Asian Journal of Microbiology and Biotechnology SYNTHESIS OF SILVER NANOPARTICLES (AgNPs) FROM BARK AND ROOT OF AFRICAN MAHOGANY (Khaya senegalensis) AND THE COMPARATIVE STUDIES OF..

Stem bark and root of Khaya senegalensis were extracted with ethyl acetate and n-Hexane and extracts screened for metabolites, antibacterial activity. There were also used to reduce AgNO3 to synthesize AgNPs and their antibacterial effects studied. A number of metabolite example flavonoids, steroids, tannins etc were discovered. The stem bark extract show higher activities on all the four bacterial isolates compared to the root extract: on K. pneumoniae (28.1±0.42), B. subtilis (20.1±1.21).while the root extract show lower activity on the test organisms at 26.3± 0.12 on K. pneumoniae and 22.1±0.20 on S. epidermidis. The activities of the nanoparticles are higher than those of the crude extracts and standard antibiotics used as control. The zones of inhibition around the bacterial colonies by the particles are far wider around each organism challenged. AgNPs bark extract on B. subtilis produced 31.2±0.11zone of inhibition of 24. 24.3±1.21 and 28.2±2.12 by Streptomycin and Tetracyclines. Strikingly, both the ethyl acetate and n-Hexane bark extracts of the plant demonstrated higher activities on the test isolates compared to the root extracts but the nanoparticles of the root had more activity compared to the stem bark. The activity of the metabolites in the root may have been enhanced when they were carried by the nanoparticles. The potency of Khaya senegalensis on microbial pathogens may be broadened when processed into its nanoparticles.

[1]  M. Baunthiyal,et al.  Characterization of silver nanoparticles synthesized using Urtica dioica Linn. leaves and their synergistic effects with antibiotics , 2016 .

[2]  H. Awobode,et al.  Antitrypanosomal activity of Khaya senegalensis and Anogeissus leiocarpus stem bark on Trypanosoma brucei brucei infected rats , 2015 .

[3]  M. Y. Gwarzo,et al.  Antibacterial activity of Khaya senegalensis stem bark extracts against some bacteria isolated from poultry droppings , 2015 .

[4]  H. Nagabhushana,et al.  Green synthesis of CuO nanoparticles using Gloriosa superba L. extract and their antibacterial activity , 2015 .

[5]  O. AnagaA..  Bioassay-guided Fractionation , Antihyperglycemic and Antioxidant Properties of the Methanol Leaf Extract of Helianthus annuus , 2015 .

[6]  D. Damisa,et al.  The Antibacterial Efficacy of Gold Nanoparticles Derived from Gomphrena celosioides and Prunus amygdalus (Almond) Leaves on Selected Bacterial Pathogens , 2014 .

[7]  O. A. Oyewole,et al.  Antibacterial Activities of Azadirachta Indica against Some Bacterial Pathogens , 2012 .

[8]  K. Shameli,et al.  Investigation of antibacterial properties silver nanoparticles prepared via green method , 2012, Chemistry Central Journal.

[9]  H. Domínguez,et al.  Protective effect against oxygen reactive species and skin fibroblast stimulation of Couroupita guianensis leaf extracts , 2012, Natural product research.

[10]  P. Mikaili,et al.  Etymological Review on Chemical and Pharmaceutical Substances of the Oriental Origin , 2012 .

[11]  A. Mann,et al.  Evaluation of the antimicrobial activities of two Ziziphus species (Ziziphus mauritiana L. and Ziziphus spinachristi L.) on some microbial pathogens , 2010 .

[12]  K. Govindaraju,et al.  Antimicrobial activity of silver nanoparticles Biogenic silver nanoparticles by Solanum torvum and their promising antimicrobial activity , 2010 .

[13]  P. Ajayan,et al.  Synthesis of gold and silver nanoparticles stabilized with glycosaminoglycans having distinctive biological activities. , 2009, Biomacromolecules.

[14]  V. Sharma,et al.  Silver nanoparticles: green synthesis and their antimicrobial activities. , 2009, Advances in colloid and interface science.

[15]  F. Adetuyi,et al.  Assessment of the antibacterial activity of Vernonia amygdalina and Occimum gratissimum leaves on selected food borne pathogens. , 2008 .

[16]  Kathryn M. Orzech,et al.  From Resilience to Resistance: Political Ecological Lessons from Antibiotic and Pesticide Resistance , 2008 .

[17]  A. Mann,et al.  Phytochemical and antibacterial screening of Anogeissus leiocarpus against some microorganisms associated with infectious wounds , 2008 .

[18]  J. van Staden,et al.  Assessing African medicinal plants for efficacy and safety: pharmacological screening and toxicology. , 2004, Journal of ethnopharmacology.

[19]  E. C. Fru,et al.  Anti-Salmonella activity of medicinal plants from Cameroon. , 2001, The Central African journal of medicine.

[20]  R. E. Omoregbe,et al.  Antimicrobial activity of some medicinal plants extracts on Escherichia coli, Salmonella paratyphi and Shigella dysenteriae. , 1996, African journal of medicine and medical sciences.

[21]  A. S. Zainal,et al.  Antimicrobial substance(s) in the leaf litter of the Xerophyte Prosopis juliflora , 1988 .