Constitutive and inducible granulocyte-macrophage functions in mouse, rat, and human myeloid leukemia-derived continuous tissue culture lines.

Fourteen continuous tissue culture cell lines derived from mouse, rat, or human granulocyte-macrophage cancers were studied for expression of spontaneous and inducible markers of differentiated cells. Five cell lines (two mouse, two rat, and one human) synthesized myeloperoxidase spontaneously, and a fifth mouse line showed biochemically inducible enzyme. Twelve lines (6 mouse, 3 rat, and 3 human) produced lysozyme (muramidase), and all had detectable beta-glucuronidase. Superoxide generation was detected in one mouse, and three human cell lines following stimulation with phorbol myristate acetate. Maturation to differentiated polymorphonuclear leukocyte or macrophage morphology was induced in 3 cell lines (2 mouse and 1 human) following culture in diffusion chambers in total-body-irradiated rats. In vitro morphological differentiation was inducible in one (mouse) cell line exposed to casein, thioglycolate, or plasma from irradiated rats or mice. These findings indicate that mammalian cell lines derived from granulocyte-macrophage cancers stably express several combinations of differentiation markers. The patterns of expression of these markers did not always correlate with the morphological stage of differentiation.

[1]  S. Collins,et al.  Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[2]  H. Cohen,et al.  Superoxide generation by digitonin-stimulated guinea pig granulocytes. A basis for a continuous assay for monitoring superoxide production and for the study of the activation of the generating system. , 1978, The Journal of clinical investigation.

[3]  J. Greenberger,et al.  Increased myeloid-associated enzymes in Epstein-Barr virus nuclear antigen--positive human cell lines exposed to butyric acid in vitro. , 1978, Blood.

[4]  A. Epstein,et al.  Biology of the human malignant lymphomas. , 1978, Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer.

[5]  K. Nilsson,et al.  Cytochemical Profile of Human Haematopoietic Biopsy Cells and Derived Cell Lines , 1977, British journal of haematology.

[6]  S. Collins,et al.  Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture , 1977, Nature.

[7]  D. Rosenthal,et al.  Immunologic detection of intracellular and cell-surface lysozyme with human and experimental leukemic leukocytes. , 1977, Clinical Immunology and Immunopathology.

[8]  E. Kurrle,et al.  Evidence for differentiation of human leukemic blood cells in diffusion chamber culture. , 1977, Blood.

[9]  P. Ralph,et al.  Direct toxic effects of immunopotentiators on monocytic, myelomonocytic, and histiocytic or macrophage tumor cells in culture. , 1977, Cancer research.

[10]  J. Robinson,et al.  Host-determined differences in expression of surface marker characteristics on human and simian lymphoblastoid cell lines transformed by Epstein-Barr virus. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[11]  C. Lozzio,et al.  Properties of the K562 cell line derived from a patient with chronic myeloid leukemia. , 1977, International journal of cancer.

[12]  L. Sachs,et al.  Control of lysozyme induction in the differentiation of myeloid leukemic cells , 1976, Cell.

[13]  Peter Ralph,et al.  Properties of the K562 cell line, derived from a patient with chronic myeloid leukemia , 1976, International journal of cancer.

[14]  A. Zipursky,et al.  Leukocyte density and volume in normal subjects and in patients with acute lymphoblastic leukemia. , 1976, Blood.

[15]  M. Maeda,et al.  In vitro differentiation of Rauscher‐virus‐induced myeloid leukemia cells , 1976, International journal of cancer.

[16]  P. Ralph,et al.  Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines , 1976, The Journal of experimental medicine.

[17]  E. Klein,et al.  Comparative study of alkaline phosphatase activity in lymphocytes, mitogen-induced blasts, lymphoblastoid cell lines, acute myeloid leukemia, and chronic lymphatic leukemia cells. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[18]  D. Rosenthal,et al.  Continuous production of peroxidase, esterase, alkaline phosphatase and lysozyme by clones of promyelocytes , 1975, Nature.

[19]  P. Leder,et al.  Butyric acid, a potent inducer of erythroid differentiation in cultured erythroleukemic cells , 1975, Cell.

[20]  L. Fauerholdt,et al.  Cultivation of leukemic human bone marrow cells in diffusion chambers implanted into normal and irradiated mice. , 1975, Blood.

[21]  C. Lozzio,et al.  Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. , 1975, Blood.

[22]  A. Epstein,et al.  Biology of the human malignant lymphomas I. Establishment in continuous cell culture and heterotransplantation of diffuse histiocytic lymphomas , 1974, Cancer.

[23]  J. White,et al.  The influence of phorbol myristate acetate on oxygen consumption by polymorphonuclear leukocytes. , 1974, The Journal of laboratory and clinical medicine.

[24]  H. Metzger,et al.  THE INTERACTION OF IgE WITH RAT BASOPHILIC LEUKEMIA CELLS , 1974, The Journal of experimental medicine.

[25]  D. Housman,et al.  Effects of 5-bromo-2' -deoxyuridine on production of globin messenger RNA in dimethyl sulfoxide-stimulated Friend leukemia cells. , 1973, Proceedings of the National Academy of Sciences of the United States of America.

[26]  P. Leder,et al.  Globin messenger-RNA induction during erythroid differentiation of cultured leukemia cells. , 1972, Proceedings of the National Academy of Sciences of the United States of America.

[27]  E. Sadovsky,et al.  Evaluation of the leukocyte alkaline phosphatase activity. Comparison of the biochemical and cytochemical assay. , 1971, Clinica chimica acta; international journal of clinical chemistry.

[28]  W. Scher,et al.  Hemoglobin synthesis in murine virus-induced leukemic cells in vitro. I. Partial purification and identification of hemoglobins. , 1971, Blood.

[29]  W. Scher,et al.  Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide. , 1971, Proceedings of the National Academy of Sciences of the United States of America.

[30]  H. B. Benestad.  Formation of granulocytes and macrophages in diffusion chamber cultures of mouse blood leucocytes. , 2009, Scandinavian journal of haematology.

[31]  P. Duijn,et al.  CYTOPHOTOMETRIC DETERMINATION OF ALKALINE PHOSPHATASE ACTIVITY OF INDIVIDUAL NEUTROPHILIC LEUKOCYTES WITH A BIOCHEMICALLY CALIBRATED MODEL SYSTEM , 1968 .

[32]  L. Kaplow,et al.  Brief Report: Simplified Myeloperoxidase Stain Using Benzidine Dihydrochloride , 1965 .

[33]  L. Kaplow.  SIMPLIFIED MYELOPEROXIDASE STAIN USING BENZIDINE DIHYDROCHLORIDE. , 1965, Blood.

[34]  W. Moloney,et al.  ESTERASE ACTIVITY IN LEUKOCYTES DEMONSTRATED BY THE USE OF NAPHTHOL AS-D CHLOROACETATE SUBSTRATE , 1960, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[35]  V. Massey.  The microestimation of succinate and the extinction coefficient of cytochrome c. , 1959, Biochimica et biophysica acta.

[36]  M. L. Karnovsky,et al.  STUDIES ON THE INTERACTION BETWEEN PHAGOCYTES AND TUBERCLE BACILLI , 1956, The Journal of experimental medicine.

[37]  M. Menten,et al.  Distribution of Alkaline Phosphatase in Kidney Following the Use of Histochemical Azo Dye Test , 1944 .