochimie et biologie cellulaire.

[41]  TOR Hildebrand,et al.  Quantification of Bone Microarchitecture with the Structure Model Index. , 1997, Computer methods in biomechanics and biomedical engineering.

[42]  Jian Yin Liu,et al.  Use of revascularized periosteal allografts for repairing bony defects: An experimental study , 1994, Microsurgery.

[43]  K. Włodarski.  Normal and heterotopic periosteum. , 1989, Clinical orthopaedics and related research.

[44]  I. Dick,et al.  The transplantation of bone. , 1957, Journal of the Royal College of Surgeons of Edinburgh.