Measurement of alkaline phosphatase of intestinal origin in plasma by p-bromotetramisole inhibition.

L-p-bromotetramisole was used to inhibit non-intestinal alkaline phosphatase (of liver or bone origin) (EC 3.1.3.1; ALP) in plasma, and intestinal ALP was measured from the uninhibited activity. The method of determination is convenient and correlated well with measurement by immunocapture assay. If carried out in parallel with wheat-germ lectin precipitation of bone ALP, subtraction of intestinal ALP activity from that of non-bone ALP in the supernatant can be used to measure the ALP that originates from the liver in men and non-pregnant women.

[1]  T. Kuwana,et al.  Intestinal variant alkaline phosphatase in plasma in disease. , 1990, Clinical chemistry.

[2]  A. Y. Foo,et al.  Lectin affinity electrophoresis of alkaline phosphatase for the differentiation of bone and hepatobiliary disease , 1989, Electrophoresis.

[3]  M. D. de Broe,et al.  Multicenter evaluation of human placental alkaline phosphatase as a possible tumor-associated antigen in serum. , 1988, Clinical chemistry.

[4]  M. Hoylaerts,et al.  Improved agarose electrophoretic method for separating alkaline phosphatase isoenzymes in serum. , 1988, Clinical chemistry.

[5]  T. Mazda,et al.  Assay of alkaline phosphatase isoenzymes by a convenient precipitation and inhibition methodology. , 1988, Chemical & pharmaceutical bulletin.

[6]  A. Y. Foo,et al.  Two new methods for separating and quantifying bone and liver alkaline phosphatase isoenzymes in plasma. , 1984, Clinical chemistry.

[7]  Tietz Nw,et al.  IFCC methods for the measurement of catalytic concentration of enzymes Part 5. IFCC method for alkaline phosphatase (orthophosphoric-monoester phosphohydrolase, alkaline optimum, EC 3.1.3.1). , 1983, Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie.

[8]  R. O. Briere.  Alkaline phosphatase isoenzymes. , 1978, CRC critical reviews in clinical laboratory sciences.

[9]  M. D. de Broe,et al.  L-p-Bromotetramisole, a new reagent for use in measuring placental or intestinal isoenzymes of alkaline phosphatase in human serum. , 1977, Clinical chemistry.