Ras-Raf interaction: two-hybrid analysis.

The identification of proteins mediating Ras effects, such as the serine/threonine kinases of the Raf family, has advanced our understanding of how signals are transmitted from the extracellular milieu to the nucleus. The modified two-hybrid system has proved to be a powerful tool for identifying specific protein interactions, such as those between Ras and Raf. We hope that the insight gained from the Ras screen, as well as insights from other two hybrid screens, will prove valuable in the application of this system to other enigmatic questions in biology.

[1]  M. Wigler,et al.  The S. cerevisiae CDC25 gene product regulates the RAS/adenylate cyclase pathway , 1987, Cell.

[2]  G. Fink,et al.  Laboratory course manual for methods in yeast genetics , 1986 .

[3]  R. Treisman,et al.  Characterization of SAP-1, a protein recruited by serum response factor to the c-fos serum response element , 1992, Cell.

[4]  S. Fields,et al.  Elimination of false positives that arise in using the two-hybrid system. , 1993, BioTechniques.

[5]  C J Marshall,et al.  Protein prenylation: a mediator of protein-protein interactions. , 1993, Science.

[6]  M. Boguski,et al.  Influence of guanine nucleotides on complex formation between Ras and CDC25 proteins , 1993, Molecular and cellular biology.

[7]  Frank McCormick,et al.  The GTPase superfamily: conserved structure and molecular mechanism , 1991, Nature.

[8]  R. Schiestl,et al.  Improved method for high efficiency transformation of intact yeast cells. , 1992, Nucleic acids research.

[9]  D. E. Griffiths,et al.  DMSO-enhanced whole cell yeast transformation. , 1991, Nucleic acids research.

[10]  D. Lowy,et al.  Mutational analysis of a ras catalytic domain , 1986, Molecular and cellular biology.

[11]  S. Fields,et al.  A novel genetic system to detect protein–protein interactions , 1989, Nature.

[12]  M. Wigler,et al.  Dominant yeast and mammalian RAS mutants that interfere with the CDC25-dependent activation of wild-type RAS in Saccharomyces cerevisiae , 1989, Molecular and cellular biology.

[13]  M Ptashne,et al.  DNA binding is not sufficient for nuclear localization of regulatory proteins in Saccharomyces cerevisiae , 1986, Molecular and cellular biology.

[14]  Jonathan A. Cooper,et al.  Mammalian Ras interacts directly with the serine/threonine kinase raf , 1993, Cell.

[15]  M. Wigler,et al.  In yeast, RAS proteins are controlling elements of adenylate cyclase , 1985, Cell.

[16]  R. Brent,et al.  Mxi1, a protein that specifically interacts with Max to bind Myc-Max recognition sites , 1993, Cell.

[17]  D. Hartley.  Cellular interactions in development : a practical approach , 1993 .