Characterization of Two Kinases Involved in Thiamine Pyrophosphate and Pyridoxal Phosphate Biosynthesis in Bacillus subtilis: 4-Amino-5-Hydroxymethyl-2-Methylpyrimidine Kinase and Pyridoxal Kinase

ABSTRACT Two Bacillus subtilis genes encoding two proteins (currently annotated ThiD and YjbV) were overexpressed and characterized. YjbV has 4-amino-5-hydroxymethyl-2-methylpyrimidine and 4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate kinase activity and should be reannotated ThiD, and B. subtilis ThiD has pyridoxine, pyridoxal, and pyridoxamine kinase activity and should be reannotated PdxK.

[1]  T. Begley,et al.  Mechanistic studies on thiamin phosphate synthase: evidence for a dissociative mechanism. , 2001, Biochemistry.

[2]  I. Mathews,et al.  Crystal structure of 4-methyl-5-beta-hydroxyethylthiazole kinase from Bacillus subtilis at 1.5 A resolution. , 2000, Biochemistry.

[3]  Sean V. Taylor,et al.  Thiamin biosynthesis in prokaryotes , 1999, Archives of Microbiology.

[4]  D. Smith,et al.  Cloning and characterization of the thiD/J gene of Escherichia coli encoding a thiamin-synthesizing bifunctional enzyme, hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase. , 1999, Microbiology.

[5]  M. Winkler,et al.  Overexpression, purification and characterization of two pyrimidine kinases involved in the biosynthesis of thiamin: 4-amino-5-hydroxymethyl-2-methylpyrimidine kinase and 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate kinase , 1998 .

[6]  D. Downs,et al.  Identification and characterization of an operon in Salmonella typhimurium involved in thiamine biosynthesis , 1997, Journal of bacteriology.

[7]  M. Winkler,et al.  Identification of the pdxK gene that encodes pyridoxine (vitamin B6) kinase in Escherichia coli K-12. , 1996, FEMS microbiology letters.

[8]  T. Mizote,et al.  The thiM locus and its relation to phosphorylation of hydroxyethylthiazole in Escherichia coli , 1989, Journal of bacteriology.

[9]  T. Mizote,et al.  Purification and properties of hydroxymethylpyrimidine kinase from Escherichia coli. , 1989, Biochimica et biophysica acta.

[10]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[11]  T. Begley,et al.  Mechanistic studies on thiamin phosphate synthase: evidence for a dissociative mechanism. , 2001, Biochemistry.

[12]  Sean V. Taylor,et al.  Thiamin biosynthesis in prokaryotes , 1999, Archives of Microbiology.

[13]  D. Smith,et al.  Cloning and characterization of the thiD/J gene of Escherichia coli encoding a thiamin-synthesizing bifunctional enzyme, hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase. , 1999, Microbiology.

[14]  M. Winkler,et al.  Overexpression, purification and characterization of two pyrimidine kinases involved in the biosynthesis of thiamin: 4-amino-5-hydroxymethyl-2-methylpyrimidine kinase and 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate kinase , 1998 .

[15]  D. Downs,et al.  Identification and characterization of an operon in Salmonella typhimurium involved in thiamine biosynthesis , 1997, Journal of bacteriology.

[16]  M. Winkler,et al.  Identification of the pdxK gene that encodes pyridoxine (vitamin B6) kinase in Escherichia coli K-12. , 1996, FEMS microbiology letters.

[17]  T. Mizote,et al.  The thiM locus and its relation to phosphorylation of hydroxyethylthiazole in Escherichia coli , 1989, Journal of bacteriology.

[18]  T. Mizote,et al.  Purification and properties of hydroxymethylpyrimidine kinase from Escherichia coli. , 1989, Biochimica et biophysica acta.

[19]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .