A comparative study of two chemical models for creating subsurface caries lesions on aprismatic and prismatic enamel.

PURPOSE To investigate the mineral density and lesion depth of artificial caries lesions on aprismatic enamel and prismatic enamel created by lactic acid and acetic acid buffers. METHODS Forty bovine enamel blocks were allocated to: aprismatic enamel (Group A) and prismatic enamel (Group C) in acetic acid buffer for 192 h and aprismatic enamel (Group B) and prismatic enamel (Group D) in lactic acid buffer for 96 h. The mineral loss and lesion depth were measured using micro-computed tomography. RESULTS A significant difference (P = 0.01) was observed in the mineral loss (%) in the lesions on aprismatic enamel and prismatic enamel treated with lactic acid buffer while no significant difference (P = 0.51) was observed in the mineral loss (%) in the lesions on aprismatic enamel and prismatic enamel treated with acetic acid buffer. No significant difference was noted in the mean lesion depth of lesions on aprismatic enamel and prismatic enamel treated with acetic acid and lactic acid buffers (P > 0.05). CONCLUSION Aprismatic enamel and prismatic enamel have similar mineral loss in acetic acid while prismatic enamel showed more mineral loss compared to aprismatic enamel in lactic acid.

[1]  C. Yiu,et al.  Enamel remineralization potential of arginine-fluoride varnish in a multi-species bacterial pH-cycling model. , 2020, Journal of dentistry.

[2]  E. Lo,et al.  The combined enamel remineralization potential of arginine and fluoride toothpaste. , 2018, Journal of dentistry.

[3]  G. Eckert,et al.  The influence of hardness and chemical composition on enamel demineralization and subsequent remineralization. , 2018, Journal of dentistry.

[4]  M. Mei,et al.  Effects of Fluoride on Two Chemical Models of Enamel Demineralization , 2017, Materials.

[5]  N. King,et al.  Remineralizing potential of a 60‐s in vitro application of Tooth Mousse Plus , 2017, International journal of paediatric dentistry.

[6]  M. Mei,et al.  A Review of the Common Models Used in Mechanistic Studies on Demineralization-Remineralization for Cariology Research , 2017, Dentistry journal.

[7]  T. Lenzi,et al.  Bovine tooth is a substitute for human tooth on bond strength studies: A systematic review and meta-analysis of in vitro studies. , 2016, Dental materials : official publication of the Academy of Dental Materials.

[8]  H. Meyer-Lueckel,et al.  Re- and Demineralization Characteristics of Enamel Depending on Baseline Mineral Loss and Lesion Depth in situ , 2016, Caries Research.

[9]  F. Lippert,et al.  Fluoride dose-response of human and bovine enamel artificial caries lesions under pH-cycling conditions , 2015, Clinical Oral Investigations.

[10]  M. Buzalaf,et al.  Different Protocols to Produce Artificial Dentine Carious Lesions in vitro and in situ: Hardness and Mineral Content Correlation , 2012, Caries Research.

[11]  S. Leal,et al.  Validity of MicroCT for in vitro detection of proximal carious lesions in primary molars. , 2012, Journal of dentistry.

[12]  G. Eckert,et al.  In situ Fluoride Response of Caries Lesions with Different Mineral Distributions at Baseline , 2011, Caries Research.

[13]  D. Purton,et al.  A System of Calibrating Microtomography for Use in Caries Research , 2009, Caries Research.

[14]  T. Attin,et al.  Erosive effects of different acids on bovine enamel: release of calcium and phosphate in vitro. , 2005, Archives of oral biology.

[15]  T. Kodaka.  Scanning electron microscopic observations of surface prismless enamel formed by minute crystals in some human permanent teeth , 2003, Anatomical science international.

[16]  M. Addy,et al.  Erosion of dentine and enamel in vitro by dietary acids: the effect of temperature, acid character, concentration and exposure time. , 2000, Journal of oral rehabilitation.

[17]  B. Amaechi,et al.  Factors influencing the development of dental erosion in vitro: enamel type, temperature and exposure time. , 1999, Journal of oral rehabilitation.

[18]  R. Kent,et al.  Kinetics of Enamel Demineralization in vitro , 1999, Journal of dental research.

[19]  S. Higashi,et al.  Structural and distribution patterns of surface 'prismless' enamel in human permanent teeth. , 1991, Caries research.

[20]  J. Meurman,et al.  Scanning electron microscopic study of the effect of salivary pellicle on enamel erosion. , 1991, Caries research.

[21]  J. Meurman,et al.  Progression and surface ultrastructure of in vitro caused erosive lesions in human and bovine enamel. , 1991, Caries research.

[22]  M. Kuroiwa.  Acid Resistance of Surface ‘Prismless’ Enamel in Human Deciduous and Permanent Teeth , 1990 .

[23]  S. Higashi,et al.  Structure of the so-called 'prismless' enamel in human deciduous teeth. , 1989, Caries research.

[24]  D. K. Whittaker,et al.  Suitability of human, bovine, equine, and ovine tooth enamel for studies of artificial bacterial carious lesions. , 1988, Caries research.

[25]  F. Driessens,et al.  Effect of the pH of buffer solutions on artificial carious lesion formation in human tooth enamel. , 1984, Caries research.

[26]  J M ten Cate,et al.  Comparison of artificial caries-like lesions by quantitative microradiography and microhardness profiles. , 1983, Caries research.

[27]  J. M. ten Cate,et al.  Alternating demineralization and remineralization of artificial enamel lesions. , 1982, Caries research.

[28]  J. Featherstone,et al.  Effect of acetic, lactic and other organic acids on the formation of artificial carious lesions. , 1981, Caries research.

[29]  G. Sperber,et al.  Effect of Different Acids on Character of Demineralization of Enamel Surfaces , 1963, Journal of dental research.

[30]  J. A. Gray.  Kinetics of the Dissolution of Human Dental Enamel in Acid , 1962, Journal of dental research.