Optimization of Multiplex PCRs

The development of the polymerase chain reaction (PCR) has enabled rapid and efficient analysis of specific DNA sequences (Mullis and Faloona, 1987). Most PCR strategies are designed to amplify one or more target sequences with a single set of oligonucleotide primers. However, many experimental approaches require the analysis of a variety of DNA sequences, which necessitates that multiple PCRs be performed on the same or related DNA templates. Considerable savings of time and effort can be achieved by simultaneously amplifying multiple sequences in a single reaction, a process referred to as multiplex PCR (Chamberlain et al., 1988, Chamberlain et al., 1989).

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