Fibrillin Containing Elastic Microfibrils Support Platelet Adhesion under Dynamic Shear Conditions

Summary The vascular subendothelium contains macromolecular structures called microfibrils. Type VI collagen is one protein found in microfibrils that supports platelet adhesion and aggregation and we have previously evaluated the roles of platelet receptors and vWf involved in these processes under physiological shear conditions. Here we investigate the ability of fibrillin containing elastic microfibrils to support mural thrombus formation. Our results show that elastic microfibril surfaces support platelet adhesion under low shear conditions at a level similar to collagen VI tetramers. However, the degree of aggregation on the elastic microfibril surface is much higher. Both adhesion and aggregation were shown to be mediated by the GPIIb-IIIa platelet receptor. Elastic microfibrils do not support the formation of mural thrombi under high shear conditions. These results suggest roles for both collagen VI and fibrillin containing elastic microfibrils in modulating the platelet response to blood vessel injury.

[1]  R. Glanville,et al.  Morphological relationships of von Willebrand factor, type VI collagen, and fibrillin in human vascular subendothelium. , 1996, The American journal of pathology.

[2]  J. Moake,et al.  Platelet adhesion and aggregation on human type VI collagen surfaces under physiological flow conditions. , 1995, Blood.

[3]  J. Sixma,et al.  Platelet adhesion to collagen types I through VIII under conditions of stasis and flow is mediated by GPIa/IIa (alpha 2 beta 1-integrin). , 1994, Blood.

[4]  L. McIntire,et al.  E-selectin supports neutrophil rolling in vitro under conditions of flow. , 1993, The Journal of clinical investigation.

[5]  R. Glanville,et al.  Neural crest cell interaction with type VI collagen is mediated by multiple cooperative binding sites within triple-helix and globular domains. , 1993, Experimental cell research.

[6]  R. Glanville,et al.  Collagen type VI in neural crest development: Distribution in situ and interaction with cells in vitro , 1993, Developmental dynamics : an official publication of the American Association of Anatomists.

[7]  R. Gordon,et al.  Co-localization of von Willebrand factor and type VI collagen in human vascular subendothelium. , 1993, The American journal of pathology.

[8]  J. Moake,et al.  Real-time analysis of shear-dependent thrombus formation and its blockade by inhibitors of von Willebrand factor binding to platelets. , 1993, Blood.

[9]  C. Kielty,et al.  Attachment of human vascular smooth muscles cells to intact microfibrillar assemblies of collagen VI and fibrillin. , 1992, Journal of cell science.

[10]  R. Glanville,et al.  Partial sequence of a candidate gene for the Marfan syndrome , 1991, Nature.

[11]  J. Rand,et al.  150-kD von Willebrand factor binding protein extracted from human vascular subendothelium is type VI collagen. , 1991, The Journal of clinical investigation.

[12]  R. Glanville,et al.  Extraction of extendable beaded structures and their identification as fibrillin-containing extracellular matrix microfibrils. , 1991, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[13]  R. Glanville,et al.  Orientation of type VI collagen monomers in molecular aggregates. , 1989, Biochemistry.

[14]  F. Bucciotti,et al.  Alpha 1 chain of chick type VI collagen. The complete cDNA sequence reveals a hybrid molecule made of one short collagen and three von Willebrand factor type A-like domains. , 1989 .

[15]  R. Timpl,et al.  Amino acid sequence of the triple-helical domain of human collagen type VI. , 1988, The Journal of biological chemistry.

[16]  L V McIntire,et al.  Shear stress induced stimulation of mammalian cell metabolism , 1988, Biotechnology and bioengineering.

[17]  L. McIntire,et al.  Effects of a novel antiplatelet agent in mural thrombogenesis on collagen-coated glass. , 1988, Blood.

[18]  K. Titani,et al.  Human von Willebrand factor: the molecular glue of platelet plugs. , 1988, Trends in biochemical sciences.

