A novel nitrite biosensor based on the direct electrochemistry of hemoglobin immobilized on MXene-Ti3C2

Abstract Two-dimensional layered Ti3C2-based material (MXene-Ti3C2), which is a new graphene-like nanomaterials, has been synthesized and then used to immobilize hemoglobin (Hb) to fabricate a mediator-free biosensor. The morphology and structure of MXene-Ti3C2 were characterized by scanning electron microscopy and X-ray diffraction. Spectroscopic and electrochemical results revealed that MXene-Ti3C2 is an excellent immobilization matrix with biocompatibility for redox protein, affording good protein bioactivity and stability. Due to the large surface area and the high conductivity of MXene-Ti3C2, the direct electron transfer of Hb is facilitated and the prepared biosensors displayed good performance for the detection of nitrite with a wide linear range of 0.5–11800 μM, as well as an extremely low detection limit of 0.12 μM. This work suggests that MXene-Ti3C2 is a potential candidate for efficient enzyme immobilization and promising in environmental analysis.

[1]  Yang Fan,et al.  Direct electrochemistry of catalase at amine-functionalized graphene/gold nanoparticles composite film for hydrogen peroxide sensor , 2011 .

[2]  Allen J. Bard,et al.  Electrochemical Methods: Fundamentals and Applications , 1980 .

[3]  Yury Gogotsi,et al.  Two-dimensional transition metal carbides. , 2012, ACS nano.

[4]  Lo Gorton,et al.  Comment on "Direct electrochemistry and electrocatalysis of heme proteins entrapped in agarose hydrogel films in room-temperature ionic liquids". , 2005, Langmuir : the ACS journal of surfaces and colloids.

[5]  T. Kunitake,et al.  Protein Engineering Using Molecular Assembly: Functional Conversion of Cytochrome c via Noncovalent Interactions , 1997 .

[6]  Wei Sun,et al.  Direct electrochemistry of hemoglobin on graphene and titanium dioxide nanorods composite modified electrode and its electrocatalysis. , 2013, Biosensors & bioelectronics.

[7]  Hian Kee Lee,et al.  Direct electrochemistry and electrocatalysis of hemoglobin in nafion/carbon nanochip film on glassy carbon electrode. , 2009, The journal of physical chemistry. B.

[8]  Huixiang Li,et al.  Facile preparation of mesocellular graphene foam for direct glucose oxidase electrochemistry and sensitive glucose sensing , 2014 .

[9]  S. Elliott,et al.  Enzyme electrokinetics: using protein film voltammetry to investigate redox enzymes and their mechanisms. , 2003, Biochemistry.

[10]  Yuandong Xu,et al.  Hydrogen peroxide biosensor based on hemoglobin immobilized at graphene, flower-like zinc oxide, and gold nanoparticles nanocomposite modified glassy carbon electrode. , 2013, Colloids and surfaces. B, Biointerfaces.

[11]  Wensheng Yang,et al.  Nanosheet-based titania microspheres with hollow core-shell structure encapsulating horseradish peroxidase for a mediator-free biosensor. , 2011, Biomaterials.

[12]  Xiaomiao Feng,et al.  Direct electron transfer and electrocatalysis of hemoglobin immobilized on graphene–Pt nanocomposite , 2011 .

[13]  Wei Sun,et al.  Urchinlike MnO2 nanoparticles for the direct electrochemistry of hemoglobin with carbon ionic liquid electrode. , 2011, Biosensors & bioelectronics.

[14]  I. Naranjo-Rodríguez,et al.  Graphite grains studded with silver nanoparticles: description and application in promoting direct biocatalysis between heme protein and the resulting carbon paste electrode. , 2012, Colloids and surfaces. B, Biointerfaces.

[15]  Wei Sun,et al.  Application of graphene-copper sulfide nanocomposite modified electrode for electrochemistry and electrocatalysis of hemoglobin. , 2015, Biosensors & bioelectronics.

[16]  V. Presser,et al.  Two‐Dimensional Nanocrystals Produced by Exfoliation of Ti3AlC2 , 2011, Advanced materials.

[17]  Qingshan Lu,et al.  Immobilization and catalytic activity of horseradish peroxidase on molybdenum disulfide nanosheets modified electrode , 2013 .

[18]  Wei Sun,et al.  Application of N-doped graphene modified carbon ionic liquid electrode for direct electrochemistry of hemoglobin. , 2014, Materials science & engineering. C, Materials for biological applications.

[19]  Shengshui Hu,et al.  A reagentless nitric oxide biosensor based on the direct electrochemistry of hemoglobin adsorbed on the gold colloids modified carbon paste electrode , 2010 .

[20]  Jianbin Zheng,et al.  Direct electrochemistry and electrocatalysis of heme-proteins immobilized in porous carbon nanofiber/room-temperature ionic liquid composite film , 2010 .

[21]  Yang Fan,et al.  Direct electrochemistry and electrocatalysis of hemoglobin on chitosan-room temperature ionic liquid-TiO(2)-graphene nanocomposite film modified electrode. , 2011, Bioelectrochemistry.

[22]  E. Laviron.  General expression of the linear potential sweep voltammogram in the case of diffusionless electrochemical systems , 1979 .

[23]  Qing Zhang,et al.  Porous nanosheet-based ZnO microspheres for the construction of direct electrochemical biosensors. , 2008, Biosensors & bioelectronics.

[24]  Guang-Chao Zhao,et al.  Graphene-based modified electrode for the direct electron transfer of Cytochrome c and biosensing , 2010 .

[25]  Tinglin Huang,et al.  Direct electrochemistry and electrocatalysis of hemoglobin in composite film based on ionic liquid and NiO microspheres with different morphologies. , 2011, Biosensors & bioelectronics.