Site- and subunit-specific incorporation of unnatural amino acids into HIV-1 reverse transcriptase.
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G. Klarmann | S. L. Le Grice | S. Hecht | B. M. Eisenhauer | D. K. Chatterjee | K. Sitaraman | Yi Zhang
[1] Dominic Esposito,et al. A novel cell-free protein synthesis system. , 2004, Journal of biotechnology.
[2] Samuel H. Wilson,et al. High-level expression and purification of untagged and histidine-tagged HIV-1 reverse transcriptase. , 2004, Protein Expression and Purification.
[3] P. Schultz,et al. Expanding the Genetic Code , 2003, Science.
[4] Peter G Schultz,et al. An Expanded Eukaryotic Genetic Code , 2003, Science.
[5] Brian A. Smith,et al. A new strategy for the site-specific modification of proteins in vivo. , 2003, Biochemistry.
[6] H. Lester,et al. Caging proteins through unnatural amino acid mutagenesis. , 2003, Methods in enzymology.
[7] P. Schultz,et al. In Vivo Photocrosslinking with Unnatural Amino Acid Mutagenesis , 2002, Chembiochem : a European journal of chemical biology.
[8] S. Hecht,et al. Fluorescence resonance energy transfer between unnatural amino acids in a structurally modified dihydrofolate reductase. , 2002, Journal of the American Chemical Society.
[9] Andrew B. Martin,et al. Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli , 2002, Proceedings of the National Academy of Sciences of the United States of America.
[10] P. Schultz,et al. Adding L-3-(2-Naphthyl)alanine to the genetic code of E. coli. , 2002, Journal of the American Chemical Society.
[11] K. Terpe. Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems , 2002, Applied Microbiology and Biotechnology.
[12] Liang Xie,et al. Import of amber and ochre suppressor tRNAs into mammalian cells: A general approach to site-specific insertion of amino acid analogues into proteins , 2001, Proceedings of the National Academy of Sciences of the United States of America.
[13] P. Schultz,et al. A general approach for the generation of orthogonal tRNAs. , 2001, Chemistry & biology.
[14] Takuya Ueda,et al. Cell-free translation reconstituted with purified components , 2001, Nature Biotechnology.
[15] P G Schultz,et al. Expanding the Genetic Code of Escherichia coli , 2001, Science.
[16] A. D. Clark,et al. Crystal structure of HIV‐1 reverse transcriptase in complex with a polypurine tract RNA:DNA , 2001, The EMBO journal.
[17] S. L. Le Grice,et al. Evaluation of retroviral ribonuclease H activity. , 2001, Methods in molecular biology.
[18] D. A. Dougherty,et al. Unnatural amino acids as probes of protein structure and function. , 2000, Current opinion in chemical biology.
[19] G. F. Short,et al. Probing the S1/S1' substrate binding pocket geometry of HIV-1 protease with modified aspartic acid analogues. , 2000, Biochemistry.
[20] T. Schmidt,et al. Use of the Strep-Tag and streptavidin for detection and purification of recombinant proteins. , 2000, Methods in enzymology.
[21] G. F. Short,et al. Effects of release factor 1 on in vitro protein translation and the elaboration of proteins containing unnatural amino acids. , 1999, Biochemistry.
[22] P. Schultz,et al. Progress toward the evolution of an organism with an expanded genetic code. , 1999, Proceedings of the National Academy of Sciences of the United States of America.
[23] G L Verdine,et al. Structure of a covalently trapped catalytic complex of HIV-1 reverse transcriptase: implications for drug resistance. , 1998, Science.
[24] V. Pandey,et al. Loss of polymerase activity due to Tyr to Phe substitution in the YMDD motif of human immunodeficiency virus type-1 reverse transcriptase is compensated by Met to Val substitution within the same motif. , 1998, Biochemistry.
[25] G. Klarmann,et al. Discontinuous plus-strand DNA synthesis in human immunodeficiency virus type 1-infected cells and in a partially reconstituted cell-free system , 1997, Journal of virology.
[26] G. Turcatti,et al. Fluorescent labeling of NK2 receptor at specific sites in vivo and fluorescence energy transfer analysis of NK2 ligand-receptor complexes. , 1997, Receptors & channels.
[27] D. Olsen,et al. Expression and purification of retroviral HIV-1 reverse transcriptase. , 1996, Methods in enzymology.
[28] D W Rodgers,et al. The structure of unliganded reverse transcriptase from the human immunodeficiency virus type 1. , 1995, Proceedings of the National Academy of Sciences of the United States of America.
[29] C. Cameron,et al. [13] Purification and characterization of human immunodeficiency virus type 1 reverse transcriptase , 1995 .
[30] P. Schultz,et al. Site-specific incorporation of biophysical probes into proteins. , 1994, Proceedings of the National Academy of Sciences of the United States of America.
[31] W. Beard,et al. Kinetic analysis of template.primer interactions with recombinant forms of HIV-1 reverse transcriptase. , 1993, Biochemistry.
[32] A. D. Clark,et al. Crystal structure of human immunodeficiency virus type 1 reverse transcriptase complexed with double-stranded DNA at 3.0 A resolution shows bent DNA. , 1993, Proceedings of the National Academy of Sciences of the United States of America.
[33] G. Klarmann,et al. Template-directed pausing of DNA synthesis by HIV-1 reverse transcriptase during polymerization of HIV-1 sequences in vitro. , 1993, The Journal of biological chemistry.
[34] P. Boyer,et al. Cassette mutagenesis of the reverse transcriptase of human immunodeficiency virus type 1 , 1992, Journal of virology.
[35] U. RajBhandary,et al. Direct analysis of aminoacylation levels of tRNAs in vivo. Application to studying recognition of Escherichia coli initiator tRNA mutants by glutaminyl-tRNA synthetase. , 1991, The Journal of biological chemistry.
[36] T. Naas,et al. Subunit‐selective mutagenesis indicates minimal polymerase activity in heterodimer‐associated p51 HIV‐1 reverse transcriptase. , 1991, The EMBO journal.
[37] C. J. Noren,et al. In vitro suppression of an amber mutation by a chemically aminoacylated transfer RNA prepared by runoff transcription , 1990, Nucleic Acids Res..
[38] P G Schultz,et al. A general method for site-specific incorporation of unnatural amino acids into proteins. , 1989, Science.
[39] D. Stammers,et al. HIV-1 reverse transcriptase: crystallization and analysis of domain structure by limited proteolysis. , 1988, Biochemistry.
[40] T. Copeland,et al. Characterization of highly immunogenic p66/p51 as the reverse transcriptase of HTLV-III/LAV. , 1986, Science.
[41] S. Hecht,et al. T4 RNA ligase mediated preparation of novel "chemically misacylated" tRNAPheS. , 1984, Biochemistry.
[42] D. Beard,et al. Reverse transcriptase from avian myeloblastosis virus , 1979, Journal of virology.