Bioink of Multi-Biomaterials with Tyrosine Reinforcement for 3D Bioprinting of Skin Constructs

In this paper, a bioink composed of multiple native materials with tyrosine micro-particle reinforcement for 3D bioprinting of skin scaffolds was developed. The rheological property of the developed bioink was optimized for 3D Bioprinting process. The results showed that Tyrosine micro-particle reinforcement strongly enhanced the mechanical strength. Keratinocyte cell culture showed that the 3D bioprinted skin substitutes made of multi-materials bioink is promising in skin repair.

[1]  T. Mocan,et al.  The role of ultraviolet radiation and tyrosine stimulated melanogenesis in the induction of oxidative stress alterations in fair skin melanocytes. , 2009, Experimental oncology.

[2]  P. Netti,et al.  Endogenous human skin equivalent promotes in vitro morphogenesis of follicle-like structures. , 2016, Biomaterials.

[3]  A. Khademhosseini,et al.  Carbon nanotube reinforced hybrid microgels as scaffold materials for cell encapsulation. , 2012, ACS nano.

[4]  Brian E. Kilfoyle,et al.  Development of paclitaxel-TyroSpheres for topical skin treatment. , 2012, Journal of controlled release : official journal of the Controlled Release Society.

[5]  Jos Malda,et al.  Reinforcement of hydrogels using three-dimensionally printed microfibres , 2015, Nature Communications.

[6]  Changyou Gao,et al.  The roles of knitted mesh-reinforced collagen-chitosan hybrid scaffold in the one-step repair of full-thickness skin defects in rats. , 2013, Acta biomaterialia.

[7]  Paulo Jorge Da Silva bartolo,et al.  3D bioprinting of photocrosslinkable hydrogel constructs , 2015 .

[8]  Hon Fai Chan,et al.  3D Printing of Highly Stretchable and Tough Hydrogels into Complex, Cellularized Structures , 2015, Advanced materials.

[9]  C. Lim,et al.  Tissue scaffolds for skin wound healing and dermal reconstruction. , 2010, Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology.

[10]  Karl R Edminster,et al.  Multi-layered culture of human skin fibroblasts and keratinocytes through three-dimensional freeform fabrication. , 2009, Biomaterials.

[11]  Brian P. Timko,et al.  A two-component pre-seeded dermal-epidermal scaffold. , 2014, Acta biomaterialia.

[12]  Lijie Grace Zhang,et al.  Three-dimensional printing of nanomaterial scaffolds for complex tissue regeneration. , 2015, Tissue engineering. Part B, Reviews.

[13]  B. Yao,et al.  3D bioprinted extracellular matrix mimics facilitate directed differentiation of epithelial progenitors for sweat gland regeneration. , 2016, Acta biomaterialia.

[14]  S. Hsu,et al.  Synthesis of Thermoresponsive Amphiphilic Polyurethane Gel as a New Cell Printing Material near Body Temperature. , 2015, ACS applied materials & interfaces.