Feasibility and Reproducibility of HIV-1 Genotype Resistance Test in Very-Low-Level Viremia

underlined the need to increase availability of assays to genotype samples with HIV-1 RNA levels of (cid:2) 400 copies/ml. In addition, the studies by Santoro et al. and Gonzalez-Serna et al. (2, 3) provide data to support reliability and usefulness of HIV-1 genotype resistance testing (GRT) in HIV-1 patients failing combined antiretroviral treatment (cART) with low-level viremia (LLV). These results are con-sidered predictive of future virologic outcomes (3). With the pres-ent letter, we would like to give our contribution, reporting on the results of sequencing samples not only with LLV but also with very low-level viremia (VLLV). the results obtained the 168 samples with HIV-1 RNA quantified below 1,000 copies/ml by the Versant HIV-1 RNA 1.0 assay Di-agnostics). HIV-1 RNA values of (cid:2) 50 copies/ml confirmed a Nuclisens HIV-1 2.0 assay