Disposable biosensor and biocatalysis of horseradish peroxidase based on sodium alginate film and room temperature ionic liquid.

Composite material based on room temperature ionic liquid (RTIL) N-butylpyridinium hexafluorophosphate (BPPF(6)), sodium alginate (SA), and graphite was used to construct a novel horseradish peroxidase (HRP) biosensor for the determination of H(2)O(2). The morphology of the as-prepared biosensor was characterized by scanning electron microscopy (SEM). Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to characterize the process of the performance of the biosensor. Parameters affecting the performance of the biosensor, including the concentrations of o-aminophenol (OAP) and HRP and the pH value of substrate solution, were optimized. Under the optimized experimental conditions, H(2)O(2) could be detected in a linear calibration range of 1.0 to 6.0 microM with a correlation coefficient of 0.9847 (n=7) and a detection limit of 0.5 microM at a signal/noise ratio of 3. The prepared biosensor not only had economic and disposable property but also showed good detection precision, bioactivity, storage stability, and reproducibility.

[1]  K. Soliman,et al.  Glioma cell antioxidant capacity relative to reactive oxygen species produced by dopamine , 2004, Journal of applied toxicology : JAT.

[2]  R. Hagiwara,et al.  Room temperature ionic liquids of alkylimidazolium cations and fluoroanions , 2000 .

[3]  J. Harrar.  Controlled-Potential Coulometric Determination of Hydrogen Peroxide. , 1963 .

[4]  P. Trulove,et al.  Direct X-Ray Diffraction Evidence for Imidazolium Intercalation into Graphite from an Ionic Liquid , 2003 .

[5]  S. Yao,et al.  Study of the immobilization of alcohol dehydrogenase on Au-colloid modified gold electrode by piezoelectric quartz crystal sensor, cyclic voltammetry, and electrochemical impedance techniques. , 2003, Journal of colloid and interface science.

[6]  Ping Sun,et al.  Ionic liquids in analytical chemistry. , 2010, Analytica chimica acta.

[7]  M. Goto,et al.  Solvent extraction and stripping of silver ions in room-temperature ionic liquids containing calixarenes. , 2004, Analytical chemistry.

[8]  P. Bonnesen,et al.  Extraction of cesium ions from aqueous solutions using calix[4]arene-bis(tert-octylbenzo-crown-6) in ionic liquids. , 2004, Analytical chemistry.

[9]  H. Ohno,et al.  Design of successive ion conduction paths in DNA films with ionic liquids , 2002 .

[10]  Yang Liu,et al.  An ionic liquid-type carbon paste electrode and its polyoxometalate-modified properties , 2005 .

[11]  Shusheng Zhang,et al.  Disposable biosensor based on enzyme immobilized on Au-chitosan-modified indium tin oxide electrode with flow injection amperometric analysis. , 2007, Analytical biochemistry.

[12]  F. Tajabadi,et al.  Simultaneous determination of dopamine, ascorbic acid, and uric acid using carbon ionic liquid electrode. , 2006, Analytical biochemistry.

[13]  Li Niu,et al.  Electrochemical functionalization of single-walled carbon nanotubes in large quantities at a room-temperature ionic liquid supported three-dimensional network electrode. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[14]  Y. N. He,et al.  Differential pulse voltammetric enzyme-linked immunoassay for the determination of Helicobacter pylori specific immunoglobulin G (IgG) antibody. , 1997, Talanta.

[15]  U. Karst,et al.  Simultaneous HPLC determination of peroxyacetic Acid and hydrogen peroxide. , 1997, Analytical chemistry.

[16]  Jinghong Li,et al.  Direct electron transfer of horseradish peroxidase and its biosensor based on chitosan and room temperature ionic liquid , 2006 .

[17]  Guobao Xu,et al.  Enhanced electrochemiluminescence sensor from tris(2,2'-bipyridyl)ruthenium(II) incorporated into MCM-41 and an ionic liquid-based carbon paste electrode. , 2007, The Analyst.

[18]  F. Tajabadi,et al.  High-performance carbon composite electrode based on an ionic liquid as a binder. , 2006, Analytical chemistry.

[19]  Zhao-Lun Fang,et al.  Direct extraction of double-stranded DNA into ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate and its quantification. , 2007, Analytical chemistry.

[20]  Jun Zhang,et al.  Molecular films of water-miscible ionic liquids formed on glassy carbon electrodes: characterization and electrochemical applications. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[21]  Robin D. Rogers,et al.  Ionic liquids : industrial applications for green chemistry , 2002 .

[22]  D. Haddleton,et al.  Copper(I) mediated living radical polymerisation in an ionic liquid , 2000 .

[23]  Y Komazaki,et al.  Automated measurement system for H2O2 in the atmosphere by diffusion scrubber sampling and HPLC analysis of Ti(IV)-PAR-H2O2 complex. , 2001, The Analyst.

[24]  H. Olivier-Bourbigou,et al.  Hydroformylation of 1-hexene with rhodium in non-aqueous ionic liquids : how to design the solvent and the ligand to the reaction , 2001 .

[25]  P. A. Tanner,et al.  Spectrophotometric determination of hydrogen peroxide in rainwater , 1998 .

[26]  R. Murray,et al.  An ionic liquid form of DNA: redox-active molten salts of nucleic acids. , 2001, Journal of the American Chemical Society.

[27]  Sandra Einloft,et al.  Synthesis and physical-chemical properties of ionic liquids based on 1-n-butyl-3-methylimidazolium cation , 1998 .

[28]  Zhihong Lin,et al.  A europium-ion-based luminescent sensing probe for hydrogen peroxide. , 2002, Angewandte Chemie.

[29]  Jinghong Li,et al.  Composite system based on chitosan and room-temperature ionic liquid: direct electrochemistry and electrocatalysis of hemoglobin. , 2006, Biomacromolecules.

[30]  Robin D. Rogers,et al.  Characterization and comparison of hydrophilic and hydrophobic room temperature ionic liquids incorporating the imidazolium cation , 2001 .

[31]  Masahiro Yoshizawa,et al.  Room temperature ionic liquids from 20 natural amino acids. , 2005, Journal of the American Chemical Society.

[32]  H.‐F. Wang,et al.  A Room Temperature Ionic Liquid (RTIL)‐Mediated, Non‐Hydrolytic Sol–Gel Methodology to Prepare Molecularly Imprinted, Silica‐Based Hybrid Monoliths for Chiral Separation , 2006 .

[33]  D. Marchington,et al.  H2O2 Determination by the I3- Method and by KMnO4 Titration , 1994 .

[34]  Wei Sun,et al.  Electrochemistry and electrocatalysis of hemoglobin in Nafion/nano-CaCO3 film on a new ionic liquid BPPF6 modified carbon paste electrode. , 2007, The journal of physical chemistry. B.