In situ reactive extraction of itaconic acid during fermentation of Aspergillus terreus

Abstract As a promising value-added chemical from biomass, itaconic acid has great potential in the replacement of petrochemical-based materials and the production of versatile polymers. To integrate itaconic acid recovery in the fermentation process, the applicability of reactive extraction for in situ product removal was investigated. Initially, the biocompatibility of several solvents was assessed based on their influence on the respiratory activity of A. terreus. As a result, a mixture of the extractant trioctylamine and the diluent isopropyl myristate was chosen and reactive extraction was successfully integrated into the cultivation of A. terreus via solvent addition after 63 h. Thereby, the pH of the culture broth was increased and inhibition by undissociated itaconic acid was reduced. As a consequence, glucose consumption and product formation were considerably improved. In combination with an enhanced amount of glucose, either supplied initially or during the cultivation, this increased the total itaconic acid concentration from 70 to 105 g L-1 referred to the absolute amount of itaconic acid divided by the volume of the aqueous culture broth. Simultaneously, volumetric productivity increased from 0.72 to 0.91 g L-1 h-1. With reactive extraction, therefore, a highly promising method for in situ removal of itaconic acid could be established.

[1]  E. Park,et al.  Biotechnological production of itaconic acid and its biosynthesis in Aspergillus terreus , 2009, Applied Microbiology and Biotechnology.

[2]  Jürgen Hubbuch,et al.  Integrated bioprocesses. , 2005, Current opinion in microbiology.

[3]  H. Heipieper,et al.  Mechanisms of resistance of whole cells to toxic organic solvents , 1994 .

[4]  J. Büchs,et al.  Quantifying the release of polymer additives from single-use materials by respiration activity monitoring , 2013 .

[5]  Z. Su,et al.  Integrated bioprocess for high-efficiency production of succinic acid in an expanded-bed adsorption system , 2011 .

[6]  C. King,et al.  Extraction of carboxylic acids with amine extractants. 2. Chemical interactions and interpretation of data , 1990 .

[7]  A. Bassi,et al.  Investigation of a dual‐particle liquid–solid circulating fluidized bed bioreactor for extractive fermentation of lactic acid , 2008, Biotechnology progress.

[8]  廣野 木下.  麹菌族ノ一新種イタコン酸菌 (Aspergillus itaconicus nov. spec.) ニ就テ (豫報) , 1931 .

[9]  T. Tabuchi,et al.  Accumulation of Itaconic, 2-Hydroxyparaconic, Itatartaric, and Malic Acids by Strains of the Genus Ustilago , 1990 .

[10]  M. Matsumoto,et al.  Extraction equilibria of organic acids with tri-n-octylphosphineoxide , 1990 .

[11]  Anja Kuenz,et al.  Influence of the pH on the itaconic acid production with Aspergillus terreus , 2014, Applied Microbiology and Biotechnology.

[12]  S. Wieczorek,et al.  Continuous production of citric acid with recirculation of the fermentation broth after product recovery , 1997 .

[13]  Anja Kuenz,et al.  Filamentous fungi in microtiter plates—an easy way to optimize itaconic acid production with Aspergillus terreus , 2014, Applied Microbiology and Biotechnology.

[14]  D. I. Wang,et al.  Extractive fermentation for lactic acid production , 1991, Biotechnology and bioengineering.

[15]  A.J.J. Straathof,et al.  The Proportion of Downstream Costs in Fermentative Production Processes , 2011 .

[16]  Jochen Büchs,et al.  Hydromechanical stress in shake flasks: Correlation for the maximum local energy dissipation rate , 2006, Biotechnology and bioengineering.

[17]  D. I. Wang,et al.  Strategies for reducing solvent toxicity in extractive fermentations , 1991, Biotechnology and bioengineering.

[18]  J. Büchs,et al.  Power consumption in shaking flasks on rotary shaking machines: II. Nondimensional description of specific power consumption and flow regimes in unbaffled flasks at elevated liquid viscosity. , 2000, Biotechnology and bioengineering.

[19]  M. Matsumoto,et al.  Synergistic Extraction of Organic Acids with Tri-n-octylamine and Tri-n-butylphosphate , 2001 .

[20]  R. Gill,et al.  Organic acid toxicity, tolerance, and production in Escherichia coli biorefining applications , 2005, Microbial cell factories.

[21]  Shangtian Yang,et al.  Extractive Fermentation for Enhanced Propionic Acid Production from Lactose by Propionibacterium acidipropionici , 1998, Biotechnology progress.

[22]  Guneet Kaur,et al.  Correction: Development of reactive extraction systems for itaconic acid: a step towards in situ product recovery for itaconic acid fermentation , 2014 .

[23]  C. King,et al.  Extraction chemistry of fermentation product carboxylic acids. , 1986, Biotechnology and bioengineering.

[24]  Anja Kuenz,et al.  Process development of itaconic acid production by a natural wild type strain of Aspergillus terreus to reach industrially relevant final titers , 2017, Applied Microbiology and Biotechnology.

