Synaptic vesicles recycling spontaneously and during activity belong to the same vesicle pool

Recently, it has been claimed that vesicles recycling spontaneously and during activity belong to different pools. Here we simultaneously measured, using spectrally separable styryl dyes, the release kinetics of vesicles recycled spontaneously or upon stimulation and the effects of the v-ATPase blocker folimycin on the frequency of miniature postsynaptic currents in rat hippocampal neurons. Our results provide evidence as to the identities of the vesicle pools recycling at rest and during stimulation.

[1]  R. Nicoll,et al.  Effects of reduced vesicular filling on synaptic transmission in rat hippocampal neurones , 2000, The Journal of physiology.

[2]  R. K. Simpson.  Nature Neuroscience , 2022 .

[3]  O. Prange,et al.  Correlation of Miniature Synaptic Activity and Evoked Release Probability in Cultures of Cortical Neurons , 1999, The Journal of Neuroscience.

[4]  R. Schneggenburger,et al.  Allosteric modulation of the presynaptic Ca2+ sensor for vesicle fusion , 2005, Nature.

[5]  E. Schuman,et al.  Miniature Neurotransmission Stabilizes Synaptic Function via Tonic Suppression of Local Dendritic Protein Synthesis , 2006, Cell.

[6]  D. Wilkin,et al.  Neuron , 2001, Brain Research.

[7]  Jean-Christophe Olivo-Marin,et al.  Extraction of spots in biological images using multiscale products , 2002, Pattern Recognit..

[8]  Thomas C. Südhof,et al.  Complexins Regulate a Late Step in Ca2+-Dependent Neurotransmitter Release , 2001, Cell.

[9]  Y. Sara,et al.  Phorbol Esters Target the Activity-Dependent Recycling Pool and Spare Spontaneous Vesicle Recycling , 2005, The Journal of Neuroscience.

[10]  T. Südhof,et al.  Autonomous Function of Synaptotagmin 1 in Triggering Synchronous Release Independent of Asynchronous Release , 2005, Neuron.

[11]  R. Rosenfeld.  Nature , 2009, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.

[12]  Marco Capogna,et al.  Miniature synaptic events maintain dendritic spines via AMPA receptor activation , 1999, Nature Neuroscience.

[13]  J. Rothman,et al.  A Clamping Mechanism Involved in SNARE-Dependent Exocytosis , 2006, Science.

[14]  W. Betz,et al.  Intraterminal Ca2+ and Spontaneous Transmitter Release at the Frog Neuromuscular Junction , 2001 .

[15]  J D Clements,et al.  Detection of spontaneous synaptic events with an optimally scaled template. , 1997, Biophysical journal.

[16]  B. Katz,et al.  Quantal components of the end‐plate potential , 1954, The Journal of physiology.

[17]  Xinran Liu,et al.  An Isolated Pool of Vesicles Recycles at Rest and Drives Spontaneous Neurotransmission , 2005, Neuron.