On target with a new mechanism for the regulation of protein phosphorylation.

There is overwhelming evidence that the reversible phosphorylation of proteins regulates most aspects of cell life. However, the broad specificities displayed by many protein phosphatases and kinases in vitro dictates that their activities be strictly regulated in vivo. Recent evidence indicates that a novel class of proteins, known as targetting subunits, specifies the location, catalytic and regulatory properties of protein phosphatases and kinases, and thereby plays a key role in ensuring the fidelity of protein phosphorylation.

[1]  T. Hunter,et al.  Cyclins and cancer , 1991, Cell.

[2]  M. Yanagida,et al.  Mitotic regulation of protein phosphatases by the fission yeast sds22 protein , 1993, Current Biology.

[3]  M. Bollen,et al.  The structure, role, and regulation of type 1 protein phosphatases. , 1992, Critical reviews in biochemistry and molecular biology.

[4]  T. Hunter,et al.  Human cyclins A and B1 are differentially located in the cell and undergo cell cycle-dependent nuclear transport , 1991, The Journal of cell biology.

[5]  M. Schilstra,et al.  A Lateral Cap model of microtubule dynamic instability , 1989, FEBS letters.

[6]  S. Jackson,et al.  The DNA-dependent protein kinase: Requirement for DNA ends and association with Ku antigen , 1993, Cell.

[7]  P. Cohen,et al.  Arachidonic acid inhibits myosin light chain phosphatase and sensitizes smooth muscle to calcium. , 1992, The Journal of biological chemistry.

[8]  P. Cohen,et al.  The glycogen-binding subunit of protein phosphatase-1G from rabbit skeletal muscle. Further characterisation of its structure and glycogen-binding properties. , 1989, European journal of biochemistry.

[9]  T. Hunter,et al.  Cyclin-dependent kinases: a new cell cycle motif? , 1991, Trends in cell biology.

[10]  P. Cohen,et al.  Identification of the major protein phosphatases in mammalian cardiac muscle which dephosphorylate phospholamban. , 1991, European journal of biochemistry.

[11]  A. Depaoli-Roach,et al.  Molecular cloning and expression of the regulatory (RG1) subunit of the glycogen-associated protein phosphatase. , 1991, The Journal of biological chemistry.

[12]  S. Leibler,et al.  Control of microtubule dynamics and length by cyclin A- and cyclin B- dependent kinases in Xenopus egg extracts , 1992, The Journal of cell biology.

[13]  D. Brautigan,et al.  Protein phosphatase type 1 in mammalian cell mitosis: chromosomal localization and involvement in mitotic exit , 1992, The Journal of cell biology.

[14]  P. Cohen,et al.  The protein phosphatases involved in cellular regulation. Purification and characterisation of the glycogen-bound form of protein phosphatase-1 from rabbit skeletal muscle. , 1985, European journal of biochemistry.

[15]  P. Dent,et al.  The molecular mechanism by which insulin stimulates glycogen synthesis in mammalian skeletal muscle , 1990, Nature.

[16]  P. Cohen,et al.  Regulation of protein phosphatase-1G from rabbit skeletal muscle. 1. Phosphorylation by cAMP-dependent protein kinase at site 2 releases catalytic subunit from the glycogen-bound holoenzyme. , 1989, European journal of biochemistry.

[17]  P. Cohen,et al.  The control of protein phosphatase-1 by targetting subunits. The major myosin phosphatase in avian smooth muscle is a novel form of protein phosphatase-1. , 1992, European journal of biochemistry.

[18]  P. Cohen,et al.  Regulation of protein phosphatase-1G from rabbit skeletal muscle. 2. Catalytic subunit translocation is a mechanism for reversible inhibition of activity toward glycogen-bound substrates. , 1989, European journal of biochemistry.

[19]  M. Yanagida,et al.  S. pombe gene sds22 + essential for a midmitotic transition encodes a leucine-rich repeat protein that positively modulates protein phosphatase-1 , 1991, Cell.

[20]  P. Cohen.  The structure and regulation of protein phosphatases. , 1989, Annual review of biochemistry.

[21]  G. Draetta,et al.  Cell cycle control in eukaryotes: molecular mechanisms of cdc2 activation. , 1990, Trends in biochemical sciences.

[22]  E. Karsenti,et al.  Phosphorylation and activation of human cdc25‐C by cdc2‐‐cyclin B and its involvement in the self‐amplification of MPF at mitosis. , 1993, The EMBO journal.

[23]  S. Lohmann,et al.  Identification of a high affinity binding protein for the regulatory subunit RII beta of cAMP‐dependent protein kinase in Golgi enriched membranes of human lymphoblasts. , 1992, The EMBO journal.

[24]  M. Bollen,et al.  Purification and characterization of the glycogen-bound protein phosphatase from rat liver. , 1991, The Journal of biological chemistry.

[25]  J. Nevins,et al.  A cyclin A-protein kinase complex possesses sequence-specific DNA binding activity: p33cdk2 is a component of the E2F-cyclin A complex , 1992, Cell.

[26]  R. Cone,et al.  Localization of the cAMP-dependent protein kinase to the postsynaptic densities by A-kinase anchoring proteins. Characterization of AKAP 79. , 1992, The Journal of biological chemistry.

[27]  P. Cohen,et al.  Targetting of protein phosphatase 1 to the sarcoplasmic reticulum of rabbit skeletal muscle by a protein that is very similar or identical to the G subunit that directs the enzyme to glycogen. , 1990, European journal of biochemistry.

[28]  A. Prescott,et al.  PPX, a novel protein serine/threonine phosphatase localized to centrosomes. , 1993, The EMBO journal.

[29]  Hui Zhang,et al.  D type cyclins associate with multiple protein kinases and the DNA replication and repair factor PCNA , 1992, Cell.

[30]  D. Mochly‐Rosen,et al.  Intracellular receptors for activated protein kinase C. Identification of a binding site for the enzyme. , 1991, The Journal of biological chemistry.

[31]  P. Cohen,et al.  Further studies on the structure of the glycogen-bound form of protein phosphatase-1 from rabbit skeletal muscle. , 1987, European journal of biochemistry.

[32]  D. Bregman,et al.  Molecular characterization of bovine brain P75, a high affinity binding protein for the regulatory subunit of cAMP-dependent protein kinase II beta. , 1991, The Journal of biological chemistry.

[33]  P. Cohen,et al.  A myofibrillar protein phosphatase from rabbit skeletal muscle contains the beta isoform of protein phosphatase-1 complexed to a regulatory subunit which greatly enhances the dephosphorylation of myosin. , 1992, European journal of biochemistry.

[34]  B. Buendia,et al.  Regulation of the microtubule nucleating activity of centrosomes in Xenopus egg extracts: role of cyclin A-associated protein kinase , 1992, The Journal of cell biology.

[35]  R. Fletterick,et al.  The family of glycogen phosphorylases: structure and function. , 1989, Critical reviews in biochemistry and molecular biology.

[36]  P. Cohen,et al.  Identification of three in vivo phosphorylation sites on the glycogen‐binding subunit of protein phosphatase 1 from rabbit skeletal muscle, and their response to adrenaline , 1990, FEBS letters.

[37]  C. Smythe,et al.  Coupling of mitosis to the completion of S phase in Xenopus occurs via modulation of the tyrosine kinase that phosphorylates p34 cdc2 , 1992, Cell.

[38]  K. Tatchell,et al.  GAC1 may encode a regulatory subunit for protein phosphatase type 1 in Saccharomyces cerevisiae. , 1992, The EMBO journal.