[25]  J. H. T. Horst,et al.  Intensified crystallization in complex media: heuristics for crystallization of platform chemicals , 2012 .

[26]  J. Büchs,et al.  Maximum stable drop size measurements indicate turbulence attenuation by aeration in a 3 m3 aerated stirred tank , 2014 .

[27]  Romain Jeantet,et al.  Semicontinuous production of lactic acid in a bioreactor coupled with nanofiltration membranes , 1996 .

[28]  R. Wennersten.  The extraction of citric acid from fermentation broth using a solution of a tertiary amine , 2008 .

[29]  Masayoshi Iwahara,et al.  Novel Method of Lactic Acid Production by Electrodialysis Fermentation , 1986, Applied and environmental microbiology.

[30]  C. Kubicek,et al.  A deficiency of manganese ions in the presence of high sugar concentrations is the critical parameter for achieving high yields of itaconic acid by Aspergillus terreus , 2015, Applied Microbiology and Biotechnology.

[31]  Diwakar Z. Shende,et al.  Reactive Extraction of Itaconic Acid Using Quaternary Amine Aliquat 336 in Ethyl Acetate, Toluene, Hexane, and Kerosene , 2011 .

[32]  Shangtian Yang,et al.  Extractive fermentation for butyric acid production from glucose by Clostridium tyrobutyricum. , 2003, Biotechnology and bioengineering.

[33]  P. Punt,et al.  In-stream itaconic acid recovery from aspergillus terreus fedbatch fermentation , 2013 .

[34]  R. Takors,et al.  Process strategies to enhance pyruvate production with recombinant Escherichia coli: From repetitive fed‐batch to in situ product recovery with fully integrated electrodialysis , 2004, Biotechnology and bioengineering.

[35]  Jochen Büchs,et al.  Itaconic acid--a biotechnological process in change. , 2013, Bioresource technology.

[36]  A. Daugulis,et al.  Solvent Selection Strategies for Extractive Biocatalysis , 1991, Biotechnology progress.

[37]  Carlos Ricardo Soccol,et al.  Downstream process development in biotechnological itaconic acid manufacturing , 2016, Applied Microbiology and Biotechnology.

[38]  J. Büchs,et al.  Characterization of hydromechanical stress in aerated stirred tanks up to 40 m3 scale by measurement of maximum stable drop size , 2014, Journal of biological engineering.

[39]  S. Roth,et al.  Parallel use of shake flask and microtiter plate online measuring devices (RAMOS and BioLector) reduces the number of experiments in laboratory-scale stirred tank bioreactors , 2015, Journal of biological engineering.

[40]  N. Wierckx,et al.  Ustilago maydis produces itaconic acid via the unusual intermediate trans‐aconitate , 2015, Microbial biotechnology.

[41]  R. Lambert,et al.  Weak‐acid preservatives: modelling microbial inhibition and response , 1999, Journal of applied microbiology.

[42]  M. Okabe,et al.  Breeding of Aspergillus terreus mutant TN-484 for itaconic acid production with high yield , 1995 .

[43]  Adrie J J Straathof,et al.  Recovery of carboxylic acids produced by fermentation. , 2014, Biotechnology advances.

[44]  G. T. Tsao,et al.  Simultaneous Production and Recovery of Fumaric Acid from Immobilized Rhizopus oryzae with a Rotary Biofilm Contactor and an Adsorption Column , 1996, Applied and environmental microbiology.

[45]  H. Uslu,et al.  Status of the Reactive Extraction as a Method of Separation , 2015 .

[46]  J. Büchs,et al.  Online respiration activity measurement (OTR, CTR, RQ) in shake flasks , 2004 .

[47]  Diwakar Z. Shende,et al.  Reactive extraction of itaconic acid using tri‐n‐butyl phosphate and aliquat 336 in sunflower oil as a non‐toxic diluent , 2011 .

[48]  Klaus-Dieter Vorlop,et al.  Microbial production of itaconic acid: developing a stable platform for high product concentrations , 2012, Applied Microbiology and Biotechnology.

[49]  H. Larsen,et al.  The mechanism of itaconic acid formation by Aspergillus terreus. 1. The effect of acidity. , 1955, The Biochemical journal.

[50]  W. J. McManamey.  Sauter mean and maximum drop diameters of liquid-liquid dispersions in turbulent agitated vessels at low dispersed phase hold-up , 1979 .

[51]  Shangtian Yang,et al.  A Novel Extractive Fermentation Process for Propionic Acid Production from Whey Lactose , 1992 .

[52]  S. Sandler,et al.  On the thermodynamics of microbial growth processes , 1991, Biotechnology and bioengineering.

[53]  Liming Liu,et al.  Relationship between morphology and itaconic acid production by Aspergillus terreus. , 2014, Journal of microbiology and biotechnology.

[54]  J Büchs,et al.  Power consumption in shaking flasks on rotary shaking machines: I. Power consumption measurement in unbaffled flasks at low liquid viscosity. , 2000, Biotechnology and bioengineering.