Production, Purification, and Application of Microbial Enzymes

The present human era aspires to change from chemical domination to biological domination. Enzymes are the biocatalysts required by all living organisms for syntheses as well as breakdown reactions. Enzymes are dynamic molecules and many harmful chemical reactions have been replaced by eco-friendly biological processes due to these biocatalysts. Enzyme technology is evolving rapidly. Enzymes, being highly specific in nature, have the capacity to revolutionize the whole industrial sector. Industrial enzyme production technology has attained a huge success in recent years due to major advancements in bioprocess technology. Enzymes with desired properties and improved functionality could be developed with the advent of genetic engineering as well as protein engineering. This chapter deals with industrial enzyme production, purification, formulation, commercial application, and provides a short account of the market position of enzymes globally. Novel enzymes as well as novel applications are continuing to emerge providing new opportunities for enzyme technology to flourish.

[1]  A. Chandel,et al.  Industrial enzymes in bioindustrial sector development: An Indian perspective , 2007 .

[2]  M. Reetz,et al.  Directed evolution and the creation of enantioselective biocatalysts , 2001, Applied Microbiology and Biotechnology.

[3]  A. Gaertner,et al.  FORMULATION OF DETERGENT ENZYMES , 1997 .

[4]  Túlio Marcos Santos,et al.  Up-To-Date Insight on Industrial Enzymes Applications and Global Market , 2012 .

[5]  A. Gusakov Cellulases and hemicellulases in the 21st century race for cellulosic ethanol , 2013 .

[6]  Christian Larroche,et al.  Current developments in solid-state fermentation , 2008 .

[7]  Amos Bairoch,et al.  The ENZYME database in 2000 , 2000, Nucleic Acids Res..

[8]  C. R. Soccol,et al.  Advances in microbial amylases. , 2000, Biotechnology and applied biochemistry.

[9]  A. Pandey Glucoamylase Research: An Overview , 1995 .

[10]  Ashok Pandey,et al.  MICROBIAL SYNTHESIS OF STARCH SACCHARIFYING ENZYME IN SOLID CULTURES , 1996 .

[11]  Carlos Ricardo Soccol,et al.  Recent advances in solid-state fermentation. , 2009 .

[12]  Paul A Dalby,et al.  Optimising enzyme function by directed evolution. , 2003, Current opinion in structural biology.

[13]  M E Groep,et al.  Performance modeling and simulation of biochemical process sequences with interacting unit operations. , 2000, Biotechnology and bioengineering.

[14]  O. Turunen,et al.  A de novo designed N-terminal disulphide bridge stabilizes the Trichoderma reesei endo-1,4-beta-xylanase II. , 2004, Journal of biotechnology.

[15]  Chiya Kuraishi,et al.  TRANSGLUTAMINASE: ITS UTILIZATION IN THE FOOD INDUSTRY , 2001 .

[16]  C. Soccol,et al.  Production, purification and properties of microbial phytases. , 2001, Bioresource technology.

[17]  Reeta Rani Singhania,et al.  An integrative process for bio-ethanol production employing SSF produced cellulase without extraction , 2015 .

[18]  L. Otten,et al.  Directed evolution: selecting today's biocatalysts. , 2005, Biomolecular engineering.

[19]  Ashok Pandey,et al.  Production, purification, characterization and over-expression of xylanases from actinomycetes. , 2013, Indian journal of experimental biology.

[20]  Parameswaran Binod,et al.  Industrial Enzymes - Present status and future perspectives for India , 2013 .

[21]  Ashok Pandey,et al.  The Industrial Production of Enzymes , 2010 .

[22]  C. Soccol,et al.  Solid state fermentation for the production of industrial enzymes , 1999 .

[23]  S. Chiang Strain improvement for fermentation and biocatalysis processes by genetic engineering technology , 2004, Journal of Industrial Microbiology and Biotechnology.

[24]  Z. Olempska-Beer,et al.  Food-processing enzymes from recombinant microorganisms--a review. , 2006, Regulatory toxicology and pharmacology : RTP.

[25]  Ashok Pandey,et al.  Solid-state fermentation , 1994 .