Recent advances in immobilization methods of antibodies on solid supports.

Antibody immobilization on a solid support is an essential process for the development of most immune-based assay systems. The choice of the immobilization method greatly affects antibody-antigen interactions on the assay surface. For the past several years, numerous strategies have been reported to control antibody immobilization, mainly by directing the orientation, stability, and density of bound antibodies on different assay platforms. Here we discuss recent developments in antibody immobilization methods with a particular focus on the strengths and limitations of reported approaches, and thereby provide a useful guideline for the selection of suitable antibody coupling procedures.

[1]  Michael J Taussig,et al.  Improved affinity coupling for antibody microarrays: Engineering of double‐(His)6‐tagged single framework recombinant antibody fragments , 2006, Proteomics.

[2]  Soo-Ik Chang,et al.  Profiling of differential protein expression in angiogenin‐induced HUVECs using antibody‐arrayed ProteoChip , 2006, Proteomics.

[3]  D. Wong,et al.  Self-assembled layer of thiolated protein G as an immunosensor scaffold. , 2007, Analytical chemistry.

[4]  Eun Kyu Lee,et al.  Improving immunobinding using oriented immobilization of an oxidized antibody. , 2007, Journal of chromatography. A.

[5]  Agustín Costa-García,et al.  Oriented immobilisation of anti-pneumolysin Fab through a histidine tag for electrochemical immunosensors. , 2007, Biosensors & bioelectronics.

[6]  J. Lee,et al.  Oriented Immobilization of Antibodies with GST-Fused Multiple Fc-Specific B-Domains on a Gold Surface , 2007 .

[7]  Bong Hyun Chung,et al.  Self-directed and self-oriented immobilization of antibody by protein G-DNA conjugate. , 2007, Analytical chemistry.

[8]  Carla B. Swearingen,et al.  Direct immobilization of Fab' in nanocapillaries for manipulating mass-limited samples. , 2007, Journal of the American Chemical Society.

[9]  Wilfred Chen,et al.  Fabrication of antibody arrays using thermally responsive elastin fusion proteins. , 2006, Journal of the American Chemical Society.

[10]  Jin Kyeong Kim,et al.  Direct immobilization of protein g variants with various numbers of cysteine residues on a gold surface. , 2007, Analytical chemistry.

[11]  A. Gorse,et al.  High-throughput optimization of surfaces for antibody immobilization using metal complexes. , 2007, Analytical biochemistry.

[12]  H. Hug,et al.  Advances in recombinant antibody microarrays. , 2004, Clinica chimica acta; international journal of clinical chemistry.

[13]  Jiri Homola,et al.  DNA directed protein immobilization on mixed ssDNA/oligo(ethylene glycol) self-assembled monolayers for sensitive biosensors. , 2004, Analytical chemistry.

[14]  Yoonsuk Lee,et al.  ProteoChip: A highly sensitive protein microarray prepared by a novel method of protein immobilization for application of protein‐protein interaction studies , 2003, Proteomics.

[15]  Chad A Mirkin,et al.  Functional Antibody Arrays through Metal Ion‐Affinity Templates , 2006, Chembiochem : a European journal of chemical biology.

[16]  O. Hofstetter,et al.  A comparative evaluation of random and site-specific immobilization techniques for the preparation of antibody-based chiral stationary phases. , 2006, Journal of separation science.

[17]  M. T. Neves-Petersen,et al.  Light‐induced immobilisation of biomolecules as an attractive alternative to microdroplet dispensing‐based arraying technologies , 2007, Proteomics.

[18]  Robyn L Stanfield,et al.  Contrasting IgG structures reveal extreme asymmetry and flexibility. , 2002, Journal of molecular biology.

[19]  Inger Vikholm-Lundin,et al.  Site-directed immobilisation of antibody fragments for detection of C-reactive protein. , 2006, Biosensors & bioelectronics.

[20]  Lu Zhang,et al.  Construction of an antibody microarray based on agarose‐coated slides , 2007, Electrophoresis.

[21]  Milan Mrksich,et al.  Self-assembled monolayers for MALDI-TOF mass spectrometry for immunoassays of human protein antigens. , 2007, Analytical chemistry.

[22]  Shaoyi Jiang,et al.  DNA-directed protein immobilization for simultaneous detection of multiple analytes by surface plasmon resonance biosensor. , 2006, Analytical chemistry.

[23]  T. Webster,et al.  Comparison of antibody functionality using different immobilization methods , 2003, Biotechnology and bioengineering.

[24]  Mu-San Chen,et al.  Fabrication and characterization of 3D hydrogel microarrays to measure antigenicity and antibody functionality for biosensor applications. , 2004, Biosensors & bioelectronics.

[25]  Esteve Fàbregas,et al.  New antibodies immobilization system into a graphite-polysulfone membrane for amperometric immunosensors. , 2007, Biosensors & bioelectronics.

[26]  Gabriel A Kwong,et al.  DNA-encoded antibody libraries: a unified platform for multiplexed cell sorting and detection of genes and proteins. , 2007, Journal of the American Chemical Society.

[27]  S. Cosnier,et al.  Electrogenerated indium tin oxide-coated glass surface with photosensitive interfaces: surface analysis. , 2007, Biosensors & bioelectronics.

[28]  Jizhong Zhou,et al.  Protein microarrays on hybrid polymeric thin films prepared by self‐assembly of polyelectrolytes for multiple‐protein immunoassays , 2006, Proteomics.

[29]  G. Auner,et al.  Investigation of spacer length effect on immobilized Escherichia coli pili‐antibody molecular recognition by AFM , 2007, Biotechnology and bioengineering.

[30]  Ursula Bilitewski,et al.  Protein-sensing assay formats and devices. , 2006, Analytica chimica acta.

[31]  E. Voss,et al.  The immunochemistry of sandwich ELISAs--VI. Greater than 90% of monoclonal and 75% of polyclonal anti-fluorescyl capture antibodies (CAbs) are denatured by passive adsorption. , 1993, Molecular immunology.

[32]  L Torrance,et al.  Oriented immobilisation of engineered single-chain antibodies to develop biosensors for virus detection. , 2006, Journal of virological methods.

[33]  Il-Hoon Cho,et al.  Site-directed biotinylation of antibodies for controlled immobilization on solid surfaces. , 2007, Analytical biochemistry.

[34]  M. T. Neves-Petersen,et al.  Photonic activation of disulfide bridges achieves oriented protein immobilization on biosensor surfaces , 2006, Protein science : a publication of the Protein Society.