Cloning and Abiotic Stress Expression Pattern Analysis of Mulberry MYB Gene And Its Prokaryotic Expression In E. Coli

MYB transcription factors play a crucial regulatory role in plant growth, development, and response to abiotic stress. A c DNA sequence encoding for the MYB gene was cloned from Morus multicaulis variety YU-711. The gene has an open reading frame (ORF) of 882bp encoding 293 amino acid protein with a predicted molecular weight of 41.7 kDa and an isoelectric point (Pl) 7.61. The mulberry MYB gene is highly conserved. It contains SANT myb-like DNA-binding domain, SHAQKYF class, and belongs to the SNAT superfam - ily. The evolution analysis shows that the Morus multicaulis MYB of an unknown protein is closely related to the Morus notabilis . The qRT-PCR analysis revealed that the MYB gene is highly expressed in Mulberry and is regulated during abi - otic stress (salt, drought, and cold). There was an increase in the mRNA transcription level under all the stress treatment performed compared to the control. The western blot anal - ysis shows that the His-tag MYB protein was expressed in E . coli . Explaining the MYB protein and regulation that is pos - sible in Mulberry will provide the basis for the function and predict the contribution of MYB proteins to the biology of Mulberry plant during breeding for stress resistance variety

[1]  Long Li,et al.  Cloning and abiotic stress expression analysis of galactose-binding lectin (GBL) gene from mulberry and its prokaryotic expression in E. coli , 2020, The Journal of Horticultural Science and Biotechnology.

[2]  Narmada Thanki,et al.  CDD/SPARCLE: the conserved domain database in 2020 , 2019, Nucleic Acids Res..

[3]  Xiaoming Zhang,et al.  Mining MYB transcription factors from the genomes of orchids (Phalaenopsis and Dendrobium) and characterization of an orchid R2R3-MYB gene involved in water-soluble polysaccharide biosynthesis , 2019, Scientific Reports.

[4]  Y. Lyu,et al.  A MYB-Related Transcription Factor from Lilium lancifolium L. (LlMYB3) Is Involved in Anthocyanin Biosynthesis Pathway and Enhances Multiple Abiotic Stress Tolerance in Arabidopsis thaliana , 2019, International journal of molecular sciences.

[5]  Z. Xiang,et al.  An R1R2R3 MYB Transcription Factor, MnMYB3R1, Regulates the Polyphenol Oxidase Gene in Mulberry (Morus notabilis) , 2019, International journal of molecular sciences.

[6]  Jia Li,et al.  Overexpression of a MYB Family Gene, OsMYB6, Increases Drought and Salinity Stress Tolerance in Transgenic Rice , 2019, Front. Plant Sci..

[7]  R. Mishra,et al.  Selection of suitable reference genes for quantitative real-time PCR gene expression analysis in Mulberry (Morus alba L.) under different abiotic stresses , 2019, Molecular Biology Reports.

[8]  Long Li,et al.  Molecular cloning of potassium transporter gene, MaHAK5 of mulberry (Morus alba L.) and gene expression and biochemistry analysis under potassium stress , 2019 .

[9]  Yongqing Jiao,et al.  Overexpression of the Wild Soybean R2R3-MYB Transcription Factor GsMYB15 Enhances Resistance to Salt Stress and Helicoverpa Armigera in Transgenic Arabidopsis , 2018, International journal of molecular sciences.

[10]  Fengning Xiang,et al.  Drought Tolerance Conferred in Soybean (Glycine max. L) by GmMYB84, a Novel R2R3-MYB Transcription Factor , 2017, Plant & cell physiology.

[11]  E. Baldoni,et al.  Plant MYB Transcription Factors: Their Role in Drought Response Mechanisms , 2015, International journal of molecular sciences.

[12]  A. Osbourn,et al.  MYB Transcription Factors as Regulators of Phenylpropanoid Metabolism in Plants. , 2015, Molecular plant.

[13]  A. Xiong,et al.  Genome-wide analysis of the R2R3-MYB transcription factor genes in Chinese cabbage (Brassica rapa ssp. pekinensis) reveals their stress and hormone responsive patterns , 2015, BMC Genomics.

[14]  Guoxiang Liu,et al.  Characterization of a wheat R2R3-MYB transcription factor gene, TaMYB19, involved in enhanced abiotic stresses in Arabidopsis. , 2014, Plant & cell physiology.

[15]  D. Yamazaki,et al.  srGAP1 regulates lamellipodial dynamics and cell migratory behavior by modulating Rac1 activity , 2013, Molecular biology of the cell.

[16]  Yang-Dong Guo,et al.  The role of abscisic acid in fruit ripening and responses to abiotic stress. , 2013, Journal of experimental botany.

[17]  Guo-ping Zhang,et al.  Functional Characterization of cis-Elements Conferring Vascular Vein Expression of At4g34880 Amidase Family Protein Gene in Arabidopsis , 2013, PloS one.

[18]  N. Yadav,et al.  MYB transcription factor genes as regulators for plant responses: an overview , 2013, Physiology and Molecular Biology of Plants.

[19]  A. Katiyar,et al.  Genome-wide classification and expression analysis of MYB transcription factor families in rice and Arabidopsis , 2012, BMC Genomics.

[20]  E. Grotewold,et al.  Evolutionary and comparative analysis of MYB and bHLH plant transcription factors. , 2011, The Plant journal : for cell and molecular biology.

[21]  Yong Li,et al.  A single-repeat R3-MYB transcription factor MYBC1 negatively regulates freezing tolerance in Arabidopsis. , 2010, Biochemical and biophysical research communications.

[22]  Yi-Chieh Wang,et al.  A Novel MYBS3-Dependent Pathway Confers Cold Tolerance in Rice1[W][OA] , 2010, Plant Physiology.

[23]  Thomas D. Schmittgen,et al.  Analyzing real-time PCR data by the comparative CT method , 2008, Nature Protocols.

[24]  E. Grotewold,et al.  MYB transcription factors in Arabidopsis. , 2002, Trends in plant science.

[25]  H. Saedler,et al.  The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto‐oncogene products and with structural similarities to transcriptional activators. , 1987, The EMBO journal.

[26]  J. Bishop,et al.  Nucleotide sequence of the retroviral leukemia gene v-myb and its cellular progenitor c-myb: The architecture of a transduced oncogene , 1982, Cell.

[27]  王旭,et al.  Tricine—SDS—PAGE电泳分析小分子多肽 , 2003 .