BACKGROUND: Recently, the role of neutrophil extracellular traps (NETs) in oncopathology, including colorectal cancer (CRC), has become a subject of discussion. Literature review showed that in CRC, there are separate experimental and clinical studies devoted to assess the ability of neutrophils to form NET. A methodological approach, common to these studies, was that the ability of blood neutrophils in patients with CRC to form NET was evaluated in vitro.
AIM: We undertook a study of the NETs presence in the tumor tissues, on the periphery of the tumor, and in healthy tissues with CRC at different stages and different types of therapy.
METHODS: The study was carried out in the framework of the scientific and technical program “Personalized approach to the management of number of significant diseases” developed by the Non-commercial Joint-Stock Company “Karaganda Medical University,” funded by the Ministry of Education and Science of the Republic of Kazakhstan.
RESULTS: Patients with CRC II and III stages are more likely to have NET in the center, on the periphery of the tumor and in healthy tissues adjacent to the tumor than in patients with CRC of Stage I. It was also found that radiation therapy as a pre-operative preparation contributed to an increase in the number of NET in the center of the tumor in patients with Stage I rectal cancer.
CONCLUSION: Thus, our data revealed an increase in NET in the center and periphery of CRC, and an increase in their number after pre-operative radiation therapy was shown. The next stage of our study will be devoted to study the structural features of NET and their interaction with other effector cells and tumor cells.
[1]
H. Thorlacius,et al.
Neutrophil extracellular traps promote peritoneal metastasis of colon cancer cells
,
2019,
Oncotarget.
[2]
A. Jemal,et al.
Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries
,
2018,
CA: a cancer journal for clinicians.
[3]
V. Brinkmann.
Neutrophil Extracellular Traps in the Second Decade
,
2018,
Journal of Innate Immunity.
[4]
D. Thickett,et al.
Neutrophil Extracellular Trap Production in Patients with Colorectal Cancer In Vitro
,
2017,
International journal of inflammation.
[5]
A. Giatromanolaki,et al.
Gradient Infiltration of Neutrophil Extracellular Traps in Colon Cancer and Evidence for Their Involvement in Tumour Growth
,
2016,
PloS one.
[6]
P. Kubes,et al.
NETosis: how vital is it?
,
2013,
Blood.
[7]
B. Turner.
International Union Against Cancer (UICC)
,
2019,
B-Model Gromov-Witten Theory.
[8]
G. Braemer.
International statistical classification of diseases and related health problems. Tenth revision.
,
1988,
World health statistics quarterly. Rapport trimestriel de statistiques sanitaires mondiales.