Effects of dairy manure and corn stover co-digestion on anaerobic microbes and corresponding digestion performance.

This study investigated the effects of corn stover as a supplemental feed on anaerobic digestion of dairy manure under different hydraulic retention times (HRT). The results elucidated that both HRT and corn stover supplement significantly influenced microbial community and corresponding anaerobic digestion performance. The highest biogas production of 497 mL per gram total solid loading per day was observed at a HRT of 40 days from digestion of manure supplemented with corn stover. Biogas production was closely correlated with the populations of Bacteroidetes, Clostridia and methanogens. Composition of the solid digestate (AD fiber) from the co-digestion of corn stover and dairy manure was similar to the digestion of dairy manure. However, the hydrolysis of AD fiber was significantly (P < 0.05) different among the different digestions. Both HRT and feed composition influenced the hydrolyzability of AD fiber via shifting the composition of microbial community.

[1]  B. White,et al.  Polysaccharide utilization by gut bacteria: potential for new insights from genomic analysis , 2008, Nature Reviews Microbiology.

[2]  Bahattin Topaloglu,et al.  Biogas production from co-digestion of a mixture of cheese whey and dairy manure , 2010 .

[3]  Nicholas E. Korres,et al.  Review of the integrated process for the production of grass biomethane. , 2009, Environmental science & technology.

[4]  S. Denman,et al.  Plant biomass degradation by gut microbiomes: more of the same or something new? , 2009, Current opinion in biotechnology.

[5]  M. Klocke,et al.  Archaea diversity within a commercial biogas plant utilizing herbal biomass determined by 16S rDNA and mcrA analysis , 2008, Journal of applied microbiology.

[6]  I. Good.  THE POPULATION FREQUENCIES OF SPECIES AND THE ESTIMATION OF POPULATION PARAMETERS , 1953 .

[7]  James R. Cole,et al.  The Ribosomal Database Project: improved alignments and new tools for rRNA analysis , 2008, Nucleic Acids Res..

[8]  Liza Gross.  Human Gut Hosts a Dynamically Evolving Microbial Ecosystem , 2007, PLoS biology.

[9]  H Harada,et al.  Recent advances in methane fermentation technology. , 2001, Current opinion in biotechnology.

[10]  Lars Ellegaard,et al.  Effects of mixing on methane production during thermophilic anaerobic digestion of manure: lab-scale and pilot-scale studies. , 2008, Bioresource technology.

[11]  Jorge Alberto Elizondo-Salazar,et al.  Estimación lineal de los requerimientos nutricionales del NRC para ganado de leche. , 2014 .

[12]  N. Ács,et al.  Biotechnological intensification of biogas production , 2007, Applied Microbiology and Biotechnology.

[13]  V. O’Flaherty,et al.  Methanogenic population structure in a variety of anaerobic bioreactors. , 2003, FEMS microbiology letters.

[14]  M. P. Bryant,et al.  Isolation and Characterization of Methanobacterium formicicum MF , 1987 .

[15]  Rob Knight,et al.  UCHIME improves sensitivity and speed of chimera detection , 2011, Bioinform..

[16]  Wei Liao,et al.  A sustainable pathway of cellulosic ethanol production integrating anaerobic digestion with biorefining , 2010, Biotechnology and bioengineering.

[17]  D. Boone,et al.  The Order Methanosarcinales , 2006 .

[18]  R. Huber,et al.  A cold-loving crenarchaeon is a substantial part of a novel microbial community in cold sulphidic marsh water. , 2006, FEMS microbiology ecology.

[19]  D. Boone,et al.  The Order Methanobacteriales , 2006 .

[20]  A. Morán,et al.  Anaerobic co-digestion of livestock wastes with vegetable processing wastes: a statistical analysis. , 2010, Bioresource technology.

[21]  Ann C Wilkie,et al.  Recovery of dairy manure nutrients by benthic freshwater algae. , 2002, Bioresource technology.

[22]  Wei Liao,et al.  FRESHWATER ALGAL CULTIVATION WITH ANIMAL WASTE FOR NUTRIENT REMOVAL AND BIOMASS PRODUCTION , 2012 .

[23]  Richard E. Speece,et al.  Anaerobic Biotechnology for Industrial Wastewaters , 1996 .

[24]  Charles E. Wyman,et al.  Cellulose and hemicellulose hydrolysis models for application to current and novel pretreatment processes , 2000 .

[25]  Wei Liao,et al.  Assessing solid digestate from anaerobic digestion as feedstock for ethanol production. , 2011, Bioresource technology.