Highly Sensitive Electrochemical Non-Enzymatic Uric Acid Sensor Based on Cobalt Oxide Puffy Balls-like Nanostructure

Early-stage uric acid (UA) abnormality detection is crucial for a healthy human. With the evolution of nanoscience, metal oxide nanostructure-based sensors have become a potential candidate for health monitoring due to their low-cost, easy-to-handle, and portability. Herein, we demonstrate the synthesis of puffy balls-like cobalt oxide nanostructure using a hydrothermal method and utilize them to modify the working electrode for non-enzymatic electrochemical sensor fabrication. The non-enzymatic electrochemical sensor was utilized for UA determination using cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The puffy balls-shaped cobalt oxide nanostructure-modified glassy carbon (GC) electrode exhibited excellent electro-catalytic activity during UA detection. Interestingly, when we compared the sensitivity of non-enzymatic electrochemical UA sensors, the DPV technique resulted in high sensitivity (2158 µA/mM.cm2) compared to the CV technique (sensitivity = 307 µA/mM.cm2). The developed non-enzymatic electrochemical UA sensor showed good selectivity, stability, reproducibility, and applicability in the human serum. Moreover, this study indicates that the puffy balls-shaped cobalt oxide nanostructure can be utilized as electrode material for designing (bio)sensors to detect a specific analyte.

[1]  A. Nafady,et al.  Highly Heterogeneous Morphology of Cobalt Oxide Nanostructures for the Development of Sensitive and Selective Ascorbic Acid Non-Enzymatic Sensor , 2023, Biosensors.

[2]  Rafiq Ahmad,et al.  Electrochemical Ultrasensitive Sensing of Uric Acid on Non-Enzymatic Porous Cobalt Oxide Nanosheets-Based Sensor , 2022, Biosensors.

[3]  Y. Liu,et al.  Low-cost Voltammetric Sensors for Robust Determination of Toxic Cd(II) and Pb(II) in Environment and Food Based on Shuttle-like α-Fe2O3 Nanoparticles Decorated β-Bi2O3 Microspheres , 2022, Microchemical Journal.

[4]  J. Ahmed,et al.  Highly sensitive and selective non-enzymatic uric acid electrochemical sensor based on novel polypyrrole-carbon black-Co3O4 nanocomposite , 2022, Advanced Composites and Hybrid Materials.

[5]  A. Yu,et al.  A Double-Deck Structure of Reduced Graphene Oxide Modified Porous Ti3C2Tx Electrode towards Ultrasensitive and Simultaneous Detection of Dopamine and Uric Acid , 2021, Biosensors.

[6]  Jiadong Li,et al.  Highly Sensitive Uric Acid Detection Based on a Graphene Chemoresistor and Magnetic Beads , 2021, Biosensors.

[7]  Bruce Grieve,et al.  Recent Advances in Enzymatic and Non-Enzymatic Electrochemical Glucose Sensing , 2021, Sensors.

[8]  Rafiq Ahmad,et al.  Engineered CuO Nanofibers with Boosted Non-Enzymatic Glucose Sensing Performance , 2021, Journal of The Electrochemical Society.

[9]  A. Nafady,et al.  Silky Co3O4 nanostructures for the selective and sensitive enzyme free sensing of uric acid , 2021, RSC advances.

[10]  Rafiq Ahmad,et al.  High performance chemical sensor with field-effect transistors array for selective detection of multiple ions , 2020 .

[11]  F. Dai,et al.  Single-Atom Cobalt-Based Electrochemical Biomimetic Uric Acid Sensor with Wide Linear Range and Ultralow Detection Limit , 2020, Nano-Micro Letters.

[12]  Li Yang,et al.  A highly sensitive uric acid electrochemical biosensor based on a nano-cube cuprous oxide/ferrocene/uricase modified glassy carbon electrode , 2020, Scientific Reports.

[13]  Guangli Li,et al.  Morphology-dependent MnO2/nitrogen-doped graphene nanocomposites for simultaneous detection of trace dopamine and uric acid. , 2020, Materials science & engineering. C, Materials for biological applications.

[14]  Q. Hao,et al.  A novel electrochemical sensor for uric acid detection based on PCN/MWCNT , 2019, Ionics.

[15]  Muhammad Ishaq Abro,et al.  Facile Non‐enzymatic Lactic Acid Sensor Based on Cobalt Oxide Nanostructures , 2019, Electroanalysis.

[16]  Lintao Zeng,et al.  PtNi bimetallic nanoparticles loaded MoS2 nanosheets: Preparation and electrochemical sensing application for the detection of dopamine and uric acid. , 2019, Analytica chimica acta.

[17]  F. Jalali,et al.  Simultaneous determination of l‑DOPA, l‑tyrosine and uric acid by cysteic acid - modified glassy carbon electrode. , 2019, Materials science & engineering. C, Materials for biological applications.

[18]  K. Hussain,et al.  Comparison of enzymatic and non-enzymatic glucose sensors based on hierarchical Au-Ni alloy with conductive polymer. , 2019, Biosensors & bioelectronics.

[19]  Rafiq Ahmad,et al.  Deposition of nanomaterials: A crucial step in biosensor fabrication , 2018, Materials Today Communications.

[20]  S. Ponnaiah,et al.  New Electrochemical Sensor Based on a Silver-Doped Iron Oxide Nanocomposite Coupled with Polyaniline and Its Sensing Application for Picomolar-Level Detection of Uric Acid in Human Blood and Urine Samples. , 2018, The journal of physical chemistry. B.

