A Novel Biomolecular Immobilization Matrix Based on Nanoporous ZnO／Chitosan Composite Film for Amperometric Hydrogen Peroxide Biosensor

A novel ZnO/Chitosan composite matrix was developed to fabricate the H2O2 biosensor.This material combined the advantages of inorganic species, ZnO, and organic polymer, chitosan.Horseradish peroxidase immobilized in the material maintained its activity well as the usage of glutaraldehyde was avoided. The activity of enzyme was 7.9 times greater than the cross-linked enzyme. The parameters affecting the fabrication and experimental conditions of biosensors were optimized. With the aid of hydroquinone mediator, the biosensor had a fast response of less than 10s. The linear range was 5.0×10-6 to 2. 0 × 10-3 mol/L with a sensitivity of 43.8μA L/mmol.This matrix can also be used to immobilize other biomolecule.