Electron Transfer Flavoprotein Subunit Beta Is a Candidate Endothelial Cell Autoantigen in Behçet’s Disease

Behçet’s disease (BD) is a chronic inflammatory disease with multisystem involvement, and it is listed as a rare disease in the United States but is common in the Middle East, China, and Japan. The aim of this study was to identify novel autoantigens in Chinese patients with BD. First, the candidate autoantigens were screened by Western blotting, and the sequences of putative antigens were identified by LC-MALDI-TOF/TOF mass spectrometry. Next, the screened protein was cloned, expressed and purified. Then, an optimized ELISA was developed, and the serological criteria were evaluated using a large number of confirmed patients. One antigen with a molecular weight of approximately 28 kDa was identified as electron transfer flavoprotein subunit beta (ETFB). Positive reactivity was detected in recombinant human ETFB sera from 38 of 92 BD patients (41 %) and 1 of 90 healthy controls (1 %).

[1]  Shutao Sun,et al.  Identification of heat shock protein 27 as a novel autoantigen of Behçet's disease. , 2015, Biochemical and biophysical research communications.

[2]  Weikang Yang,et al.  Identification of prohibitin as an antigen in Behcet's disease. , 2014, Biochemical and biophysical research communications.

[3]  H. Moutsopoulos.  Sjögren's syndrome: a forty-year scientific journey. , 2014, Journal of autoimmunity.

[4]  T. Huizinga,et al.  The pathogenic potential of autoreactive antibodies in rheumatoid arthritis , 2014, Seminars in Immunopathology.

[5]  R. Fang,et al.  The periplasmic PDZ domain-containing protein Prc modulates full virulence, envelops stress responses, and directly interacts with dipeptidyl peptidase of Xanthomonas oryzae pv. oryzae. , 2014, Molecular plant-microbe interactions : MPMI.

[6]  Haiming Jin,et al.  Generation and evaluation of antibodies against human MGF E-peptide by reverse phase protein microarray and reverse competitive ELISA. , 2013, Bioanalysis.

[7]  Shan Wang,et al.  Immunological screening and characterization of highly specific monoclonal antibodies against 20 kDa hGH. , 2012, Bioanalysis.

[8]  G. Geri,et al.  New insights into the pathogenesis of Behçet's disease. , 2012, Autoimmunity reviews.

[9]  J. H. Lee,et al.  Identification of HnRNP-A2/B1 as a target antigen of anti-endothelial cell IgA antibody in Behçet's disease. , 2012, The Journal of investigative dermatology.

[10]  K. Yudoh,et al.  Anti-endothelial cell antibodies (AECA) in patients with systemic vasculitis: our research using proteomics , 2011, Expert opinion on biological therapy.

[11]  S. Shin,et al.  Anti-Alpha-Enolase Antibody as a Serologic Marker and Its Correlation with Disease Severity in Intestinal Behçet’s Disease , 2011, Digestive Diseases and Sciences.

[12]  F. Frerman,et al.  The electron transfer flavoprotein: ubiquinone oxidoreductases. , 2010, Biochimica et biophysica acta.

[13]  J. Valente,et al.  Behçet's disease--a contemporary review. , 2009, Journal of autoimmunity.

[14]  T. Mak,et al.  Primer to the Immune Response , 2008 .

[15]  R. Froissart,et al.  Electron transfer flavoprotein deficiency: functional and molecular aspects. , 2006, Molecular genetics and metabolism.

[16]  A. Kavanaugh,et al.  4. Autoimmunity, vasculitis, and autoantibodies. , 2006, The Journal of allergy and clinical immunology.

[17]  A. Kavanaugh,et al.  Autoimmunity , vasculitis , and autoantibodies , 2006 .

[18]  S. Ghisla,et al.  Acyl-CoA dehydrogenases. A mechanistic overview. , 2004, European journal of biochemistry.

[19]  R. Vaughan,et al.  Behçet’s disease: from Hippocrates to the third millennium , 2003, The British journal of ophthalmology.

[20]  S. Shapiro,et al.  Anti-endothelial cell antibodies in Behçet's disease. , 2003, Clinical and experimental rheumatology.

[21]  I. Cohen,et al.  Identification of alpha‐tropomyosin as a target self‐antigen in Behçet's syndrome , 2002, European journal of immunology.

[22]  K. Bartlett.  Acyl‐CoA Dehydrogenases , 2002 .

[23]  O. Ozcebe,et al.  Behçet's disease. , 2019, The New England journal of medicine.

[24]  S. Ohno,et al.  Behçet's disease, the Silk Road and HLA-B51: historical and geographical perspectives. , 1999, Tissue antigens.

[25]  F. Frerman,et al.  Three-dimensional structure of human electron transfer flavoprotein to 2.1-A resolution. , 1996, Proceedings of the National Academy of Sciences of the United States of America.

[26]  G. Hughes,et al.  Antibodies to endothelial cells in patients with Behçet's disease. , 1993, Clinical immunology and immunopathology.

[27]  F. Frerman,et al.  Glutaric Acidemia Type II: Heterogeneity of Clinical and Biochemical Phenotypes , 1990, Pediatric Research.

[28]  A. Silman,et al.  [Criteria of diagnosis of Behcet's disease]. , 1975, La Tunisie medicale.

[29]  H. Beinert,et al.  The functional identity of the electron-transferring flavoproteins of the fatty acyl coenzyme A and sarcosine dehydrogenase systems. , 1962, The Journal of biological chemistry.

[30]  H. Beinert,et al.  On the mechanism of dehydrogenation of fatty acyl derivatives of coenzyme A. VI. Isolation and properties of stable enzyme-substrate complexes. , 1958, The Journal of biological chemistry.

[31]  F. L. Crane,et al.  On the mechanism of dehydrogenation of fatty acyl derivatives of coenzyme A. II. The electron-transferring flavoprotein. , 1956, The Journal of biological chemistry.