[19]  J. Koziol,et al.  Increased surface expression of the membrane glycoprotein IIb/IIIa complex induced by platelet activation. Relationship to the binding of fibrinogen and platelet aggregation. , 1987, Blood.

[20]  K. Titani,et al.  A heparin-binding domain of human von Willebrand factor. Characterization and localization to a tryptic fragment extending from amino acid residue Val-449 to Lys-728. , 1987, The Journal of biological chemistry.

[21]  K. Titani,et al.  CHARACTERIZATION OF THE VON WILLEBRAND FACTOR-BINDING DOMAIN OF PLATELET MEMBRANE GLYCOPROTEIN Ib , 1987, Thrombosis and Haemostasis.

[22]  J. Moake,et al.  Involvement of large plasma von Willebrand factor (vWF) multimers and unusually large vWF forms derived from endothelial cells in shear stress-induced platelet aggregation. , 1986, The Journal of clinical investigation.

[23]  E. Engvall,et al.  Fibrillin, a new 350-kD glycoprotein, is a component of extracellular microfibrils , 1986, The Journal of cell biology.

[24]  L. Holland,et al.  Isolation and characterization of a collagen binding domain in human von Willebrand factor. , 1986, Journal of Biological Chemistry.

[25]  K. Titani,et al.  The von Willebrand factor-binding domain of platelet membrane glycoprotein Ib. Characterization by monoclonal antibodies and partial amino acid sequence analysis of proteolytic fragments. , 1986, The Journal of biological chemistry.

[26]  H. Goldsmith,et al.  Rheological Aspects of Thrombosis and Haemostasis: Basic Principles and Applications , 1986, Thrombosis and Haemostasis.

[27]  R. D. Wade,et al.  Amino acid sequence of human von Willebrand factor. , 1986, Biochemistry.

[28]  J. Hubbell,et al.  Technique for visualization and analysis of mural thrombogenesis , 1986 .

[29]  E Ruoslahti,et al.  Platelet membrane glycoprotein IIb/IIIa: member of a family of Arg-Gly-Asp--specific adhesion receptors. , 1986, Science.

[30]  K. Titani,et al.  von Willebrand factor. A reduced and alkylated 52/48-kDa fragment beginning at amino acid residue 449 contains the domain interacting with platelet glycoprotein Ib. , 1986, The Journal of biological chemistry.

[31]  M. Gibson,et al.  CL glycoprotein is the tissue form of type VI collagen. , 1985, The Journal of biological chemistry.

[32]  R. Hynes,et al.  Interaction of fibronectin with its receptor on platelets , 1985, Cell.

[33]  R. Timpl,et al.  Immunochemistry, genuine size and tissue localization of collagen VI. , 1984, European journal of biochemistry.

[34]  B. Josefsson,et al.  Determination of amino acids with 9-fluorenylmethyl chloroformate and reversed-phase high-performance liquid chromatography , 1983 .

[35]  R. Montgomery,et al.  Platelets have more than one binding site for von Willebrand factor. , 1983, The Journal of clinical investigation.

[36]  R. Timpl,et al.  Electron-microscopical approach to a structural model of intima collagen. , 1983, The Biochemical journal.

[37]  M. Gibson,et al.  Elastin-associated microfibrils and microfibrillar proteins. , 1983, International review of connective tissue research.

[38]  J. Burch,et al.  Direct interaction of mepacrine with erythrocyte and platelet membrane phospholipid. , 1982, The Journal of biological chemistry.

[39]  L. Leung,et al.  Complex formation of platelet membrane glycoproteins IIb and IIIa with fibrinogen. , 1982, The Journal of clinical investigation.

[40]  C. Jenkin,et al.  Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose. , 1978, Immunochemistry.

[41]  M. Stemerman.  Vascular intimal components: precursors of thrombosis. , 1974, Progress in hemostasis and thrombosis.

[42]  H. R. Catchpole.  International Review of Connective Tissue Research , 1964 .