[21]  J. Bonacin,et al.  Photochemical one-pot synthesis of reduced graphene oxide/Prussian blue nanocomposite for simultaneous electrochemical detection of ascorbic acid, dopamine, and uric acid , 2018 .

[22]  M. Krebsz,et al.  Recent Advances in Sensing Applications of Graphene Assemblies and Their Composites , 2017 .

[23]  Veerappan Mani,et al.  3D graphene oxide-cobalt oxide polyhedrons for highly sensitive non-enzymatic electrochemical determination of hydrogen peroxide , 2017 .

[24]  Rafiq Ahmad,et al.  Recent advances in nanowires-based field-effect transistors for biological sensor applications , 2017, Biosensors and Bioelectronics.

[25]  Tibor Pasinszki,et al.  Carbon Nanomaterial Based Biosensors for Non-Invasive Detection of Cancer and Disease Biomarkers for Clinical Diagnosis , 2017, Sensors.

[26]  Prashant K. Sharma,et al.  Probing the shape-specific electrochemical properties of cobalt oxide nanostructures for their application as selective and sensitive non-enzymatic glucose sensors , 2017 .

[27]  B. Yan,et al.  Dopamine and uric acid electrochemical sensor based on a glassy carbon electrode modified with cubic Pd and reduced graphene oxide nanocomposite. , 2017, Journal of colloid and interface science.

[28]  Rafiq Ahmad,et al.  Solution Process Synthesis of High Aspect Ratio ZnO Nanorods on Electrode Surface for Sensitive Electrochemical Detection of Uric Acid , 2017, Scientific Reports.

[29]  S. Ramesh,et al.  Enhanced electrochemical performance of cobalt oxide nanocube intercalated reduced graphene oxide for supercapacitor application , 2016 .

[30]  Ping Jiang,et al.  Nanoporous cobalt oxide nanowires for non-enzymatic electrochemical glucose detection , 2015 .

[31]  E. Holtzman,et al.  Uric acid levels within the normal range predict increased risk of hypertension: a cohort study. , 2015, Journal of the American Society of Hypertension : JASH.

[32]  Ping Yang,et al.  A facile electrochemical sensor based on reduced graphene oxide and Au nanoplates modified glassy carbon electrode for simultaneous detection of ascorbic acid, dopamine and uric acid , 2014 .

[33]  M. Arvand,et al.  Magnetic core-shell Fe₃O₄@SiO₂/MWCNT nanocomposite modified carbon paste electrode for amplified electrochemical sensing of uric acid. , 2014, Materials science & engineering. C, Materials for biological applications.

[34]  Z. Soltani,et al.  Potential Role of Uric Acid in Metabolic Syndrome, Hypertension, Kidney Injury, and Cardiovascular Diseases: Is It Time for Reappraisal? , 2013, Current Hypertension Reports.

[35]  Xiang Fang,et al.  Determination of serum uric acid using high-performance liquid chromatography (HPLC)/isotope dilution mass spectrometry (ID-MS) as a candidate reference method. , 2007, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[36]  M. Alderman,et al.  Uric acid: role in cardiovascular disease and effects of losartan , 2004, Current medical research and opinion.

[37]  Rafiq Ahmad,et al.  Hexagonal Cobalt Oxide Nanosheets Based Enzymeless Electrochemical Uric Acid Sensor with Improved Sensitivity , 2023, New Journal of Chemistry.

[38]  A. K. Hafiz,et al.  A Non-Enzymatic Electrochemical Sensor Composed of Nano-Berries Shaped Cobalt Oxide Nanostructures on Glassy Carbon Electrode for Uric Acid Detection , 2022, New Journal of Chemistry.

[39]  Y. Liu,et al.  Molecularly imprinted polypyrrole film-coated poly(3,4-ethylenedioxythiophene):polystyrene sulfonate-functionalized black phosphorene for the selective and robust detection of norfloxacin , 2022, Materials Today Chemistry.

[40]  K. Atacan,et al.  An electrochemical sensor based on Co3O4-ERGO nanocomposite modified screen-printed electrode for detection of uric acid in artificial saliva , 2021, Analytical Methods.

[41]  A. K. Hafiz,et al.  A highly sensitive uric acid biosensor based on vertically arranged ZnO nanorods on a ZnO nanoparticle-seeded electrode , 2021, New Journal of Chemistry.

[42]  M. Chan-Park,et al.  Electrochemical Detection of Uric Acid on Exfoliated Nanosheets of Graphitic-Like Carbon Nitride (g-C3N4) Based Sensor , 2019, Journal of The Electrochemical Society.

[43]  M. Lanaspa,et al.  Uric Acid as a Cause of the Metabolic Syndrome. , 2018, Contributions to nephrology.

[44]  Sen Liu,et al.  A combined self-assembly and calcination method for preparation of nanoparticles-assembled cobalt oxide nanosheets using graphene oxide as template and their application for non-enzymatic glucose biosensing. , 2017, Journal of colloid and interface science.

[45]  Rafiq Ahmad,et al.  Fabrication of highly sensitive uric acid biosensor based on directly grown ZnO nanosheets on electrode surface , 2015 .

[46]  P. Scharff,et al.  Multi-walled carbon nanotubes doped with boron as an electrode material for electrochemical studies on dopamine, uric acid, and ascorbic acid , 2015, Microchimica Acta.

[47]  Hiroshi Kataoka,et al.  Uric acid as a danger signal in gout and its comorbidities , 2013, Nature Reviews Rheumatology.