Triggered optical coherence tomography for capturing rapid periodic motion

Quantitative cross-sectional imaging of vocal folds during phonation is potentially useful for diagnosis and treatments of laryngeal disorders. Optical coherence tomography (OCT) is a powerful technique, but its relatively low frame rates makes it challenging to visualize rapidly vibrating tissues. Here, we demonstrate a novel method based on triggered laser scanning to capture 4-dimensional (4D) images of samples in motu at audio frequencies over 100 Hz. As proof-of-concept experiments, we applied this technique to imaging the oscillations of biopolymer gels on acoustic vibrators and aerodynamically driven vibrations of the vocal fold in an ex vivo calf larynx model. Our results suggest that triggered 4D OCT may be useful in understanding and assessing the function of vocal folds and developing novel treatments in research and clinical settings.

[1]  John A. Evans,et al.  Comprehensive volumetric optical microscopy in vivo , 2006, Nature Medicine.

[2]  R. Hillman,et al.  State of the art laryngeal imaging: research and clinical implications , 2010, Current opinion in otolaryngology & head and neck surgery.

[3]  Michael W. Jenkins,et al.  4D embryonic cardiography using gated optical coherence tomography. , 2006, Optics express.

[4]  Daryush D. Mehta,et al.  Assessment of Canine Vocal Fold Function after Injection of a New Biomaterial Designed to Treat Phonatory Mucosal Scarring , 2011, The Annals of otology, rhinology, and laryngology.

[5]  G. Christensen,et al.  A method for the reconstruction of four-dimensional synchronized CT scans acquired during free breathing. , 2003, Medical physics.

[6]  S. Yun,et al.  In vivo high-resolution video-rate spectral-domain optical coherence tomography of the human retina and optic nerve. , 2004, Optics express.

[7]  J. Kobler,et al.  Dynamic imaging of vocal fold oscillation with four‐dimensional optical coherence tomography , 2010, The Laryngoscope.

[8]  D. Bless,et al.  Videostroboscopic examination of the larynx , 1993 .

[9]  S H Yun,et al.  Motion artifacts in optical coherence tomography with frequency-domain ranging. , 2004, Optics express.

[10]  J. Kobler,et al.  Real‐time tracking of vocal fold injections with optical coherence tomography , 2009, The Laryngoscope.

[11]  S. Boppart,et al.  Acoustomotive optical coherence elastography for measuring material mechanical properties. , 2009, Optics letters.

[12]  A. Fercher,et al.  Performance of fourier domain vs. time domain optical coherence tomography. , 2003, Optics express.

[13]  Wolfgang Wieser,et al.  Multi-megahertz OCT: High quality 3D imaging at 20 million A-scans and 4.5 GVoxels per second. , 2010, Optics express.

[14]  J. Duker,et al.  Ultrahigh speed 1050nm swept source/Fourier domain OCT retinal and anterior segment imaging at 100,000 to 400,000 axial scans per second. , 2010, Optics express.

[15]  S. Yun,et al.  High-speed optical frequency-domain imaging. , 2003, Optics express.

[16]  Adrian Mariampillai,et al.  Doppler optical cardiogram gated 2D color flow imaging at 1000 fps and 4D in vivo visualization of embryonic heart at 45 fps on a swept source OCT system. , 2007, Optics express.

[17]  Changhuei Yang,et al.  Sensitivity advantage of swept source and Fourier domain optical coherence tomography. , 2003, Optics express.

[18]  D. Berry,et al.  High-speed digital imaging of the medial surface of the vocal folds. , 2001, The Journal of the Acoustical Society of America.

[19]  J. Izatt,et al.  High resolution imaging of in vivo cardiac dynamics using color Doppler optical coherence tomography. , 1997, Optics express.

[20]  G Gamsu,et al.  Gated MRI of cardiac and paracardiac masses: initial experience. , 1984, AJR. American journal of roentgenology.

[21]  A Quantitative Study of the Medial Surface Dynamics of an In Vivo Canine Vocal Fold during Phonation , 2005, The Laryngoscope.

[22]  Jack J. Jiang,et al.  A methodological study of hemilaryngeal phonation , 1993, The Laryngoscope.