Further Studies on the Effect of Some Anti-Coagulants upon Sero-Diagnostic Tests for Syphilis

Ampleevidence isavailable thatbodyfluids other thanserumcontain antibodies (Coleman and Appleman, 1953;KanterandAppleton, 1940; Klauderand Kolmer,1921).Occasionally (Biro, 1947)thesefluids reveal antibodies more frequently andsoonerafter infection ofthebone marrowinsyphilis. Plasma alsohasanantibody content(Addis, 1912;Cowie,1909;Gurd, 1912), although some anti-coagulants interfere withthemeasurement ofsyphilitic reagin. However, upontheremoval ofthefibrinogen, someanticoagulants haveno appreciable effect uponthe serologic tests.Bloodtreated withpotassium oxalate inoptimal concentration can,therefore, beusedaswell asseruminthese tests. Theeffects ofthetreatment ofblood ontheserodiagnostic testsforsyphilis using thefollowing anti-coagulants werestudied: heparin, thymolsodium fluoride mixture, anisotonic potassium and ammoniumoxalate mixture, anda concentrated sodiumcitrate solution. Itwas observed that clotted bloodoften resists haemolysis oftheerythrocytes bybacterial lysis, enzymatic action, orphysical forces, considerably longer thanwilla sample of thesameblooddrawnatthesametimeandtreated withpotassium oxalate salts sufficient toprevent coagulation. Potassium oxalate, although not addedtotheblood-taking tubeswithsterile technique, willusually besterile uponbacterial culture, anditisdoubtful ifthesalt itself introduces microorganisms into theblood.

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