论文信息 - Polymorphic microsatellite markers for studies of the conservation and reproductive genetics of imperilled sand tiger sharks (Carcharias taurus)

Polymorphic microsatellite markers for studies of the conservation and reproductive genetics of imperilled sand tiger sharks (Carcharias taurus)

We report on the isolation of eight microsatellites from the sand tiger shark, Carcharias taurus, using an enrichment protocol. All loci, with the exception of Cta45–183, were in Hardy–Weinberg equilibrium. Loci exhibited three to 15 alleles, and observed and expected heterozygosities of 0.095–1.000 and 0.284–0.924, respectively. An additional marker (Iox-12) developed from a shortfin mako library was variable in sand tigers. These markers will be used to examine population genetics and mating patterns of this imperilled species.

[1] M. Gillings,et al. Isolation and genetic diversity of endangered grey nurse shark (Carcharias taurus) populations , 2006, Biology Letters.

[2] A. Schrey,et al. Microsatellite markers for the shortfin mako and cross-species amplification in lamniformes , 2002, Conservation Genetics.

[3] T. C. Marshall,et al. Statistical confidence for likelihood‐based paternity inference in natural populations , 1998, Molecular ecology.

[4] François Rousset,et al. GENEPOP (version 1.2): population genetic software for exact tests and ecumenicism , 1995 .

[5] T. Glenn,et al. Isolating microsatellite DNA loci. , 2005, Methods in enzymology.

[6] L. Compagno,et al. Sharks of the world :an annotated and illustrated catalogue of shark species known to date , 1984 .