High field diffusion tensor imaging in small animals and excised tissue.

Molecular diffusion plays an important role in many biological phenomena. Magnetic Resonance (MR) imaging is inherently sensitive to diffusion and can be used to help understand diffusion processes. Diffusion MR imaging is most widely used for imaging the ischemic brain. Diffusion imaging and diffusion tensor imaging (DTI) have also found clinical application in areas such as tumor characterization throughout the body, imaging of demyelinating disorders, and fiber tract mapping. DTI is also now widely used in small animal imaging-both in vivo and in characterizing excised tissue. DTI studies in these settings can be accomplished with high resolution and can offer exquisite contrast, but the technical and practical challenges can sometimes be different than those seen on clinical MRI scanners. Here, a stepwise methodology is presented for using small-bore, high field strength scanners (>3 T) for DTI. This chapter is aimed at addressing readers with no prior knowledge of DTI and we present both a basic explanation of underlying principles and a practical approach to the experiment.

[1]  R. Kimmich.  NMR: Tomography, Diffusometry, Relaxometry , 2001 .

[2]  T. Michaelis,et al.  In vivo diffusion tensor mapping of the brain of squirrel monkey, rat, and mouse using single-shot STEAM MRI , 2004, Magnetic Resonance Materials in Physics, Biology and Medicine.

[3]  Tzu-Chao Chuang,et al.  PROPELLER EPI: An MRI technique suitable for diffusion tensor imaging at high field strength with reduced geometric distortions , 2005, Magnetic resonance in medicine.

[4]  D. Le Bihan,et al.  Diffusion tensor imaging: Concepts and applications , 2001, Journal of magnetic resonance imaging : JMRI.

[5]  P. Grenier,et al.  MR imaging of intravoxel incoherent motions: application to diffusion and perfusion in neurologic disorders. , 1986, Radiology.

[6]  Yoram Cohen,et al.  High b-value q-space diffusion MRI in myelin-deficient rat spinal cords. , 2006, Magnetic resonance imaging.

[7]  V. Wedeen,et al.  Reduction of eddy‐current‐induced distortion in diffusion MRI using a twice‐refocused spin echo , 2003, Magnetic resonance in medicine.

[8]  D. LeBihan,et al.  Molecular diffusion nuclear magnetic resonance imaging. , 1991 .

[9]  V. Gulani,et al.  Improved time efficiency and accuracy in diffusion tensor microimaging with multiple-echo acquisition. , 2005, Journal of magnetic resonance.

[10]  C F Hazlewood,et al.  Nuclear magnetic resonance measurement of skeletal muscle: anisotrophy of the diffusion coefficient of the intracellular water. , 1976, Biophysical journal.

[11]  N. Makris,et al.  High angular resolution diffusion imaging reveals intravoxel white matter fiber heterogeneity , 2002, Magnetic resonance in medicine.

[12]  Y. Ni,et al.  Diffusion weighted imaging in small rodents using clinical MRI scanners. , 2007, Methods.

[13]  J. E. Tanner,et al.  Spin diffusion measurements : spin echoes in the presence of a time-dependent field gradient , 1965 .

[14]  K. Hikishima,et al.  Volumetric q-space imaging by 3D diffusion-weighted MRI. , 2008, Magnetic resonance imaging.

[15]  M F Reiser,et al.  High‐resolution diffusion tensor imaging of human patellar cartilage: Feasibility and preliminary findings , 2005, Magnetic resonance in medicine.

[16]  Tong Zhu,et al.  A practical approach to in vivo high-resolution diffusion tensor imaging of rhesus monkeys on a 3-T human scanner. , 2009, Magnetic resonance imaging.

[17]  Y. Cohen,et al.  High b‐value q‐space analyzed diffusion‐weighted MRS and MRI in neuronal tissues – a technical review , 2002, NMR in biomedicine.

[18]  C. Beaulieu,et al.  Determinants of anisotropic water diffusion in nerves , 1994, Magnetic resonance in medicine.

[19]  M C Bushell,et al.  PRELIMINARY COMMUNICATION: The spatial mapping of translational diffusion coefficients by the NMR imaging technique , 1985 .

[20]  Hao Huang,et al.  Three-dimensional anatomical characterization of the developing mouse brain by diffusion tensor microimaging , 2003, NeuroImage.

[21]  John Crank,et al.  The Mathematics Of Diffusion , 1956 .

[22]  Vikas Gulani,et al.  Diffusion tensor magnetic resonance imaging. , 2006, Journal of neuro-ophthalmology : the official journal of the North American Neuro-Ophthalmology Society.

[23]  Denis Le Bihan,et al.  Imagerie de diffusion in-vivo par résonance magnétique nucléaire , 1985 .

[24]  Andre D. A. Souza,et al.  Indirect measurement of regional axon diameter in excised mouse spinal cord with q-space imaging: Simulation and experimental studies , 2008, NeuroImage.

[25]  E. Stejskal.  Use of Spin Echoes in a Pulsed Magnetic‐Field Gradient to Study Anisotropic, Restricted Diffusion and Flow , 1965 .

[26]  A. Anderson,et al.  Validation of diffusion tensor MRI‐based muscle fiber tracking , 2002, Magnetic resonance in medicine.

[27]  P. Basser,et al.  Estimation of the effective self-diffusion tensor from the NMR spin echo. , 1994, Journal of magnetic resonance. Series B.

[28]  Frauke Zipp,et al.  High-field diffusion tensor imaging of mouse brain in vivo using single-shot STEAM MRI , 2007, Journal of Neuroscience Methods.

[29]  H. C. Torrey.  Bloch Equations with Diffusion Terms , 1956 .

[30]  Jianhui Zhong,et al.  Diffusion measurements free of motion artifacts using intermolecular dipole‐dipole interactions , 2004, Magnetic resonance in medicine.

[31]  Yoram Cohen,et al.  Spatial and temporal damage evolution after hemi-crush injury in rat spinal cord obtained by high b-value q-space diffusion magnetic resonance imaging. , 2007, Journal of neurotrauma.

[32]  D G Gadian,et al.  Localized q‐space imaging of the mouse brain , 1997, Magnetic resonance in medicine.

[33]  Jens Frahm,et al.  Self-diffusion NMR imaging using stimulated echoes , 1985 .

[34]  J. Pope,et al.  Anisotropic water transport in the human eye lens studied by diffusion tensor NMR micro-imaging. , 2002, Experimental eye research.

[35]  L. Frank.  Characterization of anisotropy in high angular resolution diffusion‐weighted MRI , 2002, Magnetic resonance in medicine.

[36]  P. Basser,et al.  MR diffusion tensor spectroscopy and imaging. , 1994, Biophysical journal.