Site- and subunit-specific incorporation of unnatural amino acids into HIV-1 reverse transcriptase.

[1]  Dominic Esposito,et al.  A novel cell-free protein synthesis system. , 2004, Journal of biotechnology.

[2]  Samuel H. Wilson,et al.  High-level expression and purification of untagged and histidine-tagged HIV-1 reverse transcriptase. , 2004, Protein Expression and Purification.

[3]  P. Schultz,et al.  Expanding the Genetic Code , 2003, Science.

[4]  Peter G Schultz,et al.  An Expanded Eukaryotic Genetic Code , 2003, Science.

[5]  Brian A. Smith,et al.  A new strategy for the site-specific modification of proteins in vivo. , 2003, Biochemistry.

[6]  H. Lester,et al.  Caging proteins through unnatural amino acid mutagenesis. , 2003, Methods in enzymology.

[7]  P. Schultz,et al.  In Vivo Photocrosslinking with Unnatural Amino Acid Mutagenesis , 2002, Chembiochem : a European journal of chemical biology.

[8]  S. Hecht,et al.  Fluorescence resonance energy transfer between unnatural amino acids in a structurally modified dihydrofolate reductase. , 2002, Journal of the American Chemical Society.

[9]  Andrew B. Martin,et al.  Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[10]  P. Schultz,et al.  Adding L-3-(2-Naphthyl)alanine to the genetic code of E. coli. , 2002, Journal of the American Chemical Society.

[11]  K. Terpe Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems , 2002, Applied Microbiology and Biotechnology.

[12]  Liang Xie,et al.  Import of amber and ochre suppressor tRNAs into mammalian cells: A general approach to site-specific insertion of amino acid analogues into proteins , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[13]  P. Schultz,et al.  A general approach for the generation of orthogonal tRNAs. , 2001, Chemistry & biology.

[14]  Takuya Ueda,et al.  Cell-free translation reconstituted with purified components , 2001, Nature Biotechnology.

[15]  P G Schultz,et al.  Expanding the Genetic Code of Escherichia coli , 2001, Science.

[16]  A. D. Clark,et al.  Crystal structure of HIV‐1 reverse transcriptase in complex with a polypurine tract RNA:DNA , 2001, The EMBO journal.

[17]  S. L. Le Grice,et al.  Evaluation of retroviral ribonuclease H activity. , 2001, Methods in molecular biology.

[18]  D. A. Dougherty,et al.  Unnatural amino acids as probes of protein structure and function. , 2000, Current opinion in chemical biology.

[19]  G. F. Short,et al.  Probing the S1/S1' substrate binding pocket geometry of HIV-1 protease with modified aspartic acid analogues. , 2000, Biochemistry.

[20]  T. Schmidt,et al.  Use of the Strep-Tag and streptavidin for detection and purification of recombinant proteins. , 2000, Methods in enzymology.

[21]  G. F. Short,et al.  Effects of release factor 1 on in vitro protein translation and the elaboration of proteins containing unnatural amino acids. , 1999, Biochemistry.

[22]  P. Schultz,et al.  Progress toward the evolution of an organism with an expanded genetic code. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[23]  G L Verdine,et al.  Structure of a covalently trapped catalytic complex of HIV-1 reverse transcriptase: implications for drug resistance. , 1998, Science.

[24]  V. Pandey,et al.  Loss of polymerase activity due to Tyr to Phe substitution in the YMDD motif of human immunodeficiency virus type-1 reverse transcriptase is compensated by Met to Val substitution within the same motif. , 1998, Biochemistry.

[25]  G. Klarmann,et al.  Discontinuous plus-strand DNA synthesis in human immunodeficiency virus type 1-infected cells and in a partially reconstituted cell-free system , 1997, Journal of virology.

[26]  G. Turcatti,et al.  Fluorescent labeling of NK2 receptor at specific sites in vivo and fluorescence energy transfer analysis of NK2 ligand-receptor complexes. , 1997, Receptors & channels.

[27]  D. Olsen,et al.  Expression and purification of retroviral HIV-1 reverse transcriptase. , 1996, Methods in enzymology.

[28]  D W Rodgers,et al.  The structure of unliganded reverse transcriptase from the human immunodeficiency virus type 1. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[29]  C. Cameron,et al.  [13] Purification and characterization of human immunodeficiency virus type 1 reverse transcriptase , 1995 .

[30]  P. Schultz,et al.  Site-specific incorporation of biophysical probes into proteins. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[31]  W. Beard,et al.  Kinetic analysis of template.primer interactions with recombinant forms of HIV-1 reverse transcriptase. , 1993, Biochemistry.

[32]  A. D. Clark,et al.  Crystal structure of human immunodeficiency virus type 1 reverse transcriptase complexed with double-stranded DNA at 3.0 A resolution shows bent DNA. , 1993, Proceedings of the National Academy of Sciences of the United States of America.

[33]  G. Klarmann,et al.  Template-directed pausing of DNA synthesis by HIV-1 reverse transcriptase during polymerization of HIV-1 sequences in vitro. , 1993, The Journal of biological chemistry.

[34]  P. Boyer,et al.  Cassette mutagenesis of the reverse transcriptase of human immunodeficiency virus type 1 , 1992, Journal of virology.

[35]  U. RajBhandary,et al.  Direct analysis of aminoacylation levels of tRNAs in vivo. Application to studying recognition of Escherichia coli initiator tRNA mutants by glutaminyl-tRNA synthetase. , 1991, The Journal of biological chemistry.

[36]  T. Naas,et al.  Subunit‐selective mutagenesis indicates minimal polymerase activity in heterodimer‐associated p51 HIV‐1 reverse transcriptase. , 1991, The EMBO journal.

[37]  C. J. Noren,et al.  In vitro suppression of an amber mutation by a chemically aminoacylated transfer RNA prepared by runoff transcription , 1990, Nucleic Acids Res..

[38]  P G Schultz,et al.  A general method for site-specific incorporation of unnatural amino acids into proteins. , 1989, Science.

[39]  D. Stammers,et al.  HIV-1 reverse transcriptase: crystallization and analysis of domain structure by limited proteolysis. , 1988, Biochemistry.

[40]  T. Copeland,et al.  Characterization of highly immunogenic p66/p51 as the reverse transcriptase of HTLV-III/LAV. , 1986, Science.

[41]  S. Hecht,et al.  T4 RNA ligase mediated preparation of novel "chemically misacylated" tRNAPheS. , 1984, Biochemistry.

[42]  D. Beard,et al.  Reverse transcriptase from avian myeloblastosis virus , 1979, Journal of virology.