Novel synthesis and biochemical properties of an [125I]-labeled photoaffinity probe for thromboxane A2/prostaglandin H2 receptors.

We have described an efficient synthesis of an 125 -labeled photoaffinity probe of high affinity for human platelet TXA 2 /PGH 2 receptors. The high affinity of this probe and its irreversible incorporation into the TXA 2 receptor should make it a useful tool for the study of this receptor. This synthesis was accomplished via an exchange of the 127 I isotope with a trimethyltin group in the presence of an azide group followed by an electrophilic destannylation using [ 125 I]ICl generated with chloramine-T